A biocompatibility-enhanced artificial bone scaffold material and its preparation method

A technology of biocompatibility and scaffold materials, which is applied in the field of biocompatibility-enhanced artificial bone scaffold materials and its preparation, can solve the problems of inconsistent micropore diameter, small pore diameter, growth and vascular tissue ingrowth, etc., to achieve The effect of reducing safety hazards, reducing adhesion, and improving biocompatibility

Active Publication Date: 2016-05-25
SHAANXI GIANT BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There have been related studies on the construction of collagen-hydroxyapatite scaffold materials, but the preparation process is cumbersome and complicated, which is not conducive to scale-up production, or the micropore diameter does not meet the corresponding technical requirements (50-150??m), and the pore diameter Relatively small, not conducive to inward climbing, growth of cells and ingrowth of vascular tissue

Method used

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  • A biocompatibility-enhanced artificial bone scaffold material and its preparation method
  • A biocompatibility-enhanced artificial bone scaffold material and its preparation method
  • A biocompatibility-enhanced artificial bone scaffold material and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Step 1: dissolving collagen in deionized water to prepare a collagen solution with a mass fraction of 20%.

[0038] Collagen is full-length collagen, collagen polypeptide, and gelatin extracted from animal tissues.

[0039] Step 2: Adjust the pH value of the collagen solution to 5.

[0040] The pH adjusting agent is selected from sodium hydroxide solution and hydrochloric acid solution, and the molar volume concentration is 0.01-1mol / L.

[0041] Step 3: Add transglutaminase at a ratio of 2 U / g to collagen.

[0042] Step 4: adding nano-hydroxyapatite according to the mass ratio of collagen to nano-hydroxyapatite at a ratio of 1:2 to obtain a viscous emulsion.

[0043] Step 5: Transfer the viscous emulsion in Step 4 to a mold, react at 30° C. for 24 hours, and let it cool down.

[0044] Step 6: Carry out enzyme inactivation at 90°C for 5 minutes, and place to cool.

[0045] Step 7: Pre-freeze in an ultra-low temperature refrigerator at -80°C for 3 hours, and then vacu...

Embodiment 2

[0047] Step 1: dissolving collagen in deionized water to prepare a collagen solution with a mass fraction of 30%.

[0048] Collagen is recombinant collagen and human-like collagen produced by genetic engineering.

[0049] Step 2: Adjust the pH value of the collagen solution to 6.

[0050] The pH regulator is selected from potassium hydroxide solution and sulfuric acid solution, and the molar volume concentration is 0.01-1mol / L.

[0051] Step 3: Add transglutaminase according to the ratio of transglutaminase to collagen of 8 U / g.

[0052] Step 4: adding nano-hydroxyapatite according to the mass ratio of collagen to nano-hydroxyapatite at a ratio of 1:3 to obtain a viscous emulsion.

[0053] Step 5: Transfer the viscous emulsion in Step 4 to a mold, react at 40°C for 30 hours, and let it cool.

[0054] Step 6: Carry out enzyme inactivation at 90°C for 5 minutes, and place to cool.

[0055] Step 7: Pre-freeze in an ultra-low temperature refrigerator at -80°C for 3 hours, and th...

Embodiment 3

[0057] Step 1: dissolving collagen in deionized water to prepare a collagen solution with a mass fraction of 40%.

[0058] Collagen is recombinant collagen and human-like collagen produced by genetic engineering.

[0059] Step 2: Adjust the pH value of the collagen solution to 7.

[0060] The pH adjusting agent is selected from sodium carbonate solution and phosphoric acid solution, and the molar volume concentration is 0.01-1mol / L.

[0061] Step 3: Add transglutaminase according to the ratio of transglutaminase to collagen of 15 U / g.

[0062] Step 4: adding nano-hydroxyapatite according to the mass ratio of collagen to nano-hydroxyapatite as 1:4 to obtain a viscous emulsion.

[0063] Step 5: Transfer the viscous emulsion in Step 4 to a mold, react at 55° C. for 36 hours, and let it cool down.

[0064] Step 6: Carry out enzyme inactivation at 90°C for 5 minutes, and place to cool.

[0065] Step 7: Pre-freeze in an ultra-low temperature refrigerator at -80°C for 3 hours, an...

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Abstract

The invention relates to a preparation method of an artificial bone scaffold material. In application of bone tissue engineering, a cross-linking agent is usually used to improve anti-pressure ability, degradation property and the like, of an artificial bone, but chemical cross-linking agents such as glutaraldehyde and carbodiimide are usually used at present, the chemical cross-linking agents usually have a toxicity problem and have potential safety hazard for hosts. According to the preparation method, enzyme- transglutaminase is used as a cross-linking agent to improve water solubility and mechanical strength of the prepared collagen / nano hydroxyapatite artificial bone, and inactivation treatment is carried out onto the enzyme after the reaction. In the whole preparation process, no substances or reagents which are toxic and harmful or have potential hazard are introduced, so that biocompatibility of the artificial bone material is greatly improved, the related reactants are free of biological toxicity, and possible potential safety hazards of products on body are reduced; meanwhile, osteoconduction of the hydroxyapatite is also kept, and cell production promotion performance of the collagen is not affected.

Description

technical field [0001] The invention relates to a biomedical material, in particular to an artificial bone scaffold material with enhanced biocompatibility and a preparation method thereof. Background technique [0002] In orthopedic surgery and biomedical engineering, the preparation of bone scaffold materials with excellent performance is still one of the key issues. As a human bone implant material, the scaffold needs to provide a microenvironment similar to that of natural bone, which is conducive to cell adhesion, proliferation and differentiation, and can guide the growth of blood vessels. It has high biological activity and cannot cause immune rejection and inflammation of the body. reaction. [0003] Biocompatibility is the core factor to be considered in the preparation of scaffold materials. The factors affecting the biocompatibility of scaffold materials include not only the structure of the scaffold material itself, but also the physical and chemical properties...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/45A61L27/42A61L27/56A61L27/58
Inventor 范代娣马晓轩惠俊峰范慧米钰
Owner SHAANXI GIANT BIOTECHNOLOGY CO LTD
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