Separation and purification process for producing branched chain amino acid by using fermentation method

Abstract

The invention discloses a separation and purification process for producing branched chain amino acid by using a fermentation method. The process comprises the following steps: 1) preparing a rough product; 2) preparing branched chain amino acid salt by using a hydrochloric acid technique; 3) desalting to purify a multi-membrane combined system. As a rough fermentation finished product is adopted to prepare hydrochloride firstly, secondly, other amino acids are separated and purified, and subsequently the multi-membrane combination technique is adopted to replace an ion exchange technique, the acid and alkali consumption is reduced, the wastewater emission is reduced, and clean production, energy conservation and emission reduction are achieved; a fermentation liquid is partially desalted by using an electrodialysis technique in the multi-membrane combination technique, and the salt is removed by coupling the electrodialysis technique with the ion exchange technique, the problems of use amount of ion exchange resin recycled acid and alkali and salt contamination are effectively solved, and the water consumption is reduced.

Description

technical field [0001] The invention relates to a separation and purification process for producing branched-chain amino acids by fermentation. Background technique [0002] Domestic production of branched-chain amino acids is mainly based on fermentation technology. At present, the problems existing in fermentation production mainly include low acid production level of strains, high content of miscellaneous acids, and other amino acids produced by fermentation metabolism, especially neutral amino acids such as leucine, its molecular weight and chemical isoelectric point, and branched chain amino acids. It is very close to the extraction, and the ion exchange process is generally used to separate the impurity acids, and the process is complicated. The main disadvantage is that the ion exchange resin needs to be regenerated and eluted, and a large amount of acid and alkali will be used in the regeneration process, which makes the product purity low. And the yield is low; a l...

AI Technical Summary

Problems solved by technology

At present, the problems existing in fermentation production mainly include low acid production level of strains, high content of miscellaneous acids, and other amino acids produced by fermentation metabolism, especially neutral amino acids such as leucine, its molecular weight and chemical isoelectric point, and branched chain amino acids. It is very close to the extraction, and the ion exchange process is generally used to separate the impurity acids, and the process is complicated. The main disadvantage is that the ion exchange resin needs to be regenerated and eluted, and a large amount of acid and alkali will be used in the regeneration process, which makes the product purity low. And the yield is low; a large amount of ammonium nitrogen waste liquid will be produced after elution and regeneration, which will not only cost a lot of money due to the consumption of a large amount of acid and alkali, but also cause great pollution to the environment. consume a lot of water

Backward production technology limits scale production

Because the content of other amino acids in the product is difficult to solve, domestic high-end API products cannot meet the EU standards, so they cannot enter the international market to participate in the competition of APIs