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Chitosan-based self-assembled nano micelle solution loaded with beta-carotene and preparation method thereof

A technology of nanomicelle solution and carotene, which is applied in the field of preparation of beta-carotene nanomicelle carrier, can solve the problems of few research reports on ionic liquids, and achieves poor water solubility, chemical stability, and good biodegradability. , the effect of increasing solubility

Active Publication Date: 2014-05-28
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the hydrophobic modification of chitosan is carried out in dilute acidic solution or organic solvent, but there are few research reports on using ionic liquid as solvent.

Method used

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  • Chitosan-based self-assembled nano micelle solution loaded with beta-carotene and preparation method thereof
  • Chitosan-based self-assembled nano micelle solution loaded with beta-carotene and preparation method thereof
  • Chitosan-based self-assembled nano micelle solution loaded with beta-carotene and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Preparation of chitosan-grafted polylactic acid (CS-g-PLA): Weigh 10g of 1-allyl-3-methylimidazole chloride in a 25mL three-neck flask, dissolve in an oil bath at 80°C and add 1g of shell Polysaccharide (CS, molecular weight 50kDa, degree of deacetylation 90%), magnetically stirred for 4h until uniformly dispersed; then slowly added 1.8g lactide and 100μL stannous octoate, heated to 130°C for 24h under nitrogen protection; after the reaction, Precipitate with absolute ethanol, discard the supernatant after centrifugation, and wash the collected precipitate with ethanol for 4 times; then extract with acetone for 24 hours in a Soxhlet extractor; finally vacuum the extracted product at 45°C Dry for 24 hours to obtain CS-g-PLA, whose reaction equation is:

[0034]

[0035] Identification of CS-g-PLA: In the infrared spectrum, at 1743cm -1 A new absorption peak appeared at 2930cm -1 and 2880cm -1 The C-H stretching vibration absorption peaks of the methyl group and...

Embodiment 2

[0041] (1) Preparation of CS-g-PLA: Weigh 10g of 1-allyl-3-methylimidazole chloride into a 25mL three-neck flask, dissolve in an oil bath at 80°C, and add 1g of CS (molecular weight: 50kDa, degree of deacetylation: 90% ), magnetically stirred for 4 hours until evenly dispersed; then slowly added 1.8g of lactide and 100μL of stannous octoate, and heated to 130°C under nitrogen protection for 24 hours of reaction; after the reaction, precipitated with absolute ethanol and washed repeatedly; Extracted for 24 hours; finally, the extracted product was vacuum-dried at 45°C for 24 hours.

[0042] (2) Preparation of drug-loaded nanomicelle solution: Dissolve the chitosan-grafted polylactic acid prepared in step (1) in ultrapure water; weigh 10 mg of β-carotene and dissolve it in 2 mL of tetrahydrofuran, and slowly add it dropwise under magnetic stirring into 10mL of 1mg / mL CS-g-PLA aqueous solution; after ultrasonication for 2h (output power 75W, pulse width 2.0s, intermittent time 2....

Embodiment 3

[0047] (1) Preparation of CS-g-PLA: Weigh 10g of 1-butyl-3-methylimidazole chloride into a 25mL three-neck flask, dissolve in an oil bath at 80°C and add 1g of CS (molecular weight: 100KDa, degree of deacetylation: 85%) , magnetically stirred for 4 hours until uniformly dispersed; then slowly added 1.8g of lactide and 0.758g of 4-dimethylaminopyridine, and heated to 130°C for 24 hours under nitrogen protection; after the reaction, precipitated with absolute ethanol, centrifuged and discarded The supernatant and the collected precipitate were washed with ethanol four times; then extracted with acetone for 24 hours; finally, the extracted product was vacuum-dried at 45° C. for 24 hours.

[0048] (2) Preparation of drug-loaded nanomicelle solution: Dissolve the chitosan-grafted polylactic acid prepared in step (1) in ultrapure water; weigh 0.6 mg of β-carotene and dissolve it in 2 mL of tetrahydrofuran, and slowly drop it under magnetic stirring Add to 10mL of 1mg / mL CS-g-PLA aqu...

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Abstract

The invention discloses a chitosan-based self-assembled nano micelle solution loaded with beta-carotene and a preparation method thereof. An imidazoles ionic liquid is used as an effective solvent of chitosan, and under the action of a catalyst, lactide is subjected to ring opening polymerization and is grafted to a chitosan macromolecular chain to obtain a chitosan-grafted polylactic acid; then a self assembly action of the chitosan-grafted polylactic acid in an aqueous solution is utilized to achieve effective embedding of hydrophobic beta-carotene. The water solubility and the oxidation resistance of beta-carotene can be effectively improved, moreover, the preparation process is simple, and conditions are mild; and the prepared nano micelle solution has good stability, and is expected to provide a natural and effective stable carrier and a water-phase dispersant for the cosmetic field or the functional food field.

Description

technical field [0001] The invention belongs to the technical field of nanocarriers, and relates to a method for hydrophobizing modification using natural and biodegradable chitosan as a raw material, in particular to a preparation method and application of a β-carotene nanomicelle carrier, which is used for cosmetics Industry and functional food industry provide a natural and effective stable carrier and aqueous dispersant. Background technique [0002] β-carotene is the most ubiquitous natural pigment in nature and an indispensable nutrient for maintaining human health. At the same time, as an effective antioxidant, it can effectively scavenge free radicals in the human body, prevent skin aging and tissue damage, so it has increasingly received widespread attention in the fields of cosmetics and food. However, β-carotene is insoluble in water and has low bioavailability. Its rich conjugated double bond structure makes it more sensitive to external factors such as light an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/06A61K8/31A61K8/73A61Q19/08A23L1/29A23P1/04A23L33/00
Inventor 王小慧葛文娇孙润仓
Owner SOUTH CHINA UNIV OF TECH
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