Bacillus amyloliquefaciens lh-1 and its application
A technology for dissolving starch spores and bacilli, applied in the application, bacteria, fungicides and other directions, can solve the problems of increased production cost, complicated extraction process, etc., and achieves the effects of cost saving, simple production process and high viable bacteria count.
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Embodiment 1
[0017] Bacillus amyloliquefaciens of the present invention ( Bacillus amylolique faciens subsp ) Isolation and Identification of LH-1
[0018] 1. Sample collection
[0019] Sampling 100g from the soil surface to 15cm from the experimental field of Shanxi Academy of Agricultural Sciences, dried at 60°C for 24 hours, and took 1g for separation.
[0020] 2. Isolation and resistance screening of strains
[0021] (1) Strain dilution: Weigh 1g and dissolve it in a triangular flask filled with 100ml sterilized distilled water to make a soil suspension, and then gradiently dilute to 10 -9 soil suspension.
[0022] (2) Separation and screening of strains: absorb 50ul of soil suspension and 50ul of Verticillium dahliae bacteria in liquid culture for 3 days, add them to the cooled PDA medium, spread evenly, place them in an incubator at 30°C for upside-down cultivation for 24 hours, and observe And select antagonistic colonies (colonies with large inhibition zone);
[0023] (3) Puri...
Embodiment 2
[0031] Example 2 Determination of Bacillus amyloliquefaciens Lh-1 inhibition spectrum
[0032] Cotton Verticillium dahliae ( Verticillium dahliae ), Chinese cabbage soft rot ( Erwinia carotovora ), Ralstonia solanacearum ( Ralstonia solanacearum ), peach tree rot fungus ( Valsa leucostoma ), apple rot fungus ( Valsa mali Miyabe et Yamada ), Phytophthora capsici ( Phytophthora capsici ), Botrytis cinerea ( Phytophthora infestans ) as an indicator bacterium to determine the antibacterial effect of Bacillus amyloliquefaciens Lh-1. The specific method is as follows: under aseptic conditions, culture 48h, dilute 10 5 Double 50ul Bacillus amyloliquefaciens Lh-1 bacteria solution was spread evenly on the PDA plate medium, and cultured upside down in a 31°C incubator for 48h. After the colonies were inactivated by fumigation with chloroform for 20 minutes, a layer of soft agar containing indicator bacteria was used to spread a thin layer on the surface of the colonies, a...
Embodiment 3
[0037] Bacillus amyloliquefaciens ( Bacillus amylolique faciens subsp ) Preparation of Lh-1 probiotics
[0038] (1) Streak inoculate the Lh-1 strain stored in the -80°C refrigerator on the PDA solid slant medium, and cultivate it at 31°C for 48 hours to activate it. The activated strains were inoculated into PDA liquid medium, cultured with shaking at 31°C for 24 hours, and the bacterial liquid was used as the seed liquid for later use; (2) The raw materials and weight percentages for expanded fermentation production were as follows: corn flour 2%, bean cake 0.5%, 0.5% fish meal, 1% brown sugar, 0.1% sodium chloride, and the rest is water. Dissolve the raw materials in water according to the ratio and add them to the fermenter, sterilize with high-pressure steam, and inoculate the seed liquid obtained in step (1) into the fermenter. The inoculum amount accounts for 2-5% of the total volume of raw materials, preferably 3%, the stirring speed is 180r / min, 31°C, pH6.8, the relat...
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