Application of lactobacillus rhamnosus derived from longevity village in Bama of Guangxi in assisting in reducing blood fat
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A Lactobacillus rhamnosus, a technology for assisting blood lipid lowering, applied in the biological field, can solve problems such as major side effects
Active Publication Date: 2014-07-02
ROYAL GRP CO LTD +1
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[0002] With the improvement of people's living standards, the incidence of hyperlipidemia and the cardiovascular diseases cau
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Embodiment 1
[0009] Example 1: strain screening and identification
[0010] Relevant media and reagent formulations
[0011] (1) MRS-THIO medium: peptone 10.0g, sodium acetate trihydrate 3.0g, beef extract 8.0g, triammonium citrate 2.0g, yeast extract 4.0g, magnesium sulfate heptahydrate 0.2g, glucose 10.0g, Manganese sulfate water 0.04g, Tween-80 1.0mL, dipotassium hydrogen phosphate 2.0g, sodium thioglycolate 2.0g, adjust pH to 6.4, sterilize at 121°C for 15min, cool for later use.
[0012] (3) Cholesterol medium: cholesterol 0.10g, Tween-80 1.0mL, sucrose ester 0.10g, glacial acetic acid 5.0mL, ultrasonically crushed in an ice bath for later use. The cholesterol micelle solution prepared above was added into the MRS liquid medium, so that the final concentration of cholesterol in the medium was 0.10 mg / mL. Adjust the pH to 6.50±0.20, sterilize at 121°C for 15 minutes, and cool down for later use.
[0077] Example 2: In vivo auxiliary blood lipid-lowering efficacy experiment
[0078] Using animal experiments to study hsryfm1301 and its fermented milk's auxiliary blood lipid lowering effect in vivo. The high-fat rat model was established by feeding high-fat diet, and the total cholesterol (TC), triglyceride (TG) and high-density lipoprotein cholesterol (HDL-C) in rat serum were determined after gavage of hsryfm1301 and its fermented milk And low-density lipoprotein cholesterol (LDL-C) content, cholesterol, triglyceride content in liver and cholesterol, triglyceride, bile acid content in feces, so as to detect the in vivo auxiliary blood lipid lowering effect of hsryfm1301.
[0079] 1 Experimental animals and groups
[0080] 52 SPF grade male Wistar rats with a body weight of about 120-140g were fed under 23±1°C, dry, clean and well-ventilated indoor conditions, with basic feed (20% flour, 10% rice flour, 20% corn, drumsticks, etc.) Skin 26%, soybean meal 20%, fish meal 2...
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Abstract
The invention relates to the field of biotechnology, and particularly relates to an application of lactobacillus rhamnosus derived from longevity village in Bama of Guangxi in assisting in reducing blood fat. The animal experiment is carried out on the lactobacillus rhamnosus hsryfm1301 with the CGMCC No.8545, which is derived from the longevity village in Bama of Guangxi and has the capacity of externally reducing cholesterol and decomposing triglyceride; the function of assisting in reducing blood fat and the safety of the lactobacillus rhamnosus hsryfm1301 are evaluated. The result shows that afte hsryfm1301 is continuously fed to a rat for 7 days by gavage, the growing state of the rat is normal, the rat has smooth hair and active spirit, and the bacterial strain does not cause translocation and the like of liver sausages. The research on the functional characteristic of assisting in reducing blood fat in the experimental rat shows that the lactobacillus rhamnosus hsryfm1301 and acidified milk thereof are capable of reducing accumulation of fat in the liver of the rat and low density lipoprotein of serum, and the lactobacillus rhamnosus hsryfm1301 has the function of assisting in reducing blood fat.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to the probiotic Lactobacillus rhamnosus hsryfm1301 derived from Changshou Village, Bama, Guangxi, and its application in assisting the blood lipid-lowering function. technical background [0002] With the improvement of people's living standards, the incidence of hyperlipidemia and the cardiovascular diseases caused by it is also increasing year by year. Drugs, as the main way to treat these diseases, have relatively large side effects. Lactic acid bacteria are important probiotics in the human intestinal tract, which play a vital role in maintaining the micro-ecological balance of the host intestinal tract and improving the function of the immune system. In some longevity towns such as the Caucasus mountains and the Mediterranean coast, people often drink homemade Yogurt rarely suffers from cardiovascular and obesity diseases caused by hyperlipidemia; in recent years, a large number o...
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