Immunochromatography kit for fluorescently and quantitatively detecting MBL (mannose-binding lectin)

Abstract

The invention relates to an immunochromatography kit for fluorescently and quantitatively detecting MBL (mannose-binding lectin). The immunochromatography kit comprises a fluorescence test strip and sample diluent, wherein the fluorescence test strip comprises a bottom plate, a sample pad, a fluorescence binding pad, an enveloped membrane and absorbent paper are sequentially bonded on the upper surface of the bottom plate from one end to the other end, the inner end of the sample pad is lap-jointed with one end of the fluorescence binding pad, the other end of the fluorescence binding pad is lap-jointed with one end of the enveloped membrane, and the other end of the enveloped membrane is lap-jointed with the absorbent paper, wherein the enveloped membrane comprises a detection zone and a quality control zone, the detection zone is enveloped with anti-MBL monoclonal antibody, and the quality control zone is enveloped with goat anti-rabbit IgG; and the fluorescence binding pad contains fluorescently-labeled MBL monoclonal antibody and fluorescently-labeled goat anti-rabbit IgG. The immunochromatography kit for fluorescently and quantitatively detecting MBL has strong specificity and high sensitivity, the detection result acquisition time is short, the operating mode is convenient and simple, and the detection result is accurate and reliable.

Description

technical field [0001] The invention relates to an immunochromatographic reagent kit for fluorescent quantitative detection of MBL and a preparation method thereof. Background technique [0002] Mannan (poly) sugar-binding lectin, English name: mannan-binding lectin, abbreviated as MBL. Mannose-binding lectin is a serum C-type lectin produced by the liver. It is an acute-phase protein that can bind to mannose. It can regulate pathogens with mannose, activate the complement system, participate in innate immune responses, activate complement, Regulates inflammation, promotes opsonophagocytosis, and clears apoptotic cells. It is one of the important components of the immune system and plays multiple roles in immune defense. When the adaptive immune system is immature (infancy) or has been suppressed (e.g. after organ transplantation, or during cancer chemotherapy) often the absence of MBL may lead to increased susceptibility to infection. MBL deficiency is significantly asso...

AI Technical Summary

Problems solved by technology

Restriction fragment length polymorphism methods, amplification hindrance mutation systems, and heteroduplex analysis methods used in early laboratories were very complicated, laborious, relatively expensive, and poor in accuracy

The gene chip technology developed in the late 1990s has a high degree of automation, the analysis time is greatly shortened, and the accuracy of the analysis results is also greatly improved. However, the cost is relatively high, and it is limited to laboratory research, and it is difficult to promote it in a large scale in clinical practice.

The only MBL detection reagent used for clinical diagnosis in the world is the kit developed by BioPorto, Denmark. The principle is to use the monoclonal antibody against MBL CRD (mannan-binding lectin sugar recognition domain) as the capture antibody, and the biotin label is the same. The antibody used as the detection antibody, HRP-SA (horseradish peroxidase-labeled streptavidin) as the amplified chromogenic system, the detection time of this method is longer than 4 hours, the operation is cumbersome, and the sensitivity can be detected to 0.5 ng / ml

However, automatic imported instruments and kits need to be used for detection, which are quite expensive and cannot realize rapid detection of large quantities of samples, which limits the promotion and application

However, there is no self-developed MBL detection kit in China

Images