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Pear peel extract as well as extraction method and application thereof in blood glucose-reduction health products
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An extraction method and extract technology, applied in the preparation of hypoglycemic health food, pear peel extract and extraction field, can solve the problems such as glycosidase inhibitory effect has not been reported
Inactive Publication Date: 2015-01-21
TIANJIN UNIV
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However, the inhibitory effect of pear peel extract on glycosidase has not been reported.
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Embodiment 1
[0030] The extraction method of pear peel extract, comprises the steps:
[0031] (1) Freeze-dry the pear skin of the Yaguang pear in a freeze dryer at -50°C for 15 hours;
[0032] (2) Mix the dried pear skin with methanolaqueous solution with a volume concentration of 60% according to the mass ratio of 1:50, extract by ultrasonic (power 50HZ, 220V) for 1 hour, extract 3 times, combine the extracts, concentrate, 50 Dry at ℃ to completely evaporate the methanol to obtain the extract.
[0033] The extraction method of the pear flesh extract is the same as the extraction method of the pear skin extract in this embodiment.
[0034] Substitute Qiuzi pear or Nanguo pear for Yaguang pear in this example, and other steps are the same as in this example to obtain the corresponding pear skin extract.
Embodiment 2
[0036] The extraction method of pear peel extract, comprises the steps:
[0037] (1) the pear skin of Snowflake pear is vacuum-dried at 45° C. in a vacuum drying oven;
[0038] (2) Mix the dried pear skin with methanol aqueous solution with a volume concentration of 50% according to the mass ratio of 1:50, ultrasonically extract for 0.5 hours, extract 3 times, combine the extracts, concentrate, and dry at 60°C to completely volatilize the methanol Dried extract.
[0039] Korla fragrant pear or duck pear is substituted for snowflake pear in this example, and other steps are the same as in this example to obtain the corresponding pear skin extract.
Embodiment 3
[0041] The extraction method of pear peel extract, comprises the steps:
[0042] (1) Freeze-drying the pear skin of Huanghua pear in a freeze dryer at -40°C for 15 hours;
[0043] (2) Mix the dried pear skin with 80% ethanol aqueous solution at a mass ratio of 1:100, ultrasonically extract for 1.5 hours, extract twice, combine the extracts, concentrate, and dry at 50°C to completely volatilize the ethanol Dried extract.
[0044] Replace the Huanghua pear in this example with the new pear, and the other steps are the same as in this example to obtain the corresponding pear skin extract.
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Abstract
The invention discloses a pear peel extract as well as an extraction method and an application thereof in blood glucose-reduction health products. The extraction method comprises the following steps: carrying out freezing and drying or vacuum drying on pear peels, mixing the dried pear peel and a methyl alcoholaqueous solution or ethyl alcoholaqueous solution with the concentration of 50-80% in a mass ratio of 1:(10-100), ultrasonically extracting for 0.5-2h for 2-3 times, combining extraction solutions, concentrating, and drying at 40-60 DEG C to enable methyl alcohol or ethyl alcohol to be completely volatilized to obtain extract. The pear peel extract has an obvious effect on reducing blood glucose of diabetic mice, and also has obvious effects on glucose tolerance adjustment, postprandial blood glucose delaying, and improving oxidative stress (improving SOD (superoxidedismutase) activity and reducing MDA (Methane Dicarboxylic Aldehyde) content) of the diabetic mice through in-vivo tests; and the pear peel extract has a remarkable inhibition effect on alpha-glycosidase through in-vitro tests, therefore, the pear peel extract has the effect on postprandial blood glucose delaying.
Description
technical field [0001] The invention belongs to the field of health food, and in particular relates to an extract of pear peel, its extraction method and its use in preparing health food for lowering blood sugar. Background technique [0002] Pear is a deciduous tree of Rosaceae, Maloideae, and Pyrus. Pear is a kind of fruit rich in phenolic acid and flavonoids. Pear peel is usually regarded as waste, and its value is often neglected. Therefore, the systematic identification of chemical components in pear peel is beneficial to the resource utilization and development of pear peel. Pear peel is rich in flavonoids and organic phenolic acids, which have pharmacological activities such as antibacterial, anti-inflammatory, anti-tumor, and prevention of coronary heart disease and stroke, but there is no report on its hypoglycemic effect. [0003] Diabetes mellitus is an endocrine disease characterized by chronic hyperglycemia, glucose and lipid metabolism, and proteinmetabolism ...
Claims
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