Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Method for preparing high-activity plasmin from bean dregs

A plasmin, high activity technology, applied in the field of biochemistry, can solve the problem of no related reports and so on

Inactive Publication Date: 2014-08-27
NORTHEAST AGRICULTURAL UNIVERSITY
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research on microbial mixed culture, especially the research direction of using mixed culture to produce enzymes, is generally based on screening compatible strains, and then testing the ability of enzyme production under different mixing conditions, and research on its mechanism and other aspects. There is no relevant report yet

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing high-activity plasmin from bean dregs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] After the bean dregs are dried, under the condition of the feed airflow of 0.6MPa and the crushing airflow of 0.6MPa, the QLM flat supersonic airflow milling system is used for ultrafine pulverization, and the water content of the ultrafine pulverized bean dregs is adjusted, and then sterilized , after cooling, under the conditions of fermentation temperature of 32°C and fermentation pH of 7.5, insert 6% Aspergillus niger and 8% Aspergillus oryzae for solid-state fermentation for 30 hours to obtain the fermentation product. Under the conditions of ultrasonic power of 60W and extraction temperature of 30°C , disperse the fermentation product in normal saline for ultrasonic assisted leaching for 3 hours, centrifuge after leaching to obtain a supernatant, add ammonium sulfate solution with a saturation of 60% to the supernatant for salting out, and perform dialysis after salting out Concentration, DEAE-Sepharose FastFlow anion exchange chromatography and Sephadex G-75 gel f...

Embodiment 2

[0022] After the bean dregs are dried, under the condition of the feed airflow of 0.6MPa and the crushing airflow of 0.6MPa, the QLM flat supersonic airflow milling system is used for ultrafine pulverization, and the water content of the ultrafine pulverized bean dregs is adjusted, and then sterilized , after cooling, under the conditions of fermentation temperature of 32°C and fermentation pH of 7.5, insert 6% Aspergillus niger and 8% Aspergillus oryzae for solid-state fermentation for 35 hours to obtain the fermentation product. Under the conditions of ultrasonic power of 80W and extraction temperature of 30°C , disperse the fermentation product in normal saline for ultrasonic assisted leaching for 2 hours, centrifuge after leaching to obtain a supernatant, add ammonium sulfate solution with a saturation of 70% to the supernatant for salting out, and perform dialysis after salting out Concentration, DEAE-Sepharose FastFlow anion exchange chromatography and Sephadex G-75 gel f...

Embodiment 3

[0024] After the bean dregs are dried, under the condition of the feed airflow of 0.6MPa and the crushing airflow of 0.6MPa, the QLM flat supersonic airflow milling system is used for ultrafine pulverization, and the water content of the ultrafine pulverized bean dregs is adjusted, and then sterilized After cooling, at a fermentation temperature of 32°C and a fermentation pH of 7.5, insert 6% Aspergillus niger and 8% Aspergillus oryzae for solid-state fermentation for 25 hours to obtain a fermentation product. Under the conditions of ultrasonic power of 60W and extraction temperature of 30°C , disperse the fermentation product in normal saline and carry out ultrasonic-assisted leaching for 4 hours, centrifuge after leaching to obtain a supernatant, add ammonium sulfate solution with a saturation of 60% to the supernatant for salting out, and perform dialysis after salting out Concentration, DEAE-Sepharose FastFlow anion exchange chromatography and Sephadex G-75 gel filtration c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for preparing high-activity plasmin from bean dregs, which belongs to the field of biochemistry. The method comprises the following steps: (1) after bean dregs are dried, carrying out superfine grinding on the bean dregs; (2) carrying out moisture adjustment on the bean dregs subjected to superfine grinding, sterilizing and cooling the obtained product, and inoculating aspergillus niger and aspergillus oryzae into the obtained product to carry out solid state fermentation, so that a fermentation product is obtained; (3) dispersing the fermentation product into physiological saline to carry out ultrasonic assisted extraction, and after extraction is completed, carrying out centrifugal separation on the obtained product so as to obtain liquid supernatant; and (4) carrying out ammonium sulfate salting-out on the liquid supernatant, then carrying out dialysis and concentration, anion exchange chromatography and gel filtration chromatography on the obtained object, and then carrying out vacuum concentration and freeze-drying on the obtained product, so that plasmin is obtained. The method is simple in operation, low in cost and high in production efficiency; the prepared plasmin is a single component, and the apparent molecular weight of the plasmin is 30 KDa, therefore, the plasmin is a typical serine protease; and the plasmin is high in activity and purity.

Description

technical field [0001] The invention belongs to the field of biochemistry, and in particular relates to a method for preparing high-activity fibrinolytic enzyme from bean dregs. Background technique [0002] Thrombosis is a common and frequently-occurring disease that seriously endangers human health, and is one of the diseases that threaten the greatest death. With the development of the aging trend of my country's population, cardiovascular and cerebrovascular diseases will increasingly threaten our health. Therefore Therefore, the development of highly effective thrombolytic drugs has become an urgent clinical requirement. Natural thrombolytic substances have been found in animals, plants, and microorganisms, and microorganisms are an important source of fibrinolytic substances. At present, fibrinolytic enzymes have been found in bacteria, fungi, and algae. The sources include: Bacillus, Streptococcus, Aspergillus, Scorchella. At present, almost all the researches on mic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/68C12R1/685C12R1/69
CPCC12N9/62C12Y304/24072
Inventor 吴非赵城彬孙楚李秋姚子鹏周媛媛刘瑶董兴叶盛慧刘金阳
Owner NORTHEAST AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com