Endophytic bacteria bacillus atrophaeus bacterial strain of forage in alpine grassland, microbial agent as well as preparation method and application of microbial agent

A technology of Bacillus fructosa and microbial agent, applied in the field of microorganisms, can solve the problems of poor environmental protection, drug resistance and safety of chemical pesticides and chemical fertilizers

Inactive Publication Date: 2014-12-10
GANSU AGRI UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] The object of the present invention is to provide a kind of endophytic bacterium Bacillus fumeus strain aiming at the defects of poor enviro

Method used

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  • Endophytic bacteria bacillus atrophaeus bacterial strain of forage in alpine grassland, microbial agent as well as preparation method and application of microbial agent
  • Endophytic bacteria bacillus atrophaeus bacterial strain of forage in alpine grassland, microbial agent as well as preparation method and application of microbial agent
  • Endophytic bacteria bacillus atrophaeus bacterial strain of forage in alpine grassland, microbial agent as well as preparation method and application of microbial agent

Examples

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Example Embodiment

[0059] Example 1:

[0060] A strain of endophytic bacteria called Bacillus atrophaeus (Bacillus atrophaeus) strain QLY001. The preservation unit is the General Microbiology Center of China Microbial Culture Collection Management Committee. The preservation time is March 10, 2014. The preservation number is Is CGMCC No. 8904; the Bacillus atrophaeus strain QLY001 secretes IAA at a concentration of 2.6 mg / L in King’s medium without tryptophan, and in King’s medium containing 100 mg / L tryptophan. The concentration of secreted IAA in the culture medium is 3.42mg / L.

[0061] Application of the above-mentioned endophytic bacterium Bacillus seperum strain in inhibiting pathogens, the pathogens including potato brown rot (Stysanus stemonitis), potato gangrene (Phoma foveata), wheat root rot (Bipolaris sorokiniana), tomato early blight Pathogen (Alternaria solani), potato anthracnose (Colletotrichum coccodes), potato wilt (Fusarium avenaceum), cumin root rot (Fusarium solani) and potato dr...

Example Embodiment

[0063] Example 2:

[0064] A microbial inoculum, the active ingredient of which is the above-mentioned Bacillus atrophaeus strain QLY001, which contains Bacillus atrophaeus strain QLY001: 6% by weight, and the remaining medium The medium is composed of the following materials: 8g of ammonium chloride, 13g of corn flour, 4g of beef extract, 1000mL of distilled water or deionized water, the pH of the medium is 6.6, Bacillus atrophaeus (Bacillus atrophaeus) strain QLY001 The number of viable bacteria is 1.82×10 10 CFU / mL.

[0065] The preparation method of the above microbial inoculum is to insert Bacillus atrophaeus strain QLY001 into the culture medium for fermentation, the insert amount is 6%, the fermentation time is 33h, the fermentation temperature is 24℃, and the fermentation speed is 140r / min, the dissolved oxygen in fermentation is 55%. Microbial agents can be used to prepare pesticides or fertilizers.

Example Embodiment

[0066] Example 3:

[0067] A microbial agent, the active ingredient of which is the above-mentioned Bacillus atrophaeus strain QLY001, which contains Bacillus atrophaeus strain QLY001: 9% by weight, and the remaining medium The medium is composed of the following substances: 12g ammonium chloride, 17g corn flour, 5g beef extract, 1000mL distilled water or deionized water, the pH of the medium is 7.0, and the Bacillus atrophaeus strain QLY001 The number of viable bacteria is 3.5×10 10 CFU / mL.

[0068] The preparation method of the above microbial inoculum is to insert Bacillus atrophaeus strain QLY001 into the culture medium for fermentation, the insert amount is 9%, the fermentation time is 36h, the fermentation temperature is 28℃, and the fermentation speed is 160r / min, the dissolved oxygen in fermentation is 65%. Microbial agents can be used to prepare pesticides or fertilizers.

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Abstract

The invention discloses an endophytic bacteria bacillus atrophaeus bacterial strain of forage in alpine grassland, and also provides a microbial agent prepared by virtue of fermentation of the endophytic bacteria bacillus atrophaeus bacterial strain and application of the microbial agent. The endophytic bacteria bacillus atrophaeus bacterial strain is named a bacillus atrophaeus bacterial strain QLY001 with a preservation number: CGMCC No.8904. The bacillus atrophaeus bacterial strain QLY001 microbial agent has a bacteriostatic action, and can be used as a microbial pesticide to inhibit monilinia fructicola of potatoes, phoma exigua, wheat root rot, early blight pathogenic bacteria of tomatoes and colletotrichum coccodes, wherein a bacteriostatic ratio can reach over 60%. Moreover, the defects that the chemical pesticide in the prior art is poor in environmental protection, easily produces drug resistance and poor in safety are overcome to realize the advantages that the environmental protection is good, the drug resistant is not easy to produce and the safety is good.

Description

technical field [0001] The invention relates to the field of microbes, in particular to an endophytic bacterium Bacillus fumeus strain, a microbial agent and a preparation method and application thereof. Background technique [0002] With the development of society, people have higher and higher requirements for food. Based on the environmental pollution and pesticide residues of chemical pesticides, the biological control of plant diseases has become a research hotspot, especially the research on plant endophytic bacteria as antagonistic bacteria. received the attention of many scholars. Plant endophytic bacteria refer to bacteria that live in plants but do not cause any substantial damage to plants. They have biological functions such as nitrogen fixation, phosphorus dissolution, and IAA production. Especially, the antibacterial function is a powerful source of biocontrol bacteria. support. The biological functions of plant endophytes, such as nitrogen fixation, phosphor...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C05F11/08C12R1/07
Inventor 杨成德陈秀蓉薛莉畅涛
Owner GANSU AGRI UNIV
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