Microbial starter for treating municipal sludge and preparation method thereof

A technology of microbial starter and urban sludge is applied in the field of fermented organisms to achieve the effects of being beneficial to urban beautification, enriching nutrients and improving absorption and utilization rate.

Inactive Publication Date: 2014-06-04
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using biological fermentation to solve urban sludge is a feasible and non-polluting method, but there is no fermentation agent specifically for urban sludge

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0020] A preparation method of a microbial starter for treating urban sludge, including the following steps:

[0021] Step 1. Preparation of soybean extract

[0022] (1) Wash the soybeans, dry them, smash them, and filter them with a screen with a diameter of 1 mm to obtain soybean powder for use;

[0023] (2) Add soybean powder to a phosphate buffer with a pH of 5.2 and a molar concentration of 0.05 mol / L, and stir well to prepare a mixed solution I. The volume of phosphate buffer corresponding to each gram of soybean powder is 10 mL;

[0024] (3) Put the mixture I prepared in step (2) into a centrifuge with a rotation speed of 6000r / min and centrifuge for 30 minutes, and then dissolve the precipitate in a phosphate buffer with a pH of 6.5 and a molar concentration of 0.05mol / L Then transfer to a centrifuge with a rotation speed of 4000r / min and centrifuge for 15 minutes, and collect the supernatant of the two centrifugation to obtain soybean extract for use;

[0025] Step 2: Weigh ea...

Embodiment 1

[0029] A microbial starter for treating urban sludge, the microbial starter is composed of soybean extract, Bacillus subtilis, silicate bacteria, Trichoderma viride, Lactococcus lactis, Actinomycetes, Bacillus glaci, Flavus oryzae , Yeast, Azotobacter chroococcus, cellulase, protease and phosphorylase, and the weight of each raw material required to prepare the starter is 20 parts of soybean extract, 1 part of Bacillus subtilis, silicate 3 parts of bacteria, 2 parts of Trichoderma viride, 8 parts of Lactococcus lactis, 2 parts of Actinomycetes, 1 part of Bacillus mucilaginous, 2 parts of Flavobacterium oryzae, 3 parts of yeasts, 10 parts of Azotoxin-fixing bacteria, cellulase 8 parts, 8 parts protease and 5 parts phosphorylase.

[0030] A preparation method of a microbial starter for treating urban sludge, including the following steps:

[0031] Step 1. Preparation of soybean extract

[0032] (1) Wash the soybeans, dry them, smash them, and filter them with a screen with a diameter...

Embodiment 2

[0039] A microbial starter for treating urban sludge, the microbial starter is composed of soybean extract, Bacillus subtilis, silicate bacteria, Trichoderma viride, Lactococcus lactis, Actinomycetes, Bacillus glaci, Flavus oryzae , Yeast, Azotobacter chroococcus, cellulase, protease and phosphorylase, and the weight of each raw material required to prepare the starter is 25 parts of soybean extract, 2 parts of Bacillus subtilis, silicate 4 parts of bacteria, 4 parts of Trichoderma viride, 12 parts of Lactococcus lactis, 4 parts of actinomycetes, 2 parts of Bacillus gliadin, 2 parts of Flavobacterium oryzae, 6 parts of yeast, 13 parts of Azotobacter chroococcus, cellulase 12 parts, 12 parts protease and 6 parts phosphorylase.

[0040] A preparation method of a microbial starter for treating urban sludge, including the following steps:

[0041] Step 1. Preparation of soybean extract

[0042] (1) Wash the soybeans, dry them, smash them, and filter them with a screen with a diameter o...

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PUM

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Abstract

The invention belongs to the technical field of fermentative organisms, and particularly relates to a microbial starter for treating municipal sludge and a preparation method thereof. By adding a soybean extract into the starter, the activity of microorganisms and enzymes in the starter is increased greatly, the temperature rising and fermentation time in a substrate fermentation process is shortened, the nutrients after fermentation are enriched, and the working efficiency is increased. The soybean extract is mixed together with complex liquid after ultrasonic treatment, so that components in the complex liquid and the soybean extract are mixed uniformly, the absorption and utilization ratios of the starter by the substrate are increased, and substrate fermentation is more complete. Moreover, ultrasonic treatment is performed intermittently, so that damages of ultrasonic waves to components in the soybean extract are prevented. A preparation process is simple, multiple nutrient substances in the municipal sludge can be decomposed in combination with multiple microorganisms and enzymes, the effect of changing waste into valuable is achieved, urban beautification is promoted, and a nutritional substrate is provided for the growth of plants.

Description

Technical field [0001] The invention belongs to the technical field of fermentation biology, and specifically relates to a microbial starter for treating urban sludge and a preparation method thereof. Background technique [0002] The dewatered sludge of a municipal sewage plant is a solid waste produced by the treatment of wastewater in a municipal sewage plant. With the increase in the number of sewage plants under construction in my country, the sludge output will still increase. Municipal sludge contains a large amount of organic matter and nutrients required by plants such as N, P, and K, which can usually be achieved by forced ventilation and aerobic static composting. However, one fermentation basically takes 30 days. For such large amounts of waste There is a shortcoming of long fermentation time, so it is necessary to explore an economical and effective rapid decomposing method to solve the problem of urban sludge. [0003] Biotechnology is an emerging discipline develop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C12N9/42C12N9/50C12N9/10C02F11/02C12R1/125C12R1/01C12R1/885
Inventor 常会庆徐艳珍王浩王世华
Owner HENAN UNIV OF SCI & TECH
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