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Preparation method for unmarked electrogenerated chemiluminescence clenbuterol immune sensor and application thereof

An immunosensor, electrochemistry technology, applied in chemiluminescence/bioluminescence, electrochemical variables of materials, analysis by chemical reaction of materials, etc. Achieve the effect of improving detection sensitivity and reproducibility, convenient operation, and highly selective detection

Active Publication Date: 2015-04-08
河南安必诺检测技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the use of HRP requires harsh reaction conditions, which is unfavorable for Luminol-H 2 o 2 Universal use of luminescent systems

Method used

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  • Preparation method for unmarked electrogenerated chemiluminescence clenbuterol immune sensor and application thereof
  • Preparation method for unmarked electrogenerated chemiluminescence clenbuterol immune sensor and application thereof
  • Preparation method for unmarked electrogenerated chemiluminescence clenbuterol immune sensor and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of label-free electrochemiluminescent clenbuterol immunosensor

[0031] (1) Use a glassy carbon electrode with a diameter of 4 mm as the working electrode, and drop-coat 5 uL TiO on the surface of the electrode 2 NPs, after drying at room temperature, drop-coated 5 μL of AuAg NRs-CHS solution and dried at room temperature;

[0032] (2) Wash the electrode obtained in step (1) with ultrapure water, dry it at room temperature to form a film, and drop-coat 5uL 0.01 mol / L H 2 PdCl 4 , and immediately irradiated with a high-pressure mercury lamp for 30 seconds to obtain TiO 2 - AuAg-Pd NDRs-CHS modified working electrode, dry at room temperature;

[0033] (3) Wash the electrode obtained in step (2) with ultra-pure water, dry it at room temperature, immerse the electrode in the EDC / NHS solution, and take it out after 1 hour;

[0034] (4) Wash the electrode obtained in step (3) with ultrapure water, drop-coat 5 μL of 10 μg / mL clenbuterol antibody so...

Embodiment 2

[0041] Example 2 Preparation of label-free electrochemiluminescent clenbuterol immunosensor

[0042] (1) Use a glassy carbon electrode with a diameter of 4 mm as the working electrode, and drop-coat 8 uL TiO on the surface of the electrode 2 NPs, after drying at room temperature, drop-coated 8 μL of AuAg NRs-CHS solution and dried at room temperature;

[0043] (2) Wash the electrode obtained in step (1) with ultrapure water, dry it at room temperature to form a film, and drop-coat 8uL 0.01 mol / L H 2 PdCl 4 , and immediately irradiated with a high-pressure mercury lamp for 60 seconds to obtain TiO 2 - AuAg-Pd NDRs-CHS modified working electrode, dry at room temperature;

[0044] (3) Same as embodiment 1;

[0045] (4) Wash the electrode obtained in step (3) with ultrapure water, drop-coat 8 μL of 10 μg / mL clenbuterol antibody solution on the surface of the electrode, and store it in a refrigerator at 4 °C to dry;

[0046] (5) Wash the electrode obtained in step (4) with ...

Embodiment 3

[0051] Example 3 Preparation of label-free electrochemiluminescent clenbuterol immunosensor

[0052] (1) Use a glassy carbon electrode with a diameter of 4 mm as the working electrode, and drop-coat 10 uL TiO on the surface of the electrode 2 NPs, after drying at room temperature, drop-coated 10 μL of AuAg NRs-CHS solution and dried at room temperature;

[0053] (2) Wash the electrode obtained in step (1) with ultrapure water, dry it at room temperature to form a film, and drop-coat 10uL 0.01 mol / L H 2 PdCl 4 , and immediately irradiated with a high-pressure mercury lamp for 90 seconds to obtain TiO 2 - AuAg-Pd NDRs-CHS modified working electrode, dry at room temperature;

[0054] (3) Same as embodiment 1;

[0055] (4) Wash the electrode obtained in step (3) with ultrapure water, drop-coat 10 μL of 10 μg / mL clenbuterol antibody solution on the surface of the electrode, and store it in a refrigerator at 4 °C to dry;

[0056] (5) Wash the electrode obtained in step (4) w...

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Abstract

The invention discloses a preparation method for unmarked electrogenerated chemiluminescence clenbuterol immune sensor and belongs to the novel nano functional material and biosensor field. The dendrite nano rod-shaped precious metal alloy nano material having core-shell structure is prepared on the titanium dioxide nano-particle substrate by the photoelectric chemical synthesis method, and the unmarked electrogenerated chemiluminescence immune sensor used for detecting the clenbuterol can be prepared and the unmarked electrogenerated chemiluminescence immune sensor is low in cost, good in specificity, fast in detection speed and simple in preparation.

Description

technical field [0001] The invention relates to a preparation method and application of a label-free electrochemiluminescence immunosensor for fast and sensitive detection of clenbuterol, and belongs to the technical field of novel nanometer functional materials and biosensors. Background technique [0002] Clenbuterol is a class of drugs called beta-stimulants, not a specific drug. These drugs have the function of promoting the growth of lean meat and inhibiting the growth of fat meat, so they are collectively called "clenbuterol". According to the "Clenbuterol" Special Rectification Program (Food Safety Office [2011] No. 14) stipulated by the Office of the Food Safety Committee of the State Council, there are 16 varieties of "Clenbuterol", including: Ractopamine; Clenbuterol ; Salbutamol Salbutamol; Salbutamol Sulfate Salbutamol Sulfate; Dopamine Hydrochloride; Cimaterol; Terbutaline Sulfate; Clorprenaline Hydrochloride; Mabuterol; Cimbuterol; Brombuterol; Arformoterol T...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/48G01N21/76G01N33/53
Inventor 张勇马洪敏魏琴李燕吴丹庞雪辉
Owner 河南安必诺检测技术有限公司
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