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Preparation method for TPGS medicine carrying lipidosome-natural material composite nano-fiber bracket

A technology of composite nanofibers and natural materials, which is applied in the field of drug-loaded liposomes composited with natural material nanofiber scaffolds, can solve the problems of limiting clinical application and industrial production, low drug loading rate, thermodynamic instability, etc., and achieve drug loading And the effects of high transfection efficiency, good biocompatibility, and good application prospects

Inactive Publication Date: 2015-03-04
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, liposomes composed of phospholipids and cholesterol are thermodynamically unstable systems with poor stability in vivo and in vitro, and their shortcomings such as low drug loading rate and short half-life severely limit their clinical application and industrial production.

Method used

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  • Preparation method for TPGS medicine carrying lipidosome-natural material composite nano-fiber bracket
  • Preparation method for TPGS medicine carrying lipidosome-natural material composite nano-fiber bracket
  • Preparation method for TPGS medicine carrying lipidosome-natural material composite nano-fiber bracket

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation of the TPGS liposome-silk fibroin composite nanofiber scaffold loaded with doxorubicin consists of the following three steps:

[0032] Step 1: film dispersion method to prepare drug-loaded liposomes modified by TPGS: take an appropriate amount of lecithin, cholesterol and doxorubicin (mass ratio 20:2:1) and add them to a round bottom flask, then add absolute ethanol, After stirring and dissolving at room temperature, place it on a rotary evaporator to completely remove ethanol and obtain a lipid film; prepare a 0.5% (mass volume ratio) TPGS aqueous solution, add it to the above lipid film, stir at room temperature for 2 hours, and use Ultrasonic cell pulverizer ultrasonically obtains the drug-loaded liposome suspension modified by TPGS with uniform particle size; the TEM picture of the prepared drug-loaded liposome modified by TPGPS is as follows: figure 1 shown. TPGS is wrapped around drug-loaded liposomes in cloudy form, and the multilayer structure o...

Embodiment 2

[0036] The preparation of the TPGS liposome-fish gelatin composite nanofiber scaffold loaded with epidermal growth factor consists of the following three steps:

[0037] Step 1: Preparation of drug-loaded liposomes modified by TPGS by film dispersion method: Weigh lecithin, cholesterol and epidermal growth factor (mass ratio 20:2:1) into a round bottom flask, then add absolute ethanol, room temperature After stirring and dissolving, place it on a rotary evaporator to completely remove ethanol and obtain a lipid film; prepare a 0.5% (mass volume ratio) TPGS aqueous solution, add it to the above lipid film, stir at room temperature for 2 hours, and use ultrasonic The cell pulverizer ultrasonically obtains the drug-loaded liposome suspension modified by TPGS with uniform particle size;

[0038] Step 2: Prepare an aqueous solution containing 20% ​​(mass volume ratio) fish gelatin, and add the drug-loaded liposome suspension modified by TPGS prepared in step 1 in a ratio of 10%-20%...

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Abstract

The invention provides a preparation method for a TPGS medicine carrying lipidosome-natural material composite nano-fiber bracket. The preparation method comprises the following three steps: step 1: adopting a thin film dispersing method to prepare the medicine carrying lipidosome decorated by polyethyleneglycol Vitamin E succinic acid ester TPGS; step 2: preparing aqueous solution containing a natural material; adding the aqueous solution in the medicine carrying lipidosome suspension decorated by the TPGS; sealing and stirring until the solution is transparent to obtain mixed spinning solution; step 3: conducting static spinning by adopting the mixed spinning solution prepared in the step 2 to obtain the natural material composite nano-fiber bracket loading the medicine carrying lipidosome decorated by TPGS. The composite nano-fiber bracket has favorable biocompatibility, and is high in medicine loading capacity and transfection efficiency; the preparation method is simple, and mild in reaction conditions; the composite nano-fiber bracket has unique advantages when the bracket is used as a medicine slow release carrier and a tissue engineering scaffold, and has broad application prospect in the field of biomedicine.

Description

technical field [0001] The invention belongs to the field of preparation of composite nanofiber membranes, in particular to a method for preparing a drug-loaded liposome composite natural material nanofiber scaffold modified by polyethylene glycol vitamin E succinate (TPGS) based on electrospinning technology method. Background technique [0002] Tissue engineering has high requirements on scaffold materials, which require scaffold materials to have good biocompatibility, low immunity, and biodegradability. At present, there are two main categories of the most widely used, one is artificial synthetic materials, and the other is natural biological materials. Compared with artificial synthetic materials, the advantage of natural materials is that they are directly taken from the organism and have good biocompatibility and biodegradability; in addition, natural materials themselves are similar to the structure of extracellular matrix, which can promote cell adhesion, prolifer...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/54
Inventor 王红声林思陈梦霞范林鹏余凡杨兴兴孙彬彬
Owner DONGHUA UNIV
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