Enzyme-process extraction technology for chitin

Abstract

The invention belongs to the technical field of natural product separation extraction, and concretely discloses an enzyme-process extraction technology for chitin. The technology comprises the following steps: drying and crushing recovered shrimp and crab shells for usage; adding water with the amount being 6 times by weight of the shrimp and crab powder, performing ultrasonic processing, and standing for swelling; then adding compound-enzyme hydrolyzed protein according to 2-5% of the weight of the shrimp and crab powder, and performing coarse filtration, centrifugal separation and fine filtration, so as to obtain a clean shell powder; and adding a citric acid solution into the obtained clean shell powder for immersing and removing calcium, and performing filtration, cleaning and drying, so as to obtain the chitin product. The compound enzyme is composed of the following compositions in parts by weight: 3-10 parts of trypsin, 10-12 parts of papain, 5-8 parts of alkali protease and 20-30 parts of bacillus subtilis protease. Through ultrasonic pretreatment and proportioning of specific proteases, the protein recovery rate is improved, produced chitin is high in quality, the usage amount of an acid solution in subsequent process of performing acid processing on chitin is substantially reduced, and comprehensive utilization of resource is facilitated.

Description

technical field [0001] The invention belongs to the technical field of separation and extraction of natural products, and relates to a technology for processing and producing chitin by using crustacean processing waste as a raw material, and more specifically, relates to an enzymatic extraction process for chitin. Background technique [0002] my country is rich in crustacean resources, the amount of breeding and processing of crustaceans is huge, and there is a large amount of processing waste at the same time. The waste of crab and shrimp shells alone reaches more than 6,000 tons per year, and the content of chitin (Chitin) in crustacean processing waste is as high as 20~25%. At present, it has been found that chitin and its derivatives have many excellent properties and are widely used in medicine, agriculture, environmental protection, food, textile, printing and dyeing, plastics, color film and other industries. Especially in the pharmaceutical and health care products ...

AI Technical Summary

Problems solved by technology

[0003] At present, there are the following problems in the utilization of crustacean processing waste: backward processing technology, high cost, resulting in waste of resources, and a large number of strong acids and alkalis have caused serious pollution, resulting in high-concentration, high-salinity organic wastewater, and its polluting components Contains large amounts of CaCl 2 , NaCl and other salts, a large amount of animal protein (shrimp, crab shell protein) and its degradation products, shrimp and crab shell pigments, oils, impurities brought by the raw materials themselves, a small amount of chitin and chitosan, etc.

However, most of them use a single protease for hydrolysis at present, the hydrolysis efficiency is much lower than the acid-base method, and the protein recovery rate is not high