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Method for preparing methyl diphenylmethane dicarbamate by microbial fermentation

A technology of methyl dicarbamate and diphenylmethane, applied in the field of microbial fermentation to prepare methyl diphenylmethane dicarbamate, which can solve the problems of severe dependence on petrochemical products, resource constraints, environmental hazards, etc., and achieve extraction The separation and purification process is easy, does not destroy the ecological balance, and has the effect of low risk

Active Publication Date: 2018-04-17
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Plant extraction and separation have the disadvantages of low yield and limited resources; while in the process of chemical synthesis, dangerous chemical reagents such as nitrobenzene and hydrochloric acid are used, which are heavily dependent on petrochemical products and have great harm to the environment (Xie Kewei, Xiang Shuguang, Han Fanghuang. Progress in Synthesis of Methyl Diphenylmethanedicarbamate from Methyl Phenylcarbamate. Chemical Industry and Engineering Technology, 2008, 29(4), 37-42.)

Method used

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  • Method for preparing methyl diphenylmethane dicarbamate by microbial fermentation
  • Method for preparing methyl diphenylmethane dicarbamate by microbial fermentation
  • Method for preparing methyl diphenylmethane dicarbamate by microbial fermentation

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Experimental program
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Embodiment 1

[0020] Embodiment 1: the production technology of the microbial fermentation preparation methyl diphenylmethane dicarbamate of the present invention

[0021] 1. Culture medium:

[0022] 1. Solid medium:

[0023] Yeast extract 4.0g / L, malt extract 10.0g / L, glucose 4.0g / L, agar 20.0g / L, pH natural value

[0024] 2. Shake flask seeds, fermentation medium:

[0025] Soy flour 5g / L, soluble starch 15g / L, peptone 15g / L, glycerin 15g / L, NaCl 5g / L, pH natural value

[0026] 2. Fermentation production

[0027] 1. Plate activation of strains: Open the freeze-dried tube of skim milk for strains, pick a small amount and dilute it in sterile distilled water, then absorb an appropriate amount and spread it evenly on the plate medium, culture at 28°C for 5-7 days, transfer to Store in a refrigerator at 4°C.

[0028] 2. Shake flask seed culture: Dig out the activated plate strains and inoculate into each seed culture bottle, and culture at a constant temperature of 28°C, 210rpm, for 5 day...

Embodiment 2

[0031] Embodiment 2: structural identification of methyl diphenylmethane dicarbamate of microbial origin according to the present invention

[0032] Its structure was determined by mass spectrometry, nuclear magnetic resonance and X-ray single crystal diffraction to be methyl diphenylmethane dicarbamate (4,4'-diphenylmethane-bis(methyl)carbamates, DPMC), molecular formula: C 17 h 18 N 2 o 4 , molecular weight 314, the structural formula is as follows:

[0033]

[0034] Formula I.DMPC chemical structural formula

[0035] Table 1. Compounds of DMPC 1 H(500MHz) and 13 C NMR (100MHz) data (in DMSO-d 6 )

[0036]

[0037]

[0038] Formula II.DMPC crystal chemical structure formula

[0039] Crystal data: C 17 h 18 N 2 o 4 ; r 314.33g mol -1 ;absorption coefficient=0.096mm -1 ;space groupP2(1) / c;triclinic,α=5.1389(4) b=31.402(2) c=9.7516(7) α=90.00°, β=93.9530(10)°, γ=90.00°; V=1569.9(2) Z=4; F(000)=664; λ(MoKα)=0.71073 D. x =1.330g em -3 ,T min ...

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PUM

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Abstract

The invention belongs to the technical field of biological preparations, in particular to a method for preparing methyl diphenylmethane dicarbamate by microbial fermentation. The strain used is Catenulispora acidiphila, deposit number DSM 44928. Its process is as follows: (1) the nutrition in the culture medium adopts carbon source and nitrogen source, and wherein carbon source can be glucose, starch, dextrin, maltose, and nitrogen source can be peptone, peanut powder, soybean powder, beef extract, In addition to fish meal, inorganic salts need to be added; (2) using the production strain, the secondary metabolite - methyl diphenylmethane dicarbamate can be obtained through the method of shake flask secondary fermentation. The specific process includes: the original strain Activation, preparation of shake flask seeds, shake flask fermentation, post-treatment of fermentation broth and mycelium, extraction and purification to prepare methyl diphenylmethane dicarbamate. The present invention uses microbial fermentation to obtain methyl diphenylmethane dicarbamate for the first time, and its structure is proved by nuclear magnetic resonance and X-ray single crystal diffraction, which provides a new method for replacing traditional organic synthesis and plant extraction.

Description

Technical field: [0001] The invention belongs to the technical field of biological preparations, in particular to a method for preparing methyl diphenylmethane dicarbamate by microbial fermentation. Background technique: [0002] Studies have found that methyl diphenylmethane-bis(methyl)carbamates (4,4′-diphenylmethane-bis(methyl)carbamates, DPMC) is a trace component of the traditional Chinese medicine Cortex Mori (Zheng Zhaoguang, Wang Rushang, Tang Dan, et al. Research on the chemical constituents of Morusalba L., Natural Product Research and Development, 2011, 23, 399-400, 403.), Morusalba L. is the dried root bark of the Moraceae plant Morusalba L., sweet, cold, nearly Several years of pharmacological studies have shown that Cortex Morus albicans has the activities of lowering blood sugar and blood pressure, diuresis, antitussive and asthmatic, anti-HIV and anti-tumor (Wu Zhiping, Tan Jianzhong, Gu Zhenlun. Research progress on the chemical constituents and pharmacologi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P13/02C12R1/01
Inventor 刘涛庄以彬周威梁晓梅
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI