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ph fluorescent sensing film and method for detecting ph two-dimensional dynamic distribution of basic sediment

A technology for fluorescent sensing films and deposits, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problem of lack of fluorescent sensing films, and achieve the effect of broadening the scope of application, improving accuracy, and avoiding optical interference.

Inactive Publication Date: 2015-02-18
NANJING INST OF GEOGRAPHY & LIMNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The technical problems of pH monitoring in alkaline sediments are reflected in two aspects: 1) the lack of good alkaline pH fluorescent sensing membranes, which are sensitive to pH changes; 2) how to reduce the interference of factors such as sediment background, light source attenuation, and film bleaching , so the flat electrode technology for pH measurement of alkaline sediments has not been reported yet

Method used

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  • ph fluorescent sensing film and method for detecting ph two-dimensional dynamic distribution of basic sediment
  • ph fluorescent sensing film and method for detecting ph two-dimensional dynamic distribution of basic sediment
  • ph fluorescent sensing film and method for detecting ph two-dimensional dynamic distribution of basic sediment

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Effect test

Embodiment 1

[0038] Embodiment 1 pH fluorescent sensing film and its preparation

[0039] (1) Synthesis of fluorescent dye CPIPA

[0040] p-Chloroaniline, p-diaminocinnamaldehyde is dissolved in methanol / dichloromethane (5:2v / v) mixed solvent according to the molar mass ratio of 1:1, fully reacted at room temperature, obtains CPIPA after filtration and recrystallization, passes through thin layer Compound identification was obtained by silica gel plate running or mass spectrometry ( figure 1 ), the results show that the CPIPA purity is more than 99.99% in the obtained material.

[0041] For the spectral characteristics of the fluorescent dye CPIPA, see figure 2 .

[0042] (2) Take 120mg polyvinyl chloride pellets, add 240μl plasticizer, shake and shake well, then add 1mL tetrahydrofuran, add two kinds of fluorescent dyes CPIPA and Fluorescent Yellow 10-GN respectively (according to the concentration of the obtained mixture 1% and 2% , wt / wt), and 3.0 mg tetrakis (4-chlorophenyl) potas...

Embodiment 2

[0044] Example 2 standard curve drawing

[0045] (1) Use 0.1M sodium phosphate and sodium dihydrogen phosphate to make a mixed buffer solution, then use sodium hydroxide and dilute hydrochloric acid to adjust the pH value, so that the pH value of the solution changes between 6-11 with a range of about 0.5 units , when adjusted to a stable pH value, the 389nm UV-LED light source was used to excite, and the fluorescence ratio image acquisition system ( image 3 ) to obtain fluorescence images under different pH conditions.

[0046] (2) For the fluorescent image obtained in (1), use the image processing software ImageJ 1.46 to split the RGB three-channel, split into images of different fluorescence intensities, and obtain the R and B-channel fluorescence intensity image, calculate the fluorescence intensity ratio I(R / B) of the two channels, and perform curve fitting with the corresponding pH value to obtain the response curve of the membrane to pH, and the fluorescence release c...

Embodiment 3

[0052] Example 3 pH Fluorescent Sensing Membrane Uniformity and Response Time Test

[0053] (1) Uniformity test

[0054] Stick the sensing film in the solution with a pH value of 7, and take pictures after it is stabilized. The obtained pictures were first calculated with the ImageJ software to calculate the ratio of the R channel and the B channel, and then used the Matlab software for drawing. The RSD that obtains sensing film in the scope of 5cm * 5cm is 1.55% ( Figure 4 ).

[0055] (2) Response time test

[0056] Stick the sensing membrane in a solution with a pH value of 7, and take a picture every 10 seconds. When it reaches 130 seconds, immediately take out the sensing membrane and put it in a solution with a pH value of 9, and take pictures every 10 seconds a picture. After the picture is analyzed with ImageJ software, the ratio of the R channel and the B channel of the picture is obtained, and the ratio is taken as the ordinate, and the shooting time is taken as...

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Abstract

The invention discloses a pH fluorescent sensing film which comprises a film base material and is characterized in that fluorescent dyes of CPIPA (Chloro Phenyl Imino Propenyl Aniline) and fluorandiol 10-GN are uniformly mixed together, and the two fluorescent dyes are fixed on the surface of the film base material by virtue of a chemical embedding method. On the basis of the pH fluorescent sensing film, the invention also discloses a method for detecting pH two-dimensional dynamic distribution of basic sediment, wherein the pH fluorescent sensing film is put into the sediment, spectral information of the two dyes is obtained by virtue of an imaging device under the illumination of exciting light, and distributional characteristics of the pH of the sediment are obtained by virtue of R and B passage fluorescent ratio quantification. According to the sensing film disclosed by the invention, the CPIPA and the fluorandiol 10-GN which has a reference effect and a brightening effect are mixed together to form a fluorescent indicator, and the sensing film has high detection sensitivity and high spatial resolution and is sensitive to respond pH changes. The method adopts a plate electrode technology to realize real-time and in-situ acquisition of pH two-dimensional and dynamic distribution information of the basic sediment by reducing optical interference by virtue of fluorescent ratio quantification.

Description

technical field [0001] The invention relates to a flat electrode pH measurement technology, in particular to a pH fluorescent sensing film and a method for detecting the two-dimensional dynamic distribution of the pH of alkaline sediments. Through the fluorescence analysis method, two-dimensional detection of the pH of lake or ocean alkaline sediments is realized. Dimensional and dynamic distribution information can be obtained online in real time, and it is also suitable for the detection of pH in the alkaline range of water and soil. Background technique [0002] Sediment, as an important reservoir of nutrients in lakes, is an important source of nutrients in water bodies. The migration and transformation process of pollutants or nutrients in sediments is one of the most active research directions in the field of environmental microcosmic research. The study of the environmental behavior of nutrients and pollutants at the sediment-water interface has always been valued by ...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 韩超姚磊丁士明
Owner NANJING INST OF GEOGRAPHY & LIMNOLOGY
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