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Preparation method for novel suckercide

A sprouting inhibitor and a new type of technology are applied in the field of biological new sprouting inhibitor preparation, which can solve the problems of large investment in production equipment, complicated fermentation process, and many waste water and residues, and achieve low production cost, high concentration, and no environmental pollution. Effect

Active Publication Date: 2014-05-14
CHONGQING YINGLONG CHEM PLANT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although its bud-inhibiting effect is high, the culture method of its bacterial strain adopts liquid fermentation, which produces a lot of waste water and waste residue, which is easy to cause environmental pollution, and the concentration of the product is relatively low
In addition, the disclosed invention patent is a liquid fermentation method, the fermentation process is complicated, the investment in production equipment is large, and the threat of bacterial contamination is greater than that of solid-state fermentation. Once contamination occurs, the entire tank is often scrapped, and the operation risk is high.

Method used

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  • Preparation method for novel suckercide

Examples

Experimental program
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Effect test

Embodiment 1

[0029] The invention provides a kind of preparation method of novel sprout inhibitor, comprises the following steps:

[0030] 1) Specimen collection: First collect the diseased specimens of Botrytis Cinerea Persr fungus CGMCC NO0357-1 from the plants. The fungus was preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures in 1998. Fresh specimens with typical symptoms were selected for surface treatment. Disinfection, after rinsing with sterilized water, use tissue separation or direct spore shake-off method to separate and purify single spores;

[0031] 2) Then culture the purified cultured strains in the culture medium. Under the condition of the temperature of the incubator at 24°C, the PDA medium is used for 48 hours. Peptone, its parts by weight are: 2:2:20:1;

[0032] 3) if figure 1 As shown, the following solid fermentation procedure is carried out:

[0033] Primary strain cultivation: Under the condition of aseptic ope...

Embodiment 2

[0043] Except for step 2), the specific preparation steps are the same as in Example 1. The medium used in step 2) is a seed liquid medium, and the medium includes sucrose, peeled potatoes, and peptone, and the weight parts are: 2, 20 ,1.

Embodiment 3

[0045] Except for step 2), the specific preparation steps are the same as in Example 1. The medium used in step 2) is a seed tank medium or a pilot medium, and the medium includes wheat bran, peptone, sucrose, urea, chlorinated Potassium, magnesium sulfate heptahydrate, potassium dihydrogen phosphate, ferrous sulfate heptahydrate, manganese sulfate, sodium nitrate, peanut oil, defoamer, its parts by weight are: 10, 0.05, 1.5, 0.08, 0.0325, 0.0325, 0.0525, 0.00137, 0.0075, 0.0075, 0.2, 0.03, adjust the pH value to about 8.5.

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Abstract

The invention provides a preparation method for a novel suckercide which takes beta-phenethyl alcohol as an active component. The preparation method comprises sample collection, medium culturing and solid fermentation. The solid fermentation comprises first-level strain culture, second-level strain liquid culture, third-level amplification culture, sterilization in a solid fermentation chamber, hyphe solid fermentation, aeration drying, metabolite extraction, crude product obtaining and active component separation. The suckercide prepared by the preparation method has the effective rate up to 92-98%, the whole production process is safe and non-toxic, and by the solid fermentation method, the fermentation process is simple, the production equipment investment is little, the product concentration is high, and risk of contaminating microorganism pollution is small.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a method for preparing a biological novel sprout inhibitor. Background technique [0002] Tobacco leaves are planted in a large area in many provinces of our country, and the economic benefits are obvious. However, capping and pruning in field management every year is very time-consuming and laborious, and new ones will grow again shortly after the pruning buds are removed. In order to increase the output of tobacco leaf products and reduce the labor force, the prices of Flumetralin (suppressing bud sensitivity) and Maleichydrazide (bud enemy) purchased from abroad are relatively high, and they are organic synthetic agents, which have certain residual toxicity to flue-cured tobacco and cause environmental pollution. pollution problem. [0003] According to the search, Chinese invention patent: a biological coating agent and its preparation method and its application in the con...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01P21/00
Inventor 刘令辉
Owner CHONGQING YINGLONG CHEM PLANT
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