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Method for killing tumor cells through combination of gold-mediated near-infrared light heat effect and autophagy inhibitor

A technology of autophagy inhibitor and photothermal effect, which is applied in the direction of antineoplastic drugs, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problem of small toxic and side effects, and reduce the risk of harm , good tumor cell killing effect

Active Publication Date: 2015-02-18
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has the characteristics of less toxic and side effects and good effect of targeted therapy, and its related preclinical and clinical research trials are in the ascendant

Method used

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  • Method for killing tumor cells through combination of gold-mediated near-infrared light heat effect and autophagy inhibitor
  • Method for killing tumor cells through combination of gold-mediated near-infrared light heat effect and autophagy inhibitor
  • Method for killing tumor cells through combination of gold-mediated near-infrared light heat effect and autophagy inhibitor

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Preparation and Characterization of Gold Nanocages

[0033] Synthetic preparation of silver nanocubes:

[0034]Add 100mL of ethylene glycol into a 250mL round bottom flask, then put it into an oil bath at 150°C for 1 hour and stir it magnetically. 1.2mL, 3mM sodium hydrosulfide (NaHS) ethylene glycol solution was added to the reaction solution; 2 minutes later, 10mL, 3mM hydrochloric acid (HCl) ethylene glycol solution was added to the reaction solution; followed by 25mL, 20mg / mL Polyvinylpyrrolidone (PVP, molecular weight ~55000); after 2 minutes, 8 mL of 282 mM silver trifluoroacetate (CF3COOAg). The reaction was carried out for about 20 minutes, and the absorption spectrum of the reaction solution was determined to be around 440nm (corresponding to silver nanocubes around 40nm) by an ultraviolet-visible absorption spectrometer, and the reaction flask was quickly placed in an ice-water bath to terminate the reaction. The obtained silver nanocubes were was...

Embodiment 2

[0038] Embodiment 2: the photothermal conversion curve of gold nanocage

[0039] Mix 400 μL of DMEM, PBS, 400 μL PBS containing 100 μg / mL polyvinyl pyrrolidone (Polyvinyl Pyrrolidone, PVP) and 400 μL system PBS containing 0.5, 1, 2, 2.5, 3, 4, 5 μg / mL gold nanocages respectively , placed in a transparent round-bottom glass tube, using MDL-III-808nm-2.5w laser (Changchun New Industry Optoelectronics Technology Co., Ltd.) to irradiate the bottom of the tube with an output power of 1w. At this time, the laser is not connected to the coupling fiber. And use the thermocouple to measure the temperature of the liquid in the tube in real time. Such as figure 2 As shown, the results show that gold nanoparticles have superior light-to-heat conversion ability.

Embodiment 3

[0040] Embodiment 3: cell culture and cell viability experiment

[0041] Human cervical cancer cell line HeLa cells and mouse breast cancer cell line 4T1 were purchased from ATCC. Microtubule-associated light chain 3 (LC3) plasmid was a gift from N. Mizushima (Tokyo, Japan). Construction of HeLa cells stably transfected with GFP-LC3 based on this GFP-LC3 plasmid 【Zhang, Q.; Yang, W.J.; Man, N.; Zheng, F.; Shen, Y.Y.; Sun, K.J.; Li, Y.; Wen ,L.P.,Autophagy-mediated chemosensitization in cancer cells by fullerene C60nanocrystal.Autophagy 2009,5(8),1107-1117.] Human cervical cancer cell line HeLa cells, mouse breast cancer cells 4T1 and HeLa cells stably expressing GFP-LC3 (GFP-LC3 / HeLa) cultured at 37°C, CO 2 In an incubator with a concentration of 5%, DMEM medium (containing 10% fetal bovine serum) contains 100 units / mL of penicillin / streptomycin and 2.5 μg / mL of amphotericin B. GFP-LC3 / HeLa cells were maintained with G418 for a period of time before use. Follow the princip...

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Abstract

The invention provides a method for killing tumor cells through combination of a gold-mediated near-infrared light heat effect and an autophagy inhibitor. Compared with the prior art, the use quantity of gold nanoparticles can be reduced due to introduction of the autophagy inhibitor, and a god tumor cell killing effect can be obtained by a low dose of laser, so that the injury risk of laser and gold nanoparticles on normal cell tissue is reduced.

Description

technical field [0001] The invention relates to the combination of gold-mediated near-infrared photothermal effect and autophagy inhibitors for killing tumors. Background technique [0002] Cancer is an incurable disease that occurs in various groups of people, ages and different human organs due to the mutation of related genes that control the cell cycle and the accumulation of genetic instability, which seriously threatens human health and life. At present, there is no effective diagnosis and treatment method that can completely cure cancer. [0003] At present, the main means of treating cancer are: surgical operation to directly remove the tumor at the lesion, radiotherapy, chemotherapy or a combination of the above treatment means. However, surgical treatment is difficult to remove all tumor tissues, and surgical treatment will become helpless for parts where tumor resection is difficult or non-solid tumors. Radiotherapy and chemotherapy are limited by the inability ...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K47/04A61K47/02A61K47/34A61P35/00
Inventor 温龙平魏鹏飞张强周伟张云娇梁朝朝张力石闪闪
Owner UNIV OF SCI & TECH OF CHINA
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