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A method for simultaneously detecting the main components of Zhongnaoxintong Capsules in plasma

A technology of Naoxintong and capsules, which is applied in the field of medicine and can solve problems that have not been reported

Active Publication Date: 2016-08-24
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no literature reporting on the pharmacokinetics of these ingredients at the same time, and there are no reports on the basic research on the pharmacodynamic substances of Naoxintong Capsules.

Method used

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  • A method for simultaneously detecting the main components of Zhongnaoxintong Capsules in plasma
  • A method for simultaneously detecting the main components of Zhongnaoxintong Capsules in plasma
  • A method for simultaneously detecting the main components of Zhongnaoxintong Capsules in plasma

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Example 1. Establishment of an analysis method for the main components of the plasma sample Zhongnaoxintong Capsules

[0088] 1.1 LC-MS / MS conditions:

[0089] The chromatographic column is an Agilent Eclipse Plus C18 column (4.6mm×100mm, 1.8μm); the guard column is an Agilent C18 column (2.1×12.5mm, 5μm); the mobile phase is: A: acetonitrile, B: water (containing 0.1% volume fraction formic acid); the flow rate is 300 μL / min; the column temperature is 30°C; the injection volume is 10 μL; the time is 0-30min;

[0090] Gradient elution was adopted, and the elution program is shown in Table 1.

[0091] Table 1 Mobile phase gradient elution program

[0092]

[0093] Mass spectrometry conditions: using API 3200 TM LC / MS / MS liquid mass analysis system; Ion source, positive and negative ion fast switching analysis mode, MRM scanning mode;

[0094] Positive ion mode: curtain gas (CUR): 10psi; collision gas (CAD): 8psi; ion spray voltage (IS): 5000V; temperature (TEM):...

Embodiment 2

[0148] Embodiment 2, pharmacokinetics of main components of Hounaoxintong Capsules after intragastric administration to rats

[0149] 2.1 Collection of plasma samples

[0150] Tested male SD rats were pre-adapted for one week, fasted without food and water for 12 hours the night before the test, and on the second day, the experimental animals were randomly divided into two groups, with 8 rats in each group, and fed with 0.5g / kg and 1.5g / kg respectively. Gastric administration, blood was collected from the fundus venous plexus at 0.083, 0.167, 0.25, 0.5, 0.75, 1, 1.5, 2, 4, 8, 12 and 24 hours after administration, placed in a heparinized centrifuge tube, centrifuged at 8000g for 10 minutes, and taken out The upper layer of plasma was stored in a refrigerator at -20°C until testing.

[0151] 2.2 Pretreatment of plasma samples

[0152] Take 100 μL of plasma sample, accurately add 10 μL of internal standard mixed solution, 10 μL of formic acid, vortex for 1 min, then add 400 μL ...

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Abstract

The invention provides a method for simultaneously detecting main components of paeoniflorin, beta ecdysterone, laetrile, mulberroside A, caffeic acid, ferulic acid, salvianolic acid B, astragaloside, formononetin, cryptotanshinone and tanshinone IIA of a Naoxintong capsule in a plasma sample by a liquid chromatography-tandem mass spectrometry (HPLC-MS / MS). In a liquid chromatogram, a mobile phase consists of acetonitrile and a formic acid aqueous solution of which the volume fraction is 0.1%, and gradient elution is used. A mass spectrum uses a quick positive and negative ions switching and analyzing mode and an MRM (Multiple Reaction Monitoring) scanning manner. After the Naoxintong capsule is taken, the situations of the changes of the blood-medicine concentration of several kinds of main components in the plasma of a rat are detected at the same time. The methodological survey results indicate that the established method conforms to determination requirements on biological samples in a body; the method is good in sensitivity, high in specificity, stable, reliable, and suitable for detecting substances with lower contents.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a method for simultaneously detecting main components of plasma Zhongnaoxintong capsules and its application in pharmacokinetics. Background technique [0002] Naoxintong capsule is exclusively produced by Shaanxi Buchang Pharmaceutical Co., Ltd., and its national drug approval number is Z20090527. Naoxintong Capsules are composed of astragalus, red peony root, salvia miltiorrhiza, angelica, chuanxiong, peach kernel, safflower, frankincense (made), myrrh (made), milletus, achyranthes bidentata, cassia twig, mulberry branch, earthworm, whole Scorpion, leech, a listed Chinese patent medicine composed of sixteen traditional Chinese medicines. Clinically, it is mainly used for apoplexy caused by qi deficiency and blood stagnation, vein stasis, meridians, hemiplegia, numbness of limbs, crooked mouth and eyes, strong tongue, lingering speech, chest pain, chest tightness, palpitatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 常艳旭赵步长李晋赵涛赵菁赵超赵骅高秀梅白洋何俊王虹
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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