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Camellia oleifera cake extract and bacillus thuringiensis compound insecticide and preparation method thereof

A technology of Bacillus thuringiensis and Camellia oleifera cake, applied in botany equipment and methods, insecticides, biocides, etc., can solve the problems of limited osthole sources and compounding restrictions, and achieve the promotion of health and sustainable development, Reduced solvent loss and high purity

Inactive Publication Date: 2015-12-09
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limited source of osthole, the compounding of the two is limited to a certain extent.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 The compound insecticide in this example is prepared from the following raw materials by mass percentage: Camellia oleifera cake extract 60%; Bacillus thuringiensis inoculum 15%; Nonylphenol polyoxyethylene ether 10%; MorwetD-400 10%; Bentonite 5% %.

[0029] The oil-tea camellia cake extract is prepared according to the following method:

[0030] (1) Mixed solvent extraction: bake the camellia oleifera cake at 100°C for 6 hours, pulverize it with a pulverizer, pass through a 200-mesh sieve, and heat the sieved material with a mixture of methanol and acetone (volume ratio: 7:1) Reflux extraction at 75°C for 3 times, each time add methanol and acetone mixed extract volume (ml): sieve mass (g)=7:1, each extraction time is 3 hours, combine each extract Finally, use a rotary evaporator to carry out vacuum distillation to remove methanol and acetone until all the methanol and acetone are volatilized to obtain the extract of methanol and acetone extract;

[0031...

Embodiment 2

[0048] The main difference between this example and Example 1 is that the mass distribution ratio of each raw material is: Camellia oleifera cake extract 50%; Bacillus thuringiensis inoculum 20% (active ingredient content 32000IU / mg; Guangzhou Duoyuduo Biotechnology Co., Ltd.) Calcium dodecylbenzenesulfonate 15%; Hydroxymethylcellulose 5%; Kaolin 10%.

[0049] The insecticidal efficacy of the compound insecticide of this embodiment is shown in Table 4-6.

[0050] (1) Poisonous effect on Spodoptera litura

[0051] Dilute the compound insecticide of Example 2 with water in different times (1000 times, 1500 times, 2000 times), spray it on the surface of cabbage leaves, and then raise live Spodoptera litura on the cabbage leaves for insecticidal efficacy test , and set up 3 groups of control groups in addition: control group 1: do not spray the compound insecticide of embodiment 2; material, calcium dodecylbenzenesulfonate, hydroxymethylcellulose, and kaolin were mixed according...

Embodiment 3

[0063] The difference between this example and Example 1 is that the ratio of the quality components of each raw material is: Camellia oleifera cake extract powder (passed through a 200-mesh sieve) 40%; Bacillus thuringiensis agent (active ingredient content 30000IU / mg) 30%; Sorbitol polyoxyethylene ether 5%; Calcium lignosulfonate 15%; Starch 10%.

[0064] The insecticidal efficacy of the compound insecticide of this embodiment is shown in Table 7-9.

[0065] (1) Poisonous effect on Spodoptera litura

[0066] Dilute the compound insecticide of Example 3 with water in different times (1000 times, 1500 times, 2000 times), spray it on the surface of cabbage leaves, and then raise live Spodoptera litura on cabbage leaves for insecticidal efficacy test , repeated three times, and set up 3 groups of control groups in addition: control group 1: do not spray the compound insecticide of embodiment 3; Extract powder, sorbitol polyoxyethylene ether, calcium lignosulfonate, and starch ...

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PUM

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Abstract

The invention discloses an oil-tea cake extract and bacillus thuringiensis compound insecticide and a preparation method thereof. The compound insecticide is prepared from the following raw materials in percentage by mass: 35-65% of oil-tea cake extract, 10-30% of bacillus thuringiensis fungicide, 4-20% of a surfactant, 4-20% of an assistant and 4-20% of a filler. The invention further discloses a preparation method for the compound insecticide. The compound insecticide is wide in insecticidal spectrum, high in activity, fast and stable in insecticidal efficacy and long in residual life, has good killing and wounding effects on old larvae, and has small influences on the environment. According to the compound insecticide, the additional value of the oil tea can be improved, and the wider application of the bacillus thuringiensis can be promoted.

Description

technical field [0001] The invention relates to a compound insecticide of a botanical insecticide and a microbial insecticide and a preparation method thereof, in particular to a compound insecticide of camellia oleifera cake extract and Bacillus thuringiensis and a preparation method thereof. Background technique [0002] With the development of modern science and technology, chemical pesticides are widely used because of their advantages such as wide adaptability, simple operation, low cost and remarkable effect. However, due to the low insecticidal specificity of chemical pesticides, the residual effect period in the field is long, and at the same time, it is easy to induce pests to develop resistance to them, especially the pollution of agricultural products and the environment caused by residual chemical pesticides, resulting in miscarriage of women, baby deformities and induced human Various cancers and other diseases, so the use of biopesticides to control pests is in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/08A01P7/04A01N63/00
CPCY02A50/30
Inventor 毛绍名章怀云刘高强
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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