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Preparing method of polyurethane-keratin composite nano fiber film

A technology of composite nanofibers and keratin, applied in the field of preparation of nanofiber membranes, can solve the problems of no biological activity, lack of cell recognition signal sites, unfavorable tissue cell adhesion and growth, etc., and achieve good toughness and mechanical strength. Effect

Inactive Publication Date: 2014-08-20
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the polyurethane material itself has no biological activity and lacks cell recognition signal sites, which is not conducive to the adhesion and growth of tissue cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The keratin was extracted from wool by reduction method. The specific method was: weigh 5g of wool, use petroleum ether as solvent to remove the oil on the surface of wool by Soxhlet extraction, then wash the wool with ethanol and air-dry it. Add the above-mentioned wool into 100ml, 1mol / l mercaptoethanol, adjust the pH to about 10.0 with NaOH, and then react at 40°C for 12h. After the reaction was finished, the reaction solution was collected (referred to as filtrate 1), and the wool solid remaining in the reaction was collected by filtration, and continued to be treated with 100ml, 0.1mol / l Tris lye at 40°C for 2h, and then filtered to remove the solid. The filtrate was collected (designated as filtrate 2). .The filtrates 1 and 2 were combined, centrifuged at 6000rpm for 5 minutes, and then dialyzed with a dialysis bag with a molecular weight cut-off of 40,000 for 36 hours. Finally, the dialysate was added to liquid nitrogen for quick freezing, and then dried in a fre...

Embodiment 2

[0029] Using human hair as raw material to extract keratin by reduction method, the specific method is: weigh 5g of human hair, use petroleum ether as solvent to remove human hair surface oil by Soxhlet extraction, then wash human hair with ethanol and air-dry. Add the above-mentioned human hair into a mixed solution of 200ml of water and 5g of sodium metabisulfite, adjust the pH to about 10.0 with NaOH, and then react at 60°C for 5h. After the reaction, collect the reaction solution (referred to as filtrate 1), and collect the remaining human hair solid by filtration at the same time, continue to treat it with 100ml, 0.1mol / l Tris lye at 40°C for 2h, and then filter to remove the solid , collect the filtrate (referred to as filtrate 2). .The filtrates 1 and 2 were combined, centrifuged at 6000rpm for 5 minutes, and then dialyzed with a dialysis bag with a molecular weight cut-off of 8,000 for 36 hours. Finally, the dialysate was added to liquid nitrogen for quick freezing, an...

Embodiment 3

[0032]The keratin is extracted from wool by oxidation method. The specific method is: weigh 5g of wool, use petroleum ether as solvent to remove the oil on the surface of wool by Soxhlet extraction, then wash the wool with ethanol and air-dry it. 100ml of deionized water and 5ml of 30% hydrogen peroxide were added to the wool, and then reacted at 100°C for 2h. After the reaction was finished, the reaction solution was collected (referred to as filtrate 1), and the wool solid remaining in the reaction was collected by filtration, and continued to be treated with 100ml, 0.1mol / l Tris lye at 40°C for 2h, and then filtered to remove the solid. The filtrate was collected (designated as filtrate 2). .The filtrates 1 and 2 were combined, centrifuged at 6000rpm for 5 minutes, and then dialyzed with a dialysis bag with a molecular weight cut-off of 8,000 for 36 hours. Finally, the dialysate was added to liquid nitrogen for quick freezing, and then dried in a freeze dryer to obtain dry ...

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Abstract

The invention relates to a preparing method of a polyurethane-keratin composite nano fiber film. The preparing method comprises the steps that polyurethane is dissolved in a solvent, dissolution by stirring is carried out, and a polyurethane solution is obtained; keratin is dissolved in a solvent, dissolution by stirring is carried out, and a keratin solution is obtained; and the polyurethane solution and the keratin solution are mixed according to the weight ratio of 95 : 5-5 : 95, blending by stirring is carried out, a polyurethane-keratin spinning solution is obtained, then electrostatic spinning is carried out, nano filaments are received by aluminum foil or cloth, and the polyurethane-keratin composite nano fiber film is obtained. According to the polyurethane-keratin composite nano fiber film, the good biological activity of the keratin is maintained, the good tenacity and the good mechanical strength of the polyurethane are achieved, and the polyurethane-keratin composite nano fiber film is expected to be used in fields such as surgical dressing.

Description

technical field [0001] The invention belongs to the field of preparation of nanofiber membranes, in particular to a preparation method of polyurethane-keratin composite nanofiber membranes. Background technique [0002] Keratin is an insoluble fibrous animal protein, a structural protein of ectodermal cells, widely present in animal skin and skin appendages, such as hair, hooves, shells, claws, horns, scales, etc. A large number of studies in recent years have shown that keratin is a high-quality biomedical material with good biocompatibility and not rejected by the body's immune system, and has broad application prospects. Most prominently, the amino acid sequence of keratin derived from wool and other sources was found to contain the same Arg-Gly-Asp (RGD) tripeptide sequence. This tripeptide sequence is recognized as an effective binding site for cell binding in the extracellular matrix, and has the function of promoting cell adsorption. Therefore, a large number of bas...

Claims

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Application Information

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IPC IPC(8): D04H1/4382D04H1/728A61L15/32A61L15/44A61L15/42
Inventor 杨光王夏辉
Owner DONGHUA UNIV
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