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A kind of method and fermentation medium formula of direct fermentation production L-sodium lactate

A fermentation medium and sodium lactate technology, applied in the field of fermentation medium of a method, can solve the problems of inexhaustible separation of methanol, high energy consumption and high cost, and achieve the advantages of reducing waste water discharge, low energy consumption and low production cost. Effect

Active Publication Date: 2017-11-10
HUBEI AIER BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are two kinds of existing sodium lactate production: one is directly synthesized with lactic acid, that is, food-grade lactic acid is directly reacted with ionic membrane alkali to generate sodium lactate, and the cost is higher; the other is the esterification method to produce sodium lactate. Crude lactic acid and methanol are esterified and rectified to obtain methyl lactate, and then the ionic membrane base is reacted with methyl lactate to generate sodium lactate and methanol, and methanol is distilled out; on the one hand, the energy consumption is high, and on the other hand, there is methanol separation endless potential danger

Method used

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  • A kind of method and fermentation medium formula of direct fermentation production L-sodium lactate
  • A kind of method and fermentation medium formula of direct fermentation production L-sodium lactate
  • A kind of method and fermentation medium formula of direct fermentation production L-sodium lactate

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Embodiment 1

[0038] A method for producing L-sodium lactate by direct fermentation, the steps are as follows:

[0039] 1. Fermentation medium:

[0040] Select glucose fermentation medium:

[0041] The formula of the fermentation broth is: glucose 230g / L, yeast powder 0.06g / L, sodium dihydrogen phosphate 0.04g / L, disodium hydrogen phosphate 0.04g / L, 1.5×10 -3 g / L riboflavin, 1.0×10 -3 g / L methionine, ammonium sulfate 1.5g / L.

[0042] Glucose is food-grade crystalline sugar produced by Xiwang Group;

[0043] Sodium hydroxide is ionic membrane caustic soda produced by Tianyuan Chemical Industry (reaching food grade);

[0044] Yeast powder is the Angel yeast extract powder of Angel Yeast Co., Ltd.;

[0045] Ammonium sulfate was purchased from Nanjing DSM Oriental Chemical Co., Ltd.;

[0046] Methionine and riboflavin were of reagent grade.

[0047] 2. Fermentation

[0048] When the temperature of the glucose ingredients drops to 55°C, inoculate lactic acid bacteria with 10% inoculum fo...

Embodiment 2

[0078] Direct production of sodium lactate using different fermentation media:

[0079] According to the method described in Example 1, the final product situation was detected with corn flour as the fermentation raw material, starch as the fermentation raw material and glucose culture medium of the present invention as the fermentation raw material.

[0080] Medium formula of corn flour: 200 mesh corn flour 230g / L

[0081] The starch medium formula is: starch 230g / L, 1% (mass volume ratio) corn steep liquor.

[0082] The lactic acid bacteria is Lactobacillus germanitis SZ01.

[0083] Other conditions or methods are the same as in Example 1.

[0084] Detect the output and product quality of L-sodium lactate after the fermentation is completed.

[0085] After the fermentation was completed, the output of L-sodium lactate in the fermentation broth obtained by fermentation of corn flour was 130.54g / L, and the fermentation period was 40h; the output of L-sodium lactate in the f...

Embodiment 3

[0094] Using conventional lactic acid bacteria to directly ferment and produce sodium lactate with glucose fermentation medium:

[0095] According to the method described in Example 1, the final product was detected using the glucose culture medium of the present invention as the fermentation raw material.

[0096] The lactic acid bacteria are strains from Chongqing Sanheng Biological Company. Fermentation is carried out under the fermentation condition of 45° C. to produce L-sodium lactate. After the fermentation, the output of L-sodium lactate in the fermentation broth was 118.84g / L, and the fermentation period was 40h.

[0097] Other conditions or methods are the same as in Example 1.

[0098] Detect the output and product quality of L-sodium lactate after the fermentation is completed.

[0099] Its quality standard of the sodium lactate solution of the 60% concentration that makes after the step decolouring of embodiment 1, filters, concentrates is as follows:

[0100]...

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Abstract

The invention discloses a method for producing sodium L-lactate by direct fermentation and a fermentation culture medium formula. The method comprises the following steps: cooling the sterile fermentation culture medium to 50-60 DEG C, and inoculating lactobacillus according to the inoculum size of 5-10% to perform fermentation, wherein the tank pressure is kept at 0.02-0.03MP, the pH value is kept at 5.0-5.5 by using 20% food-grade sodium hydroxide, the fermentation temperature is 50-55 DEG C, the fermentation is finished when the residual sugar is 0, and the sodium lactate yield after the fermentation finishes is 150 g / l; and removing saccharines and bivalent above metals through a 8040 film, concentrating, and inspecting the obtain the sodium L-lactate which completely satisfies the national standard GB-25537-2010. The method disclosed by the invention reduces the wastewater discharge, does not have the potential food safety problem, has the advantages of lower energy consumption, fewer production steps and the production cost, and has obvious application prospects.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for directly fermenting and producing L-sodium lactate, and also relates to a fermentation medium suitable for the method for directly fermenting and producing L-sodium lactate. Background technique [0002] L-sodium lactate can be used as an emulsifier, humectant, flavor improver, quality improver, antioxidant synergist and pH regulator. It is widely used in the meat and poultry food processing industry, which can enhance the flavor, inhibit the growth of pathogenic bacteria in food, Extend product shelf life. [0003] As a food preservative, flavoring agent, antifreeze, humectant, etc., sodium lactate has been used in the food industry instead of sodium benzoate as a preservative in some countries. It is considered a "safe and non-toxic substance" by the US Food and Drug Administration. [0004] There are two kinds of existing sodium lactate production: one is directly sy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/56C12R1/225
CPCC12P7/56
Inventor 刘锋明晓东雷滟敏
Owner HUBEI AIER BIOTECH CO LTD