Freshwater compound microorganism substrate modifier

A technology of compound microorganisms and primers, applied in biological water/sewage treatment, water/sludge/sewage treatment, chemical instruments and methods, etc., can solve the problem of low survival rate of effective viable bacteria, unstable product quality, use Unsatisfactory effect and other problems, to achieve the effect of stable product quality, stable use effect, and avoiding secondary pollution

Active Publication Date: 2014-12-03
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a freshwater compound microbial bottom modifier for the deficiencies of the above-mentioned prior art. The organic matter is flocculated and settled, and the strong decomposition of aerobic bacteria, facultative anaerobic bacteria and strict anaerobic bacteria in the product is used to degrade the organic matter together, so as to achieve the effect of improving the bottom quality of freshwater aquaculture water, and has the advantages of simple production process and low cost. And the characteristics of low energy consumption, high number of effective viable bacteria, long shelf life, strong adaptability, and easy mass production solve the problems of unstable product quality, unsatisfactory use effect, and effective activity of substrate improvers for aquatic products in the market. Problems such as low bacterial survival rate and high production cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Preparation of Clostridium butyricum liquid seed medium: add 15g glucose, 8g peptone, 5g yeast powder, 1g corn steep liquor, 1.5g ammonium citrate, 4g anhydrous sodium acetate and 0.7g K to 1000ml water 2 HPO 4 Mix well, and the pH is 7.0.

[0023] Preparation of solid medium: 70% wheat bran, 10% corn flour, 10% rice flour, 6.5% light calcium carbonate, 1.5% glucose, 1.7% bean cake powder, KH 2 PO 4 0.1%, MgSO 4 .7H 2 O 0.1% and sodium dehydroacetate 0.1%, mix well, and adjust the water content to 52%.

[0024] Preparation of Bacillus natto powder: inoculate one ring of Bacillus natto slant strain cells into the nutrient medium of liquid expansion broth, and vibrate on a shaker for 20 hours at 37°C and 160r / min to obtain Bacillus natto liquid seed, the liquid seed cell concentration is 4.5×109 cfu / mL; then put the liquid seeds of Bacillus natto into the solid medium according to the inoculation weight of 25%, mix them well, place them in a shallow fermentation pan,...

Embodiment 2

[0029] Preparation of Clostridium butyricum liquid seed medium: add 10g glucose, 5g peptone, 2g yeast powder, 0.6g corn steep liquor, 0.8g ammonium citrate, 2.5g anhydrous sodium acetate and 0.4g K to 1000ml water 2 HPO 4 Mix well, and the pH is 7.0.

[0030] Preparation of solid medium: take 65% of wheat bran, 15% of corn flour, 7.6% of rice flour, 8% of light calcium carbonate, 2% of glucose, 2% of bean cake powder, KH 2 PO 4 0.2%, MgSO 4 .7H 2 O 0.15% and sodium dehydroacetate 0.05%, mix well, and adjust the water content to 50%.

[0031] Preparation of Bacillus natto bacteria powder: inoculate one ring of Bacillus natto bacteria cells into the liquid expansion broth nutrient medium, and shake it on a shaker for 24 hours at 35°C and 180r / min to prepare Bacillus natto liquid seed, the liquid seed cell concentration is 9.3×10 8 cfu / mL; then put the liquid seeds of Bacillus natto into the solid medium according to the inoculation weight of 20%, mix them well, place them ...

Embodiment 3

[0036] Preparation of Clostridium butyricum liquid seed medium: add 12g glucose, 6g peptone, 4g yeast powder, 0.8g corn steep liquor, 1.2g ammonium citrate, 3.2g anhydrous sodium acetate and 0.5g K to 1000ml water 2 HPO 4 Mix well, and the pH is 7.0.

[0037] Preparation of solid medium: 75% wheat bran, 12.07% corn flour, 5% rice flour, 5% light calcium carbonate, 1.0% glucose, 1.5% bean cake powder, KH 2 PO 4 0.3%, MgSO 4 .7H 2 O 0.05% and sodium dehydroacetate 0.08%, mix evenly, and adjust the water content to 55%.

[0038] Preparation of Bacillus natto powder: inoculate one ring of Bacillus natto slant strain cells into the nutrient medium of liquid expansion broth, and culture it on a shaking table for 22 hours under the conditions of 36°C and 170r / min. Bacillus natto liquid seed, the liquid seed cell concentration is 5.32×10 9 cfu / mL; then put the liquid seeds of Bacillus natto into the solid medium according to the inoculation weight of 22%, mix them well, place th...

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PUM

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Abstract

The invention discloses a freshwater compound microorganism substrate modifier, and belongs to the field of biotechnology in freshwater aquaculture. The substrate modifier is formed by mixing and uniformly stirring bacillus natto powder, lactic acid bacteria powder, clostridia butyricum powder, a biological flocculant and an oxygen producer. According to the substrate modifier disclosed by the invention, after organic pollutants in aquatic water are flocculated to form bottom mud by virtue of flocculating effects of the biological flocculant, organic flocculate in the bottom mud is decomposed by virtue of strong decomposition of aerobic bacteria, facultative anaerobe and strict anaerobe, so that the quality of the bottom mud of the freshwater aquatic water is improved; and the freshwater compound microorganism substrate modifier has the characteristics of biodegradability, safety, efficiency, no toxicity and no secondary pollution.

Description

technical field [0001] The invention belongs to the field of biotechnology in freshwater aquaculture, and in particular relates to a freshwater composite microbial bottom modifier. Background technique [0002] With the continuous increase of aquaculture density, the aquaculture mode of production is maintained at the cost of consuming a large amount of high-protein feed and polluting the pond itself and the surrounding environment, which destroys the original ecological balance of the pond. At the same time, in the aquaculture process, the aquaculture residue Bait, feces, dead animals (plants), and pesticides, disinfectants, antibiotics and other chemicals are deposited at the bottom of the pool to form black sludge. Sludge is rich in organic matter, and anaerobic microorganisms dominate, seriously destroying the micro-ecological environment of the substrate, causing various toxic and harmful substances to deteriorate the substrate, thereby endangering farmed fish. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34
Inventor 肖调义兰时乐周芳如李玥历大伟王红权
Owner HUNAN AGRICULTURAL UNIV
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