Preparation method of ionic-bond cross-linked polymer vesicles

A technology for cross-linking polymers and ionic bonds

Inactive Publication Date: 2014-05-28
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation of vesicles by the traditional polymer self-assembly method often requires the use of organic solvents, which limits its application in the biomedical field

Method used

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  • Preparation method of ionic-bond cross-linked polymer vesicles

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Dissolve 1.0 g of sodium alginate and 2.0 g of N-isopropylacrylamide in 30 mL of distilled water, stir well and mix evenly, vacuumize, blow nitrogen, and add potassium persulfate with a mass concentration of 3% and Each 10 mL of sodium sulfite solution was reacted for 24 hours in a water bath at 40° C. under magnetic stirring. The reaction solution was poured into 100 mL of acetone for precipitation, and suction filtered to obtain a crude product. The crude product was extracted in a Soxhlet extractor with 80 mL of methanol for 24 hours, and dried in a vacuum oven for 24 hours to obtain sodium alginate grafted poly-N-isopropylacrylamide.

[0018] Take 0.1 g of sodium alginate grafted poly-N-isopropylacrylamide prepared above and dissolve it in distilled water to make a solution with a concentration of 0.5 mg / mL. Calcium chloride solution per mL was slowly dropped into the above solution, and continued to stir for 24 hours to obtain Ca 2+ Cross-linked polymersomes.

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Embodiment 2

[0021] Dissolve 1.0 g of sodium alginate and 3.0 g of N-isopropylacrylamide in 40 mL of distilled water, stir and mix well, vacuumize, blow nitrogen, and slowly add potassium persulfate with a mass concentration of 3% under nitrogen protection and sodium sulfite solution (12 mL each), placed in a water bath at 40° C., and reacted for 24 hours under magnetic stirring. The reaction solution was poured into 100 mL of acetone for precipitation, and suction filtered to obtain a crude product. The crude product was placed in a Soxhlet extractor, extracted with 100 mL of methanol for 24 hours, and dried in a vacuum oven for 24 hours to obtain poly-N-isopropylacrylamide grafted with sodium alginate.

[0022] Take 0.1 g of the sodium alginate grafted poly-N-isopropylacrylamide prepared above and dissolve it in distilled water to form a solution with a concentration of 1.0 mg / mL. Dissolve 0.02 g of 5-fluorouracil in the above solution. Slowly drop 1.0 mg / mL calcium chloride solution in...

Embodiment 3

[0024] Dissolve 2.0g of sodium alginate and 4.0g of N-isopropylacrylamide in 50ml of distilled water, stir and mix well, vacuumize, blow nitrogen, and slowly add potassium persulfate with a mass concentration of 3% and Each 12 mL of sodium sulfite solution was placed in a water bath at 40° C. and reacted for 24 hours under magnetic stirring. The reaction solution was poured into 150 mL of acetone for precipitation, and suction filtered to obtain a crude product. The crude product was placed in a Soxhlet extractor, extracted with 100 mL of methanol for 24 hours, and dried in a vacuum oven for 24 hours to obtain a sodium alginate grafted poly-N-isopropylacrylamide copolymer.

[0025] Sodium alginate grafted poly-N-isopropylacrylamide prepared above was dissolved in distilled water to prepare a solution with a mass concentration of 0.5 mg / mL. Under stirring conditions, 0.02 The aluminum chloride solution of g doxorubicin was slowly dropped into the above solution, and continued ...

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Abstract

The invention discloses a preparation method of ionic-bond cross-linked polymer vesicles. The preparation method comprises the specific steps of firstly, by taking sodium alginate and N-isopropylacrylamide as the major raw materials, and then preparing a temperature and pH value sensitive graft copolymer, namely, sodium alginate grafted poly-N-isopropylacrylamide through free radical polymerization. The graft copolymer is prepared into a solution with a certain concentration, and then the solution is mixed with a metal ion solution (CaCl2, AlCl3 or SnCl4) different in concentration with the solution under a stirring condition, and the ionic-bond cross-linked polymer vesicles are obtained through self-assembly under the electrostatic interaction between negative and positive ions. The preparation method of the polymer vesicles is characterized in that the metal ions are taken as the cross-linking agent to avoid toxic and side effects caused by chemical cross-linking; the preparation method is mild in preparation conditions, simple and easy to implement; the raw materials are rich in source and non-toxic, and has excellent biocompatibility and degradability and further can be taken as a drug carrier for therapeutic agents to support drugs different in hydrophily and hydrophobicity. The ionic-bond cross-linked polymer vesicles prepared by the preparation method have the particle size in the range from 100 to 350nm and the minimum critical phase transformation temperature ranging from 34.5 to 38.2 DEG C.

Description

technical field [0001] The invention relates to a preparation method of ionomer cross-linked polymer vesicles. The preparation method of this vesicle and the ionomer cross-linked polymer vesicles can be used as a pharmaceutical carrier for a therapeutic agent, and belong to drugs in biomedicine carrier field. Background technique [0002] Polymer vesicles are a class of polymer aggregates formed by the self-assembly of block copolymers, graft copolymers or dendrimers with amphiphilic structures. The double-membrane structure of polymersomes has good compatibility with the cell membrane. As a drug carrier, it can not only reduce the side effects and irritation of the drug, but also maintain the physiological activity of the drug, achieving the purpose of low toxicity, high efficiency and controlled release. , is an ideal drug delivery vehicle in vivo. Compared with small molecule liposomes, polymersomes have the advantages of higher structural stability, designable permeabi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08L51/02C08F251/00C08F220/54A61K9/51A61K47/36
Inventor 李桂英戚旻熠于娜娜
Owner LUDONG UNIVERSITY
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