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Electrochemical sensor for detecting lead as well as preparation method and application thereof

An electrochemical and sensor technology, applied in the field of electrochemical sensors for detecting lead and its preparation, can solve the problems of unstable fixation, affecting DNA activity, and easy use of environmentally harmful substances, so as to improve sensitivity, improve detection performance, and improve The effect of sensitivity

Inactive Publication Date: 2014-09-03
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods all have defects such as weak fixation, the use of various affinity substances in fixation to affect the activity of DNA, and easy use of substances harmful to the environment.

Method used

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  • Electrochemical sensor for detecting lead as well as preparation method and application thereof
  • Electrochemical sensor for detecting lead as well as preparation method and application thereof
  • Electrochemical sensor for detecting lead as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] see figure 1 , an electrochemical sensor for the detection of lead ions, comprising a glassy carbon electrode used as a working electrode in a three-electrode system, an aptamer probe, and methylene blue. The surface of the detection end of the glassy carbon electrode is modified with multi-walled carbon nanotubes, nano-gold particles are deposited on the multi-walled carbon nanotubes, and thiol-modified capture probes are connected to the nano-gold particles. When the electrochemical sensor is used to detect lead ions, the aptamer probe and the sulfhydryl-modified capture probe form a double-stranded structure through complementary pairing, and methylene blue (0.1 mM concentration) is embedded in the sulfhydryl-modified capture probe and aptamer probe. in the double-stranded structure formed.

[0041] The concentration of methylene blue can also be 0.1-0.5 mM.

[0042] In the present invention, the capture probe and the aptamer probe can be any amino acid sequence th...

Embodiment 2

[0048] The preparation method of the electrochemical sensor of embodiment 1.

[0049] Polish the surface of the glassy carbon electrode, then wash the surface of the glassy carbon electrode with water, then use nitric acid, acetone, and water to ultrasonically clean it, and finally use a concentration of 10mM Tris-HCl buffer solution (Tris-HCl buffer solution contains 1.0M KCl ) rinsed, dried naturally, and then used for the preparation of electrochemical sensors, the specific preparation method is:

[0050] S1. Carboxylate the multi-walled carbon nanotubes to obtain carboxylated multi-walled carbon nanotubes; the specific steps are:

[0051] The multi-walled carbon nanotubes are immersed in a mixed solution with a volume ratio of 1:3 of hydrogen peroxide and concentrated sulfuric acid (the mass fraction of concentrated sulfuric acid is 98%) (the volume ratio of hydrogen peroxide and concentrated sulfuric acid can also be 1:2 ~4), ultrasonication at a temperature of 50°C for ...

Embodiment 3

[0060] The application of the electrochemical sensor of embodiment 1 in detecting lead ion, concrete detection method is:

[0061] Add the aptamer probe dropwise to the reaction end surface of the glassy carbon electrode of the electrochemical sensor, react at 37°C for 60 minutes, and then drop the methylene blue with a concentration of 0.1mM (the concentration of methylene blue can be 0.1-0.5mM) on the glassy carbon electrode The surface of the reaction end was reacted at 37°C for 20 minutes; then the concentration of lead ions was respectively adjusted to 5.0×10 -11 M~1.0×10 -14 The test solution of M was added dropwise on the surface of the glassy carbon electrode, and after 30 minutes of reaction, it was connected to the electrolytic cell of the three-electrode system, and the current value was detected with Tris-HCl with a pH of 7.4 as the electrolyte solution.

[0062] Figure 4 is the concentration of lead ions are 0M(a), 1.0×10 -14 M(b), 5.0×10 -14 M(c), 1.0×10 -1...

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Abstract

The invention relates to an electrochemical sensor for detecting lead. The electrochemical sensor comprises a glassy carbon electrode, wherein the glassy carbon electrode is utilized as a working electrode in a tri-electrode system, the surface of a detection end of the glassy cabon electrode is modified by a multi-walled carbon nanotube, nano golden particles are deposited on the multi-walled carbon nanotube, and a sulfydryl-modified capturing probe is connected with the nano golden particles; the electrochemical sensor further comprises an aptamer probe, wherein the aptamer probe and the sulfydryl-modified capturing probe are complementally paired to form a double-chain structure. According to the electrochemical sensor for detecting the lead, the aptamer probe is utilized to cover lead ions so as to be separated from the capturing probe, and during the separation, an electrochemical signal to be detected is generated, so that the purpose of detecting the content of the lead ions in the water body is achieved; the electrochemical sensor has the advantages of high sensitivity, excellent selectivity and stability, and the like.

Description

technical field [0001] The invention relates to the technical field of electrochemical sensors, in particular to an electrochemical sensor for detecting lead and its preparation method and application. Background technique [0002] At present, the methods for determining pollutants in the environment mainly include chromatography, ultraviolet spectroscopy, synchronous fluorescence spectroscopy, spectrophotometry, derivative photometry, and flow injection analysis. These methods have defects such as complex pretreatment, long time consumption, large sample matrix effect, and long analysis cycle. They have high requirements on the operation level of instruments and staff, and it is difficult to popularize and apply them in small and medium-sized enterprises. For example, when using spectrophotometry to detect pollutants, due to the requirements for substrate turbidity and the influence of light-interfering substances, its accuracy and scope of use are limited; while liquid and...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/26
Inventor 竺园曾光明章毅汤琳陈俊程敏郭远袁玉洁
Owner HUNAN UNIV
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