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Endophytic bacteria bacillus atrophaeus bacterial strain of forage in alpine grassland, microbial agent as well as preparation method and application of microbial agent

A technology of Bacillus fructosa and microbial agent, applied in the field of microorganisms, can solve the problems of poor environmental protection, drug resistance and safety of chemical pesticides and chemical fertilizers

Inactive Publication Date: 2014-12-10
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of endophytic bacterium Bacillus fumeus strain aiming at the defects of poor environmental protection, easy to produce drug resistance and poor safety of chemical pesticides and chemical fertilizers in the above-mentioned prior art

Method used

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  • Endophytic bacteria bacillus atrophaeus bacterial strain of forage in alpine grassland, microbial agent as well as preparation method and application of microbial agent
  • Endophytic bacteria bacillus atrophaeus bacterial strain of forage in alpine grassland, microbial agent as well as preparation method and application of microbial agent
  • Endophytic bacteria bacillus atrophaeus bacterial strain of forage in alpine grassland, microbial agent as well as preparation method and application of microbial agent

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Effect test

Embodiment 1

[0060] An endophytic bacterium, Bacillus atrophaeus strain, named as Bacillus atrophaeus strain QLY001, is preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms, and the preservation time is March 10, 2014, and the preservation number is It is CGMCC No.8904; the concentration of secreting IAA in the King's medium without tryptophan is 2.6mg / L in the described Bacillus atrophaeus (Bacillus atrophaeus) bacterial strain QLY001, and in King's medium containing 100mg / L tryptophan The concentration of secreted IAA in Shi's medium was 3.42mg / L.

[0061] The application of the above-mentioned endophytic bacterium Bacillus fuecata strain in inhibiting pathogenic bacteria, said pathogenic bacteria include potato brown rot fungus (Stysanus stemonitis), potato gangrene fungus (Phoma foveata), wheat root rot fungus (Bipolaris sorokiniana), tomato early blight Alternaria solani, Colletotrichum coccodes, Fusarium avenaceum, Fusarium solani and ...

Embodiment 2

[0064] A kind of microbial bacterial agent, its active component is the thalline of above-mentioned bacillus atrophaeus (Bacillus atrophaeus) bacterial strain QLY001, wherein, contain Bacillus atrophaeus (Bacillus atrophaeus) bacterial strain QLY001: 6% by weight percentage, the remaining Amount, the culture medium is made up of following material: ammonium chloride 8g, corn flour 13g, beef extract 4g, distilled water or deionized water 1000mL, the pH value of described culture medium is 6.6, the Bacillus atrophaeus (Bacillus atrophaeus) bacterial strain QLY001 The number of live bacteria is 1.82×10 10 CFU / mL.

[0065] The preparation method of the above-mentioned microbial bacterial agent is to insert Bacillus atrophaeus (Bacillus atrophaeus) bacterial strain QLY001 into the culture medium for fermentation, the insertion amount is 6%, the fermentation time is 33h, the fermentation temperature is 24°C, and the fermentation speed is 140r / min, the dissolved oxygen in fermentat...

Embodiment 3

[0067] A microbial inoculant whose active ingredient is the thalline of the above-mentioned Bacillus atrophaeus bacterial strain QLY001, wherein it contains Bacillus atrophaeus bacterial strain QLY001: 9% by weight percentage, and the remaining Amount, culture medium is made up of following material: ammonium chloride 12g, corn flour 17g, beef extract 5g, distilled water or deionized water 1000mL, the pH value of described culture medium is 7.0, the Bacillus atrophaeus (Bacillus atrophaeus) bacterial strain QLY001 The number of live bacteria is 3.5×10 10 CFU / mL.

[0068] The preparation method of the above-mentioned microbial bacterial agent is to insert Bacillus atrophaeus (Bacillus atrophaeus) bacterial strain QLY001 into the culture medium for fermentation, the insertion amount is 9%, the fermentation time is 36h, the fermentation temperature is 28 ℃, and the fermentation speed is 160r / min, the dissolved oxygen in fermentation is 65%. Microbial agents can be used to prep...

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Abstract

The invention discloses an endophytic bacteria bacillus atrophaeus bacterial strain of forage in alpine grassland, and also provides a microbial agent prepared by virtue of fermentation of the endophytic bacteria bacillus atrophaeus bacterial strain and application of the microbial agent. The endophytic bacteria bacillus atrophaeus bacterial strain is named a bacillus atrophaeus bacterial strain QLY001 with a preservation number: CGMCC No.8904. The bacillus atrophaeus bacterial strain QLY001 microbial agent has a bacteriostatic action, and can be used as a microbial pesticide to inhibit monilinia fructicola of potatoes, phoma exigua, wheat root rot, early blight pathogenic bacteria of tomatoes and colletotrichum coccodes, wherein a bacteriostatic ratio can reach over 60%. Moreover, the defects that the chemical pesticide in the prior art is poor in environmental protection, easily produces drug resistance and poor in safety are overcome to realize the advantages that the environmental protection is good, the drug resistant is not easy to produce and the safety is good.

Description

technical field [0001] The invention relates to the field of microbes, in particular to an endophytic bacterium Bacillus fumeus strain, a microbial agent and a preparation method and application thereof. Background technique [0002] With the development of society, people have higher and higher requirements for food. Based on the environmental pollution and pesticide residues of chemical pesticides, the biological control of plant diseases has become a research hotspot, especially the research on plant endophytic bacteria as antagonistic bacteria. received the attention of many scholars. Plant endophytic bacteria refer to bacteria that live in plants but do not cause any substantial damage to plants. They have biological functions such as nitrogen fixation, phosphorus dissolution, and IAA production. Especially, the antibacterial function is a powerful source of biocontrol bacteria. support. The biological functions of plant endophytes, such as nitrogen fixation, phosphor...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C05F11/08C12R1/07
Inventor 杨成德陈秀蓉薛莉畅涛
Owner GANSU AGRI UNIV
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