Glucose oxidase-mediated free radical initiating system and method for preparing hydrogel

A technology of glucose oxidase and triggering system, applied in the field of polymer compounds, can solve the problems affecting the subsequent application of hydrogel, incomplete polymerization of monomers, poor storage stability, etc., achieve excellent mechanical properties and biocompatibility, and formulate Simple and effective in a wide pH range

Inactive Publication Date: 2016-07-13
TONGJI UNIV
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  • Claims
  • Application Information

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Problems solved by technology

At the same time, after the ferrous ions in the system are oxidized to ferric ions, it will also inhibit the chain growth process of monomer free radicals, resulting in incomplete polymerization of monomers, which will affect the subsequent application of the prepared hydrogel.
Moreover, due to the strong reducibility of ferrous ions, it is very easy to oxidize in the air, so the storage stability of ferrous sulfate as one of the initiating components is poor after being made into an aqueous solution, which will also affect the actual use.
The above-mentioned enzyme-mediated polymerization initiation system can prepare hydrogels in a short time (about 3 minutes), but there is no report on the controllability of the initiation gelation time.

Method used

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  • Glucose oxidase-mediated free radical initiating system and method for preparing hydrogel

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Experimental program
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Effect test

Embodiment 1

[0029] 1) Preparation of precursor solution: take 0.10-0.13 g of N,N-dimethylacrylamide, 0.07-0.09 g of cross-linking agent polyethylene glycol diacrylate (average molecular weight 250), and 1.3-1.5 g of deionized water into the sample In the bottle, mix evenly with a vortex mixer, and the measured pH value is 7-8.

[0030] 2) Preparation of hydrogel: 100 μL of N-hydroxysuccinimide aqueous solution, 100 μL of glucose oxidase concentrated solution, and 200 μL of glucose aqueous solution were sequentially added to the above precursor solution (N-hydroxysuccinimide and glucose in the reaction system). The molar concentration ratio of glucose and glucose oxidase is 1.74, and the molar concentration ratio of glucose and glucose oxidase is 25000).

Embodiment 2

[0032] 1) Preparation of precursor solution: take N,N-dimethylacrylamide 0.10~0.13g, crosslinking agent polyethylene glycol diacrylate (average molecular weight 250) 0.07~0.09g, deionized water 1.3~1.5g, 1M Add 50 μL of acetic acid aqueous solution into the sample bottle, mix well with a vortex mixer, and the measured pH value is 3.5 to 4.5.

[0033] 2) Preparation of hydrogel: the steps are the same as in Example 1, and the gel formation time is 3min.

Embodiment 3

[0035] 1) Preparation of precursor solution: take N,N-dimethylacrylamide 0.10~0.13g, crosslinking agent polyethylene glycol diacrylate (average molecular weight 250) 0.07~0.09g, deionized water 1.3~1.5g, 1M 125 μL of aqueous ammonia solution was added to the sample bottle, and mixed evenly with a vortex mixer. The measured pH value was 9.5 to 10.5.

[0036] 2) Preparation of hydrogel: the steps are the same as in Example 1, and the gel formation time is 19min30s.

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Abstract

The invention relates to a glucose oxidase mediation free radical initiating system and a method for preparing hydrogel by using the glucose oxidase mediation free radical initiating system. The glucose oxidase mediation free radical initiating system consists of a N-hydroxyphthalimide derivative, glucose oxidase and glucose and has the advantages that the initiation condition is gentle, the preparation is simple and convenient, the pH value application range of a reaction medium is relatively wide, and the environment can be protected. According to the method provided by the invention, by adjusting the pH value of the reaction medium or adjusting the concentration ratio of components of a ternary initiating system, monomer polymerization can be initiated within 3-30 minutes at the room temperature under control to prepare hydrogel, and high-strength nano composite hydrogel can be also prepared, so that the method has remarkable application prospect in fields such as controlled-release of medicines, enzyme immobilization, tissue engineering and substance separation.

Description

technical field [0001] The invention relates to the field of polymer compounds, in particular to a novel glucose oxidase-mediated radical polymerization initiation system and a method for preparing hydrogels. Background technique [0002] Polymer compounds play an important role in the field of materials and are widely used in industry, agriculture, biomedicine, environmental protection, and civil life. Common polymer synthesis reactions usually require control of temperature and pressure, higher requirements for reaction equipment, and sometimes the use of toxic catalysts. With the increasing awareness of sustainable development of human society, it is the research direction of polymer synthesis chemistry to use efficient, non-toxic and environmentally friendly synthetic methods to prepare the required polymer products. [0003] Biological enzymes are proteins produced by living cells with catalytic functions and adjustable activities, and have the following characteristic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F220/54C08F222/14C08F120/54C08F290/02C08F226/10C08F4/40C08K3/34
Inventor 苏腾王启刚唐舟李汶军何鸿舰
Owner TONGJI UNIV
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