Enzyme-process extraction technology for chitin

An enzymatic extraction and chitin technology is applied in the field of enzymatic extraction of chitin, which can solve the problems of high pollution concentration, high salinity organic waste water, low protein recovery rate, backward processing technology, etc., and is beneficial to comprehensive utilization. , high quality, little effect on quality

Inactive Publication Date: 2015-05-06
林康艺
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are the following problems in the utilization of crustacean processing waste: backward processing technology, high cost, resulting in waste of resources, and a large number of strong acids and alkalis have caused serious pollution, resulting in high-concentration, high-salinity organic wastewater, and its polluting components Contains large amounts of CaCl 2 , NaCl and other salts, a large amount of animal protein (shrimp, crab shell protein) and its degradation products, shrimp and crab shell pigments, oils, impurities brought by the raw materials themselves, a small amount of chitin and chitosan, etc.
However, most of them use a single protease for hydrolysis at present, the hydrolysis efficiency is much lower than the acid-base method, and the protein recovery rate is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Prepare the product with shrimp shells as raw materials. First, dry the shrimp shells at 85°C, then crush the dried shrimp shells to 40 mesh, put them into a beaker and add 6 times of water to mix, put them into an ultrasonic instrument, and adjust the power of the instrument to 250W. Treat for 60min. After the ultrasonic treatment was completed, it was taken out, and it was found that the mixture solution became cloudy and the structure of the shrimp shell changed. Then, the mixture solution was allowed to stand and swell at normal temperature for 1 hour; then, it was heated to 50° C., and a compound enzyme was added in a percentage of 2% by weight according to the weight of the shrimp shell powder raw material put in, and the compound enzyme was composed of 8 parts of trypsin , 10 parts of papain, 7 parts of alkaline protease and 22 parts of subtilisin. Adjust the pH value to about 7.5, stir and react for 2 hours, and filter with a plate and frame filter press to obt...

Embodiment 2

[0020] Prepare the product with shrimp shells as raw materials. First, dry the shrimp shells at 75°C, then crush the dried shrimp shells to 60 mesh, put them into a beaker and add 6 times of water to mix, put them into an ultrasonic instrument, and adjust the power of the instrument to 350W. Treat for 60min. After the ultrasonic treatment was completed, it was taken out, and the mixture solution was found to be cloudy, and the structure of the shrimp shell changed. Then, the mixture solution was allowed to stand and swell at normal temperature for 30 minutes; then, it was heated to 50° C., and a compound enzyme was added in a proportion of 2.3% by weight according to the weight of the shrimp shell powder raw material input, and the compound enzyme was composed of 3 parts of trypsin, Consists of 12 parts papain, 7 parts alkaline protease and 25 parts subtilisin. Adjust the pH value to about 7.8. After stirring and reacting for 2 hours, filter with a plate and frame filter ...

Embodiment 3

[0022] Step and method are identical with embodiment 2, take crab shell as raw material, and its product quality is identical.

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Abstract

The invention belongs to the technical field of natural product separation extraction, and concretely discloses an enzyme-process extraction technology for chitin. The technology comprises the following steps: drying and crushing recovered shrimp and crab shells for usage; adding water with the amount being 6 times by weight of the shrimp and crab powder, performing ultrasonic processing, and standing for swelling; then adding compound-enzyme hydrolyzed protein according to 2-5% of the weight of the shrimp and crab powder, and performing coarse filtration, centrifugal separation and fine filtration, so as to obtain a clean shell powder; and adding a citric acid solution into the obtained clean shell powder for immersing and removing calcium, and performing filtration, cleaning and drying, so as to obtain the chitin product. The compound enzyme is composed of the following compositions in parts by weight: 3-10 parts of trypsin, 10-12 parts of papain, 5-8 parts of alkali protease and 20-30 parts of bacillus subtilis protease. Through ultrasonic pretreatment and proportioning of specific proteases, the protein recovery rate is improved, produced chitin is high in quality, the usage amount of an acid solution in subsequent process of performing acid processing on chitin is substantially reduced, and comprehensive utilization of resource is facilitated.

Description

technical field [0001] The invention belongs to the technical field of separation and extraction of natural products, and relates to a technology for processing and producing chitin by using crustacean processing waste as a raw material, and more specifically, relates to an enzymatic extraction process for chitin. Background technique [0002] my country is rich in crustacean resources, the amount of breeding and processing of crustaceans is huge, and there is a large amount of processing waste at the same time. The waste of crab and shrimp shells alone reaches more than 6,000 tons per year, and the content of chitin (Chitin) in crustacean processing waste is as high as 20~25%. At present, it has been found that chitin and its derivatives have many excellent properties and are widely used in medicine, agriculture, environmental protection, food, textile, printing and dyeing, plastics, color film and other industries. Especially in the pharmaceutical and health care products ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
Inventor 林康艺
Owner 林康艺
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