Application of microbial agents and passivation of heavy metals in soil

A microbial agent and microbial composition technology, applied in the field of microbial composition, can solve the problems of reduced heavy metal content, insignificant improvement effect, limited soil/land adaptability, etc.

Active Publication Date: 2017-05-10
宋彦耕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The inventor is committed to the research of microbial soil improvement, and has obtained soil improvement technologies such as Chinese Patent No. 200910169275, and used 25 kinds of live microorganisms to obtain the Good treatment effect, wherein it is found that the heavy metal content in the plants planted on the soil polluted by heavy metals has a certain reduction effect; later, the inventor further researched and developed saline-alkali land improvement technologies such as Chinese Patent No. 201210441532, which reduced some bacteria In terms of the technology of the species, it provided a kind of improved bacterial agent for the original coastal saline-alkali land again, but no improvement effect on heavy metals was found.
However, the inventors have found that the soil / land adaptability of these previously developed microbial compositions / bacteria agents is limited, and the improvement effect on some saline-alkali lands (especially secondary saline-alkali lands) and sandy land is not significant, which affects the promotion effect

Method used

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  • Application of microbial agents and passivation of heavy metals in soil
  • Application of microbial agents and passivation of heavy metals in soil
  • Application of microbial agents and passivation of heavy metals in soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Production of microbial inoculum

[0050] In this example, the following strains were used to prepare microbial agents: (1) Acetobacter striatus ( Acetobacter aceti ), (2) Beauveria bassiana ( Beauveria bassiana ), (3) Azospirillum brasiliensis ( Azospirllum brasilense ), (4) Nocardia cellulose (Nocardia cellulans ), (5) Bacillus jelly-like (Bacillus mucilaginosus) , (6) Metarhizium anisopliae ( Metarhizium anisopliae ), (7) Trichoderma yellow-green (Trichoderma aureoviride) , (8) Spirulina obtuse ( Arthrospiraplatensis ), (9) Cellulomonas bean ( Cellulomonasfabia ), (10) Bacillus stearothermophilus ( Bacillus sterothermophilus ), (11) Lactobacillus casei ( Lactobacillus casei ), (12) Methanobacillus formate ( Methanobacterium formicium ), (13) Methanobacter ruminant ( Methanobacterium ruminantium ), (14) Thiobacillus thiooxidans ( Thiobacillus thiooxidans ), (15) Thiobacillus ferrooxidans ( Thiobacillus ferrooxidans ), (16...

Embodiment 2

[0053] Example 2 Production of improver

[0054] Take the cow dung with a water content of 65% (weight ratio) (add water if the water content is insufficient) and the pulverized rice bran with a particle size of less than 5mm and mix them evenly at a mass ratio of 85:15 and pulverize, so that the mixed cow dung and rice bran There is no agglomeration of more than 40mm.

[0055] Then, 1 ton of the above-mentioned mixture of cow dung and rice bran was evenly mixed with 1 kg of the microbial inoculum produced in Example 1, and piled into a fermentation tank to form a compost. During the placing of the compost heap, along with the fermentation process, the compost heap can naturally heat up. The fermentation and heating process usually takes 2-3 days, during which the compost heap can be turned 1-2 times. Then, when the temperature of the compost rises to 65°C, turn on the mixer to turn the compost to dissipate the heat generated by the fermentation to ensure that the temperature...

Embodiment 3

[0057] Example 3 Improvement example of secondary saline-alkali land in Tianjin Binhai New Area

[0058] Our previous patented technology has achieved good results on the coastal saline-alkali land in Tianjin Binhai New Area, but the effect on the secondary saline-alkali land caused by the degradation of the non-coastal former cultivated land is not significant, and the survival rate of seedlings is not ideal. Therefore, the use of new research and development The improvement test was carried out on the adjacent unmodified similar secondary saline-alkali land (and the phenomenon of desertification has occurred).

[0059] The test started on April 5, 2012, and was applied by Tianjin Taida Landscape Construction Co., Ltd. and Tianjin Haibin Avenue Construction and Development Co., Ltd. (during the period, no foreign soil was used), that is, the modifier particles prepared in Example 2 were mixed into the surface 20cm soil. , the mixing amount is 5kg per square meter, and the rot...

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PUM

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Abstract

The invention provides a microbial composition comprising acetobacter aceti, beauveria bassiana, azospirllum brasilense, nocardia cellulans, bacillus mucilaginosus, metarhizium anisopliae, trichoderma aureoviride and the like. The microbial composition can be stably combined in a microbial agent, is used for improving soil / land and especially can be used for effectively improving soil polluted by heavy metals.

Description

technical field [0001] The present invention belongs to the technical field of microbial fermentation, in particular, the present invention relates to a microbial composition. Although the number of bacterial species is large, the inoculum produced by it is stable, and the combination of these more bacterial species can effectively improve secondary saline-alkali soil and Sandy soil and other soils can also effectively improve heavy metal-contaminated soils. Background technique [0002] With the rapid development of global economy, especially the rapid development of industrial and mining industries, a large number of pollutants containing heavy metals are discharged. 15 million tons, Ni is 1 million tons. These heavy metal pollutants enter the soil through various ways, causing serious soil pollution. Heavy metal pollution in soil can affect the decline of crop yield and quality, cause serious damage to ecosystems, and especially endanger human health through the food ch...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/18C12N1/14C09K17/00B09C1/10C12R1/02C12R1/365C12R1/07C12R1/885C12R1/245C12R1/10C12R1/12C12R1/065C12R1/41C12R1/69C12R1/865C12R1/825C12R1/845C12R1/645C12R1/01
CPCB09C1/105C09K17/00C09K2101/00C12N1/14C12N1/18C12N1/20
Inventor 宋彦耕
Owner 宋彦耕
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