A Method for Degrading Straw Lignin by Cerexonas cerevisiae

A technology of straw lignin and cerethroid, applied in the field of bioengineering, can solve the problems of large consumption, unsatisfactory treatment effect, large space occupation and the like

Active Publication Date: 2017-11-14
STATE GRID CORP OF CHINA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Steam explosion is to use high-pressure steam to infiltrate the interior of the fiber and release it from the closed space in the form of air flow. The fiber undergoes a certain mechanical fracture, and most of the hemicellulose and a small amount of lignin and cellulose in the raw material are degraded and dissolved. The composition of the method is relatively high, large-scale equipment is required, and lignin needs to be treated; the acid treatment method uses sulfuric acid to hydrolyze glycosidic bonds, ester bonds, etc., so that the network structure of corn stalk fibers is loose, but this method consumes a large amount of organic acid or Inorganic acid and lignin still need to be further processed and are not suitable for large-scale industrial production
Alkali treatment and ammonia water treatment use lignin to dissolve in alkaline solution, and there are also environmental pollution problems like acid treatment
This is due to the fact that these methods generally have problems such as difficult control of reaction conditions, high reaction temperature, long treatment time, large space occupation, unsatisfactory treatment effect, and inability to remove lignocellulose.

Method used

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  • A Method for Degrading Straw Lignin by Cerexonas cerevisiae
  • A Method for Degrading Straw Lignin by Cerexonas cerevisiae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Production of bacteria on test tube slant

[0026] Insert the strain of Cerexoides annulus into the newly configured potato sucrose medium ("Industrial Microbiology Experimental Technology Manual" edited by Zhuge Jian and Wang Zhengxiang, 1994, page 367), at 31 ° C, and the incubation time is 50 hours; 4 Store at ℃ for later use.

[0027] 2. Production of strains cultured in liquid shake flasks

[0028] Accurately weigh 5 grams of corncob powder, 0.1 grams of ammonium tartrate, 0.2 grams of benzyl alcohol, 0.2 grams of magnesium sulfate, 800.5 grams of Tween, 2 grams of potassium dihydrogen phosphate, 3 grams of phthalic acid buffer, 1000 mL of water, pH 5, Pack in 250mL triangular flasks, 50 grams per bottle, 20 bottles in total, and sterilize at 120°C for 40 minutes; / min, cultivated for 72 hours;

[0029] 3. Production of first-level liquid strains

[0030] Accurately weigh 50 grams of corn cob powder, 1 gram of ammonium tartrate, 2 grams of benzyl alcohol, 2 ...

Embodiment 2

[0034] 1. Production of bacteria on test tube slant

[0035] Inject the strain of Cerephoides cerevisiae into the newly configured potato sucrose medium ("Industrial Microbiology Experimental Technology Manual" edited by Zhuge Jian and Wang Zhengxiang, 1994, page 367), at 41 ° C, and the incubation time is 100 hours; 7 Store at ℃ for later use.

[0036] 2. Production of strains cultured in liquid shake flasks

[0037] Accurately weigh 150 grams of corncob powder, 250 grams of ammonium tartrate, 15 grams of benzyl alcohol, 0.4 grams of magnesium sulfate, 804 grams of Tween, 5 grams of potassium dihydrogen phosphate, 20 grams of phthalic acid buffer, 10 L of water, pH 6, divide Packed in 250mL triangular flasks, 100 grams per bottle, 100 bottles in total, sterilized at 130°C for 30 minutes; after cooling, each bottle was inserted with a ring of Ceresoides sp. preserved at 7°C, at 41°C, 150 rpm minutes, cultivated for 50 hours;

[0038] 3. Production of first-level liquid stra...

Embodiment 3

[0043] 1. Production of bacteria on test tube slant

[0044] Introduce the strain of Cerexoides annulus into the newly configured potato sucrose medium ("Industrial Microbiology Experimental Technology Manual" edited by Zhuge Jian and Wang Zhengxiang, 1994, page 367), at 58°C, and the culture time is 144 hours; 10 Store at ℃ for later use.

[0045] 2. Production of strains cultured in liquid shake flasks

[0046] Accurately weigh 300 grams of corncob powder, 50 grams of ammonium tartrate, 30 grams of benzyl alcohol, 10 grams of magnesium sulfate, 100 grams of Tween, 90 grams of potassium dihydrogen phosphate, 180 grams of phthalic acid buffer, 10 L of water, pH7. 5. Divide into 250mL triangular flasks, 120mL per bottle, 80 bottles in total, sterilize at 140°C for 20 minutes; rpm, cultivated for 72 hours;

[0047] 3. Production of first-level liquid strains

[0048] Accurately weigh 1,500 grams of corncob powder, 250 grams of ammonium tartrate, 150 grams of benzyl alcohol, ...

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PUM

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Abstract

The invention provides a method for degrading straw lignin by using Ceriporiopsis subvermispora, which belongs to the field of bioengineering. According to the method, Ceriporiopsis subvermispora is used as an original strain and straw lignin is degraded through solid state fermentation. Production process flow of the method comprises the following steps: pretreatment procedures consisting of crushing, pre-wetting, etc.; liquid shake-flask culture of Ceriporiopsis subvermispora; primary seed culture; mixing with straw and solid state fermentation; and degradation of lignin in the straw. The fermentation technology is simple and convenient, poses no pollution to the environment and wastes no resource. With the method, the degradation rate of lignin is 70 to 96%, and straw with degraded lignin can be used for preparation of fuel ethanol.

Description

technical field [0001] The invention relates to the field of bioengineering, and in particular refers to a process for degrading lignin in straws by the fungus Acetophagous cerevisiae. Background technique [0002] Energy is the driving force for the survival and sustainable development of human society. At present, 80% of the energy used in the world is non-renewable fossil fuels. However, these fuel resources are limited and will be exhausted sooner or later. In addition, human beings use fossil fuels. Emit large amounts of carbon dioxide and sulfur dioxide gases into the atmosphere, causing global warming. Biomass energy is a substitute for fossil energy, and it has the advantages that fossil energy cannot replace, that is, biomass energy is clean energy, and resources are not limited. Therefore, the development of biomass energy is a key measure to solve the energy crisis. [0003] my country is a large agricultural country, with an annual output of more than 600 millio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D21C5/00C12N1/14C12P7/10C12P19/14C12R1/645
CPCY02E50/10
Inventor 高苏杰吴毅赵鹏翔赵承宇李强赵正凯
Owner STATE GRID CORP OF CHINA
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