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Gold-core-composite nano-carrier as well as preparation method and application thereof

A technology of nanocarriers and gold nanorods, applied in nanotechnology, nanotechnology, nanomedicine, etc.

Inactive Publication Date: 2015-05-06
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are few literature and patent reports on the application of near-infrared laser-induced nanoparticles in the delivery of tumor-targeted drugs.

Method used

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  • Gold-core-composite nano-carrier as well as preparation method and application thereof
  • Gold-core-composite nano-carrier as well as preparation method and application thereof
  • Gold-core-composite nano-carrier as well as preparation method and application thereof

Examples

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preparation example Construction

[0055] The preferred gold core composite nanocarrier preparation method of the present invention comprises the following steps:

[0056] (1) The gold nanorod solution modified with hexadecyltrimethylammonium bromide is used as raw material, wherein the length of the gold nanorod is 5-100nm, and the aspect ratio is 1.5-10.0; The rod concentration is 0.1-2mmol / L; the concentration of cetyltrimethylammonium bromide is 0.1-10mmol / L;

[0057] (2) Add sodium hydroxide or ammonia solution to the solution in step (1), make the pH of the solution be 8.0-12.0, then add methanol or ethanol solution of ethyl silicate three times, each interval 10-60min, react in Carrying out at 20-80°C for 1-4 days, wherein the molar ratio of gold nanorods in the gold nanorod aqueous solution to the ethyl silicate is 1:1-50 in terms of gold element and silicon element respectively;

[0058] (3) After the above step (2) is completed, add 3-(methacryloyloxy)propyltrimethoxysilane, the mass of which is 0.02...

Embodiment 1

[0069] The preparation of embodiment 1 gold core composite nano-carrier

[0070] 1) Silica-coated gold nanorods (AuSiO 2 ) preparation

[0071] In a 20mL beaker, mix 7.5mL of an aqueous solution of cetyltrimethylammonium bromide (concentration: 0.1mol / L) with 250 μL of an aqueous solution of chloroauric acid (concentration: 0.01mol / L), add water to The total volume is 9.4mL; then add 0.6mL aqueous sodium borohydride solution (concentration: 0.01mol / L) cooled by ice water; stir vigorously for 3min, place in a 27°C incubator for 3-5h, and obtain a gold nanoparticle seed solution .

[0072]In a 150mL beaker, add 100mL of cetyltrimethylammonium bromide (concentration is 0.1mol / L), 5mL of chloroauric acid aqueous solution (concentration is 0.01mol / L), 0.8mL of silver nitrate aqueous solution (concentration is 0.01mol / L), 2mL sulfuric acid aqueous solution (concentration is 0.5mol / L), and the ascorbic acid aqueous solution (concentration is 0.1mol / L) of 800 μ L; Then add the abov...

Embodiment 2

[0079] This example is used to illustrate the reversible process of the particle size of the gold-core composite nanocarrier changing with temperature, and the phase transition temperature of the temperature-sensitive gold-core composite nanocarrier can be judged by the speed of particle size change.

[0080] The gold-core composite nanocarrier prepared in Example 1 was centrifuged and washed once, then 2 mL was taken in a cuvette, and its particle size at different temperatures was measured with a laser particle size analyzer. From 25°C to 35°C, gradually increase the temperature every 5°C, from 35°C to 43°C, gradually increase the temperature every 2°C, from 43°C to 50°C, gradually increase the temperature every 3 or 4°C, equilibrate for 5 minutes at each temperature point, and The particle size of the gold-core composite nanocarriers was measured. Then gradually lower the temperature from 50°C, equilibrate for 5 minutes at each temperature point, and measure the particle si...

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Abstract

The invention relates to a gold-core-composite nano-carrier as well as a preparation method and application thereof. According to the gold-core-composite nano-carrier, a silicon dioxide modified gold nanorod (Au@SiO2) is taken as an inner core and is coated with a layer of high polymer material sensitive to temperature and pH. The gold-core-composite nano-carrier can be used for coating various drug and probe molecules and transporting the drug and probe molecules to disease sites, and therefore, the targeted therapy is realized. After absorbing near-infrared laser, the gold-core-composite nano-carrier or a drug-loading nanoparticle can emit fluorescent light, and partial optical energy can be converted into heat to be applied to biological imaging, chemotherapy and thermal therapy; the gold-core-composite nano-carrier further has the pH-sensitive property and is capable of effectively releasing the drug molecules in a weakly acidic tumor microenvironment and cell lysosome; multiple tumor therapy functions of the gold-core-composite nano-carrier can be combined in use, so that the defects of a single tumor therapy method are overcome; the gold-core-composite nano-carrier is suitable for being applied to the complex tumor diagnosis and treatment.

Description

technical field [0001] The invention belongs to the technical field of drug carriers, in particular to a drug carrier and its preparation method and application, in particular to a drug carrier based on gold core composite nano, its preparation method and its application in tumor diagnosis and treatment. Background technique [0002] In the past few decades, although people's understanding of cancer has made great progress, cancer is still one of the major diseases affecting human health and life expectancy. According to the 2012 China Cancer Registration Annual Report, there are about 3.5 million new cancer cases and 2.5 million cancer deaths in my country every year. The high mortality rate of cancer is largely due to the poor efficacy and severe side effects of traditional cancer treatments. In cancer treatment, the combination of hyperthermia and chemotherapy has been shown to optimize the effect of cancer treatment. If hyperthermia itself cannot completely remove canc...

Claims

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Application Information

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IPC IPC(8): A61K47/32A61K47/02A61K47/04A61K41/00A61K49/00A61K9/14A61P35/00B82Y5/00
Inventor 陈春英王静仉振江
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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