Preparation method for TPGS medicine carrying lipidosome-natural material composite nano-fiber bracket
A technology of composite nanofibers and natural materials, which is applied in the field of drug-loaded liposomes composited with natural material nanofiber scaffolds, can solve the problems of limiting clinical application and industrial production, low drug loading rate, thermodynamic instability, etc., and achieve drug loading And the effects of high transfection efficiency, good biocompatibility, and good application prospects
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[0030] Example 1
[0031] The preparation of doxorubicin-loaded TPGS liposome-silk fibroin composite nanofiber scaffold consists of the following 3 steps:
[0032] Step 1: Preparation of drug-loaded liposomes modified by TPGS by thin film dispersion method: Weigh an appropriate amount of lecithin, cholesterol and doxorubicin (mass ratio 20: 2: 1) into a round bottom flask, and then add absolute ethanol. After stirring and dissolving at room temperature, place it on a rotary evaporator and evaporate completely to remove the ethanol to obtain a lipid film; prepare a 0.5% (mass volume ratio) TPGS aqueous solution, add it to the lipid film, and stir at room temperature for 2 hours. Ultrasonic cell pulverizer ultrasound to obtain uniform particle size suspension of drug-loaded liposome modified by TPGS; the TEM picture of the prepared drug-loaded liposome modified by TPGPS is as follows figure 1 Shown. TPGS is wrapped around the drug-loaded liposome in a cloud shape, and the multi-laye...
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[0035] Example 2
[0036] The preparation of TPGS liposome-fish gelatin composite nanofiber scaffold loaded with epidermal growth factor consists of the following 3 steps:
[0037] Step 1: Preparation of drug-loaded liposomes modified by TPGS by thin film dispersion method: Weigh lecithin, cholesterol and epidermal growth factor (mass ratio 20: 2: 1) into a round bottom flask, then add absolute ethanol at room temperature After dissolving under stirring, place it on a rotary evaporator and evaporate completely to remove the ethanol to obtain a lipid film; prepare 0.5% (mass to volume ratio) TPGS aqueous solution, add it to the lipid film, stir at room temperature for 2 hours, and then use ultrasound The cell pulverizer ultrasound to obtain a drug-loaded liposome suspension modified with TPGS with uniform particle size;
[0038] Step 2: Prepare an aqueous solution containing 20% (mass to volume) fish gelatin, and add the TPGS-modified drug-loaded liposome suspension prepared in ste...
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