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A strain of Lactobacillus flexus and its application

A technology of Lactobacillus flexus and bacteria agent, which is applied in the field of microorganisms, can solve the problems of easy recurrence and achieve the effect of strong adhesion

Active Publication Date: 2017-04-12
北京益生苑生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Urogenital tract infection is a common and frequently-occurring disease among women, and is prone to recurrence. Although antibiotics have a good short-term effect, there is a 40% recurrence rate

Method used

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  • A strain of Lactobacillus flexus and its application
  • A strain of Lactobacillus flexus and its application
  • A strain of Lactobacillus flexus and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1. Isolation and identification of Lactobacillus campylobacter strains

[0031] 1. Isolation of Lactobacillus Campylobacter Strains

[0032] More than 200 samples of vaginal secretions from healthy women who were clinically detected without symptoms and signs of vaginitis were serially numbered and inoculated into MRS liquid medium and cultured anaerobicly at 37°C for 24 hours. For each sample, 5 single colonies were randomly selected and cultured in 2mL MRS liquid medium for 24 hours, and the colonies were numbered sequentially. Hundreds of isolated bacteria were identified, hydrogen peroxide production and adhesion to vaginal epithelial cells were tested. One strain of Lactobacillus with strong adhesion and hydrogen peroxide production was named IMC-12.

[0033] 2. Identification of Lactobacillus Campylobacter Strains

[0034] (1) Morphological identification

[0035] On the MRS Lactobacillus culture plate, the strain IMC-12 isolated in the above step 1 shows the col...

Embodiment 2

[0048] Example 2. Functional analysis of Lactobacillus crispatus IMC-12

[0049] 1. Lactobacillus crispatus (Lactobacillus crispatus) IMC-12 produces H 2 O 2 Detection

[0050] (1) H 2 O 2 The preparation of the standard curve: First, use a solution of piperazine-N, N_-Bis 2-ethanesulfonic acid (PIPES) with a concentration of 100mmol / L to set the volume percentage to 30 %H 2 O 2 Aqueous solution (equivalent to 9.128mol / L) stock solution is diluted to 1mol / L, and then 100mmol / LPIPES aqueous solution to the above 1mol / L H 2 O 2 Dilute to 20μmol / L, 40μmol / L, 60μmol / L, 80μmol / L, 100μmol / L respectively, and mark the above dilutions and 100mmol / L PIPES aqueous solution as H 2 O 2 Working solution; then take 100μL of the above H 2 O 2 The working solution was mixed with 100μL of TMB (tetramethylbenzidine) aqueous solution with a concentration of 20mmol / L, 2μL of horseradish peroxidase (purchased from TaKaRa) with a concentration of 1mg / mL, incubated at 16°C for 10min, and measured OD 630 v...

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Abstract

The invention discloses lactobacillus crispatus and application thereof. The perseveration number of the lactobacillus crispatus is as follows: CGMCC No.9388. The lactobacillus crispatus IMC-12 disclosed by the invention has relatively strong capacity of producing H2O2 and can be used for effectively inhibiting the growth of external pathogenic bacteria and other conditioned pathogenic bacteria and meanwhile, the lactobacillus cripatus has relatively strong adhesive attraction for vagina epithelial cells, so that the field planting of the vagina epithelial cell is guaranteed, and thus, the lactobacillus crispatus IMC-12 has certain application prospect in preventing and treating urogenital tract infection.

Description

Technical field [0001] The invention relates to a strain of Lactobacillus flexus and its application, and belongs to the field of microorganisms. Background technique [0002] Genitourinary tract infection is the most common but under-appreciated frequently-occurring disease in women. The incidence rate is second only to respiratory tract infection, which can be as high as 40%. Female genitourinary tract infection is the general term for urinary tract infection and reproductive tract infection. It not only directly affects Women's physical and mental health will also affect the happiness and stability of many families, and then affect the population quality and economic development of the whole society. The common pathogen of female urinary tract infections is gram-negative bacilli, among which Escherichia coli is the most common, followed by staphylococcus; most of these are the normal flora on the human intestine and skin mucosa. Due to the decline of the body’s immunity, these...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/741A61P13/00A61P15/00A61P31/04C12R1/225
Inventor 朱宝利张瑞芬李晶王江
Owner 北京益生苑生物科技有限公司
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