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Biosensor and manufacturing method for same

A technology of a biosensor and a production method, applied in the field of biosensors and its production, capable of solving the problems of operator safety threats, prone to accidents, high risk, etc., achieving short preparation time, improved electron transfer efficiency, and good uniformity Effect

Inactive Publication Date: 2014-07-16
GUANGDONG TESTING INST OF PROD QUALITY SUPERVISION +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the biggest disadvantage of this method is that it uses explosive and flammable raw materials such as ammonium nitrate and nitroglycerin. During the operation, especially when burning, if the experimental conditions are not well controlled, accidents are prone to occur, which poses a safety threat to operators. higher sex

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for making a biosensor, comprising the steps of:

[0033] 1) Preparation of conductive metal-doped metal oxide nanoparticles.

[0034] First, weigh 6.0 g (0.0286 mol) of citric acid monohydrate, add it into a beaker containing 200 ml of deionized water, and heat and stir at 80° C. until the citric acid is fully dissolved. Then add 5ml of concentrated nitric acid (mass fraction is about 65%, density is about 1.4g / cm3) into the solution 3 ), while accurately measuring 35ml of tetrabutyl titanate (equivalent to 0.1mol of titanium dioxide), slowly dropwise added to the solution, and kept stirring at 90°C for about 2h until the solution became clear. Keeping the temperature and stirring conditions constant, a certain amount of ammonia water was added dropwise to the solution until the pH of the solution was 7.0.

[0035]Subsequently, 1.510g (0.005mol) of ammonium niobate oxalate hydrate and 0.5850g (0.005mol) of ammonium metavanadate were accurately weighed and ad...

Embodiment 2

[0042] A method for making a biosensor, comprising the steps of:

[0043] 1) Preparation of conductive metal-doped metal oxide nanoparticles.

[0044] First, weigh 2.10 g (0.01 mol) of citric acid monohydrate, add it to a beaker containing 100 ml of deionized water, and heat and stir at 60° C. until the citric acid is fully dissolved. Then add 3.3ml of concentrated nitric acid (mass fraction is about 65%, density is about 1.4g / cm2) into the solution 3 ), while accurately measuring 35ml of tetrabutyl titanate (equivalent to 0.1mol of titanium dioxide), slowly dropwise added to the solution, and kept stirring at 80°C for about 2h until the solution became clear. Keeping the temperature and stirring conditions constant, a certain amount of ammonia water was added dropwise to the solution until the pH of the solution was 7.5.

[0045] Subsequently, 1.510 g (0.005 mol) of ammonium niobate oxalate hydrate was accurately weighed and added to the solution, stirred at 80° C. to fully...

Embodiment 3

[0051] A method for making a biosensor, comprising the steps of:

[0052] 1) Preparation of conductive metal-doped metal oxide nanoparticles.

[0053] First, weigh 0.186g (0.0005mol) of disodium ethylenediaminetetraacetate (EDTA), add it into a beaker containing 11ml of deionized water, and heat and stir at 100°C until disodium ethylenediaminetetraacetate is fully dissolved. Then add 0.22ml of concentrated nitric acid into the solution, and at the same time accurately measure 35ml of tetrabutyl titanate (equivalent to 0.1mol of titanium dioxide), slowly drop into the solution, and keep stirring at 100°C for about 1 hour until the solution becomes clear . Keeping the temperature and stirring conditions constant, a certain amount of ammonia water was added dropwise to the solution until the pH of the solution was 8.0.

[0054] Subsequently, 0.06 g (0.0005 mol) of ammonium metavanadate was accurately weighed and added to the solution, and stirred at a temperature of 100° C. to ...

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Abstract

The invention discloses a biosensor and a manufacturing method for the same, and belongs to the technical field of sensor detection. The biosensor comprises an electrode, wherein a composite film is arranged on the surface of the electrode, and comprises a biomolecular probe and conductive metal doped metal oxide nanoparticles; metal oxide has semiconductor properties; the molar ratio of conductive metal to the metal oxide is 0.5 to 10 percent. According to the biosensor and the manufacturing method for the same, the surface of the electrode is wrapped with the composite film comprising the conductive metal doped metal oxide nanoparticles and the biomolecular probe, the nanoparticles are introduced into the construction of a biosensitive interface by utilizing the characteristics of surface effects, quantum size effects, dielectric confinement effects and the like of the nanoparticles, and the conductive metal is doped into the metal oxide nanoparticles, so that the electron transfer efficiency of the composite film is improved, an oxidation-reduction electrochemical signal is enhanced, the sensitivity of the sensor is improved, and the limit of detection is reduced.

Description

technical field [0001] The invention relates to the technical field of sensor detection, in particular to a biosensor and a manufacturing method thereof. Background technique [0002] Biosensors are the product of the development and intersection of multiple disciplines that combine life sciences, analytical chemistry, materials science, information science and technology, and emerging nanoscience and technology. It can quickly analyze and track a variety of life and chemical substances, and has the characteristics of high selectivity, fast analysis speed, high sensitivity, low cost, online monitoring and even in vivo analysis in complex systems. So far, it has been gradually promoted and applied in clinical diagnosis, food analysis, environmental protection, biotechnology, biochip, bionics and other fields. [0003] At present, most of the preparation of electrochemical biosensor electrodes is to use bare electrodes such as gold, platinum, silver, carbon, glassy carbon, et...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 刘付建李核郭新东梁德沛陈纪文沈宏林王娜李衍春谭婉琪周桂萍冯艳陈满英陈卓梅叶淑贞
Owner GUANGDONG TESTING INST OF PROD QUALITY SUPERVISION
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