Feed additive, preparation method and application of feed additive

A feed additive and material-water ratio technology, applied in biochemical equipment and methods, application, animal feed, etc., can solve the adverse effects on animal health and production performance, hinder the digestion, absorption and metabolism of soybean meal feed nutrients, limit the application ratio, etc. To improve the utilization rate and nutritional value, solve the shortage of raw materials, and promote the growth of animals

Inactive Publication Date: 2014-06-11
GUANGZHOU YOURUI BIOSCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existence of these anti-nutritional factors hinders the digestion, absorption and metabolism of soybean meal feed nutrients by livestock and poultry, and has adverse eff

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0030] The preparation method of the feed additive of one embodiment, comprises the following steps:

[0031] In step 1, the activated Lactobacillus strain is transferred from glycerol to sterilized MRS medium, and cultured at 30°C-37°C for 16-28 hours to obtain seed liquid.

[0032] The Lactobacillus used in this embodiment was purchased from the China General Microorganism Culture Collection and Management Center. The Lactobacillus is selected from Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus casei, Lactobacillus cellobiosus, Lactobacillus fermentum, Lactobacillus delbrueckii subspecies lactic acid, Lactobacillus delbrueckii subsp. One or more of Lactobacillus paracasei and Lactobacillus paracasei. Preferably, the lactobacillus is Lactobacillus fermentum, Lactobacillus plantarum or Lactobacillus acidophilus. When adopting above-mentioned three kinds of lactobacilli to prepare feed additives respectively, the total number of eff...

Embodiment 1

[0046] A preparation method of a feed additive, comprising the following steps:

[0047] Step 1, activate the Lactobacillus fermentum bacterial classification, obtain the activated Lactobacillus fermentum bacterial classification, then transfer the activated Lactobacillus fermentum bacterial classification from glycerol to the test tube of the sterilized MRS medium for cultivation, in 30 Cultivate at ℃ for 28 hours to obtain seed solution. The inoculum volume is 5% (V / V).

[0048] Step 2: Carry out primary expansion culture of the seed solution obtained in step 1 in the MRS medium, and cultivate at 30° C. for 24 hours to obtain the primary seed solution.

[0049] Step 3: The primary seed solution in step 2 is subjected to secondary expansion culture in MRS medium, and cultured at 30° C. for 24 hours to obtain the secondary seed solution.

[0050] The components of the MRS medium used in steps 1, 2, and 3 are:

[0051] Peptone 10g, beef extract 10g, yeast extract 5g, glucose...

Embodiment 2

[0058] A preparation method of a feed additive, comprising the following steps:

[0059] Step 1, activate the Lactobacillus plantarum bacterial classification to obtain the activated Lactobacillus plantarum bacterial classification, then transfer the activated Lactobacillus plantarum bacterial classification to the test tube of the sterilized MRS medium from glycerol and cultivate it at 37 Cultivate at ℃ for 16 hours to obtain seed solution. The inoculum volume is 3% (V / V)

[0060] Step 2. The seed liquid obtained in step 1 is subjected to primary expansion culture in MRS medium, and cultured at 37° C. for 16 hours to obtain the primary seed liquid.

[0061] Step 3: The primary seed solution in step 2 is subjected to secondary expansion culture in MRS medium, and cultured at 37° C. for 24 hours to obtain the secondary seed solution. The components of the MRS medium used in steps 1, 2, and 3 are:

[0062] Peptone 10g, beef extract 10g, yeast extract 5g, glucose 20g, K 2 HPO...

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Abstract

The invention discloses a feed additive, a preparation method and application of the feed additive. The preparation method of the feed additive comprises the steps of transferring activated lactobacillus strains to a sterilized MRS culture medium from glycerinum for culture to obtain a seed solution; carrying out primary and secondary enlarged cultivation on the seed solution in the MRS culture medium, inoculating into the sterilized solid culture medium for solid feed fermentation through an intermittent oxygen-introduction facultative anaerobic fermentation mode, and drying to obtain the feed additive. According to the preparation method of the feed additive, lactobacillus fermented soybean meal, bran, rice bran, straw, cottonseed cake, corncob powder, bagasse and other agricultural wastes are utilized for effectively reducing the antinutritional factor level in the raw materials, and the nutrition utilization rate is increased; the prepared feed additive is rich in antimicrobial peptide and other prebiotic substances secreted by lactobacillus through fermentation and metabolic processes, thereby having a prebiotic function and being healthy. The feed additive can be applied to an animal feed.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a feed additive and its preparation method and application. Background technique [0002] The production and application of microbial fermented feed has a long history in various forms. As early as the early 1960s, foreign countries have begun to develop microbial feed. [0003] Compared with traditional feed, fermented feed is generally reprocessed by using agricultural waste such as soybean meal, bran, rice bran, crop straw, and cottonseed cake. Among them, soybean meal has the characteristics of good palatability, high protein content of more than 43%, reasonable amino acid composition, high digestion and utilization rate, low price compared with animal protein sources such as fish meal and meat and bone meal, and is not easy to be oxidized and corrupted. An important protein feed resource, widely used in compound feed for livestock, poultry and aquatic animals. [000...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/16A23K1/14C12N1/20C12R1/25C12R1/23C12R1/245C12R1/225
CPCY02P60/87
Inventor 刘立国刘小阳陈博
Owner GUANGZHOU YOURUI BIOSCI
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