Process for preparing heparin sodium through membrane separation

A technology for heparin sodium and membrane separation, applied in the field of preparation of heparin sodium, can solve the problems of large waste of resources, unstable products, environmental pollution, etc., and achieve the effects of reducing processing costs, improving resin utilization, and reducing production costs

Inactive Publication Date: 2014-05-07
HEBEI CHANGSHAN BIOCHEM PHARMA
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  • Abstract
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Problems solved by technology

The traditional process has problems such as low yield, large waste of resources, and unstable products, esp...

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  • Process for preparing heparin sodium through membrane separation

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Embodiment 1

[0025] Example 1: Transfer 835 kg of intestinal mucosa scraped by 760 pig small intestines to a reaction tank, add 5310 kg of tap water, stir evenly, add 185 kg of sodium chloride to the reaction tank, and then use 20% sodium hydroxide aqueous solution After adjusting the pH of the mixed solution to 8.5, add 2190g of 2709 protease, turn on the steam to heat up and time the enzymolysis at 55°C for 5 hours to obtain the enzymolysis solution, then raise the temperature of the enzymolysis solution to 85°C and keep it warm for 17 minutes. After adjusting the pH to 7.0-9.0, cool down to 55°C, then transfer it to the storage tank, turn on the ceramic membrane equipment, adjust the pressure to 0.3MPa, and then filter through a 5nm ceramic membrane for 4 hours to obtain the filtrate. The filtrate is 10kg of protein For feed processing, the filtrate is pumped into an ion exchange column equipped with Rohm and Haas A98 resin at a temperature of 50-55°C for adsorption at a flow rate of 10L...

Embodiment 2

[0026] Embodiment 2: The difference between this embodiment and Embodiment 1 is that 755 pig small intestines are scraped by an intestinal scraper to scrape 810 kg of intestinal mucosa and transferred to the reaction tank, then add 4050 kg of tap water, stir evenly and add 99.5 kg of sodium chloride, Then 20% sodium hydroxide solution to adjust the pH of the mixed solution to 9.0, then add 4320g of 2709 protease, turn on the steam to heat up and time the enzymolysis at 58°C for 3.5 hours to obtain the enzymolysis solution, then heat the enzymolysis solution to 88°C and keep it warm. After 15 minutes, adjust the pH of the enzymolysis solution to 7.0-9.0, then cool down to 57°C, and then transfer the enzymolysis solution to the storage tank. Turn on the ceramic membrane equipment, adjust the pressure to 0.28MPa, and filter through a 5nm ceramic membrane for 6 hours to obtain the filtrate. The filtrate 8.5kg protein is used as feed processing, and the filtrate is pumped into the R...

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Abstract

The invention discloses a process for preparing heparin sodium through membrane separation. The process comprises the steps: intestinal mucosa enzymolysis, ceramic membrane filtration, ion exchange column chromatography, nanofiltraiton membrane concentration, alcohol precipitation and drying, wherein the optimal condition for the intestinal mucosa enzymolysis is formed by the proper proportion of intestinal mucosa to water, enzymolysis temperature and pH value of a system. A protease can take the optimal enzymolysis effect, the structure and activity of heparin are not damaged when the glucosidic bond linked between protein and the heparin is hydrolyzed, meanwhile, fewer low-molecular heparins are generated, the activity of the heparin can be improved by 20%, and the generated low-molecular heparins can be reduced by 10%. Due to the adoption of the ceramic membrane filtration, protein impurities and the heparin in an enzymatic hydrolysate can be effectively separated, so that the heparin loss is greatly reduced and can be reduced by 5-8% as comparison with that of the traditional process, the production cost is reduced, the discharged wastewater can be reduced by 50-60%, the resin utilization ratio can be increased by 40-50%, the consumption of auxiliary materials is low, the quality of the heparin sodium is stable, the process is convenient to operate, and the defects such as high impurity content in the enzymatic hydrolysate, serious waste liquid discharge pollution, high personnel utilization rate and the like are overcome.

Description

technical field [0001] The invention relates to a process for preparing heparin sodium by membrane separation technology. Background technique [0002] Heparin is a kind of glycosaminoglycan widely present in animal organs, small intestinal mucosa, lung and other tissues. Like most mucopolysaccharides, it exists as a protein-bound complex in the body. The anticoagulant activity of this complex varies with With the removal of protein, its activity increases. Heparin sodium has various pharmacological activities such as anticoagulant, anti-inflammatory, anti-allergic, anti-virus, anti-cancer, and regulating blood lipids. [0003] At present, the domestic production of heparin sodium adopts the traditional process, that is, enzymatic hydrolysis of pig small intestine mucosa, dynamic adsorption of resin, dynamic elution, alcohol precipitation, drying and other processes. The traditional process has problems such as low yield, large waste of resources, and unstable products, esp...

Claims

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Application Information

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IPC IPC(8): C08B37/10
Inventor 姬胜利高树华赵利涛王志华
Owner HEBEI CHANGSHAN BIOCHEM PHARMA
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