Preparation method for TPGS medicine carrying lipidosome-natural material composite nano-fiber bracket

A technology of composite nanofibers and natural materials, which is applied in the field of drug-loaded liposomes composited with natural material nanofiber scaffolds, can solve the problems of limiting clinical application and industrial production, low drug loading rate, thermodynamic instability, etc., and achieve drug loading And the effects of high transfection efficiency, good biocompatibility, and good application prospects

Inactive Publication Date: 2015-03-04

DONGHUA UNIV

3 Cites 11 Cited by

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Problems solved by technology

However, liposomes composed of phospholipids and cholesterol are thermodynamically unstable systems with poor stability in vivo and in vitro,...

Abstract

The invention provides a preparation method for a TPGS medicine carrying lipidosome-natural material composite nano-fiber bracket. The preparation method comprises the following three steps: step 1: adopting a thin film dispersing method to prepare the medicine carrying lipidosome decorated by polyethyleneglycol Vitamin E succinic acid ester TPGS; step 2: preparing aqueous solution containing a natural material; adding the aqueous solution in the medicine carrying lipidosome suspension decorated by the TPGS; sealing and stirring until the solution is transparent to obtain mixed spinning solution; step 3: conducting static spinning by adopting the mixed spinning solution prepared in the step 2 to obtain the natural material composite nano-fiber bracket loading the medicine carrying lipidosome decorated by TPGS. The composite nano-fiber bracket has favorable biocompatibility, and is high in medicine loading capacity and transfection efficiency; the preparation method is simple, and mild in reaction conditions; the composite nano-fiber bracket has unique advantages when the bracket is used as a medicine slow release carrier and a tissue engineering scaffold, and has broad application prospect in the field of biomedicine.

Application Domain

Prosthesis

Technology Topic

ElectrospinningChemistry +9

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Experimental program(2)

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[0030] Example 1

[0031] The preparation of doxorubicin-loaded TPGS liposome-silk fibroin composite nanofiber scaffold consists of the following 3 steps:

[0032] Step 1: Preparation of drug-loaded liposomes modified by TPGS by thin film dispersion method: Weigh an appropriate amount of lecithin, cholesterol and doxorubicin (mass ratio 20: 2: 1) into a round bottom flask, and then add absolute ethanol. After stirring and dissolving at room temperature, place it on a rotary evaporator and evaporate completely to remove the ethanol to obtain a lipid film; prepare a 0.5% (mass volume ratio) TPGS aqueous solution, add it to the lipid film, and stir at room temperature for 2 hours. Ultrasonic cell pulverizer ultrasound to obtain uniform particle size suspension of drug-loaded liposome modified by TPGS; the TEM picture of the prepared drug-loaded liposome modified by TPGPS is as follows figure 1 Shown. TPGS is wrapped around the drug-loaded liposome in a cloud shape, and the multi-layer structure of the wrapped drug-loaded liposome is still intact. The liposome modified by TPGS has good stability and is beneficial to protect the activity of the loaded drug.

[0033] Step 2: Prepare an aqueous solution containing 20% (mass to volume) silk fibroin, and add the TPGS-modified drug-loaded liposome suspension prepared in step 1 in a ratio of 10%-20% by volume to it, and seal Stir until transparent;

[0034] Step 3: Let the mixed solution prepared in step 2 stand for a few minutes and then transfer it to the syringe, adjust the spinning voltage to 20kV, the injection pump advance rate to 0.5ml/h, and use aluminum foil to receive, the receiving distance is 15cm, and the load is prepared Adriamycin-based TPGS liposome-silk fibroin composite nanofiber scaffold ( figure 2 with image 3 TEM images and SEM images of the obtained composite nanofiber scaffold respectively), the average diameter of the obtained nanofibers is 467±30nm. The nanofibers prepared by this method have good morphology and uniform thickness ( image 3 ), and drug-loaded liposomes modified by TPGS can be successfully loaded on nanofibers ( figure 2 As shown by the middle arrow), this new type of scaffold facilitates the stable and long-lasting release of doxorubicin and can better exert its anti-tumor effect.

Example Embodiment

[0035] Example 2

[0036] The preparation of TPGS liposome-fish gelatin composite nanofiber scaffold loaded with epidermal growth factor consists of the following 3 steps:

[0037] Step 1: Preparation of drug-loaded liposomes modified by TPGS by thin film dispersion method: Weigh lecithin, cholesterol and epidermal growth factor (mass ratio 20: 2: 1) into a round bottom flask, then add absolute ethanol at room temperature After dissolving under stirring, place it on a rotary evaporator and evaporate completely to remove the ethanol to obtain a lipid film; prepare 0.5% (mass to volume ratio) TPGS aqueous solution, add it to the lipid film, stir at room temperature for 2 hours, and then use ultrasound The cell pulverizer ultrasound to obtain a drug-loaded liposome suspension modified with TPGS with uniform particle size;

[0038] Step 2: Prepare an aqueous solution containing 20% (mass to volume) fish gelatin, and add the TPGS-modified drug-loaded liposome suspension prepared in step 1 in a volume ratio of 10%-20%, and seal and stir To transparent

[0039] Step 3: Transfer the mixed solution prepared in step 2 to a syringe, adjust the spinning voltage to 20kV, the injection pump advance rate to 0.5ml/h, the receiving distance to 15cm, and the aluminum foil to receive it to prepare TPGS loaded with epidermal growth factor The liposome-fish gelatin composite nanofiber scaffold has good nanofiber morphology and uniform thickness (average diameter is 472±30nm). The addition of TPGS drug-loaded liposome has no significant effect on the fiber morphology ( Figure 4 ). The scaffold system can release epidermal growth factor stably and persistently, which is beneficial to induce the regeneration of related tissues.

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Classification and recommendation of technical efficacy words

easy to operate
good biocompatibility

Preparation method for TPGS medicine carrying lipidosome-natural material composite nano-fiber bracket

AI-Extracted Technical Summary

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Abstract

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Description & Claims & Application Information

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