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A kind of sulfate-reducing bacteria immobilized particle and its preparation and use method

A sulfate, particle technology, applied in chemical instruments and methods, immobilized on or in inorganic carriers, immobilized on/in organic carriers, etc., can solve the problems of low pH, utilization rate of less than 30%, etc., To achieve the effect of reducing the COD value of effluent, improving the treatment capacity and improving the utilization rate of carbon sources

Active Publication Date: 2018-07-24
LIAONING TECHNICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, due to the constraints of my country's extensive economic development model, about 3 billion m3 of acidic wastewater can be produced every year. 3 , while the utilization rate is less than 30%, far lower than the 80% standard in developed countries such as Europe and the United States
Although sulfate-reducing bacteria have great potential in acid wastewater treatment, they also expose many shortcomings, such as low pH, high concentration of heavy metal ion inhibition, and the need for continuous carbon source dosing to provide nutrients for bacteria, etc.

Method used

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  • A kind of sulfate-reducing bacteria immobilized particle and its preparation and use method
  • A kind of sulfate-reducing bacteria immobilized particle and its preparation and use method
  • A kind of sulfate-reducing bacteria immobilized particle and its preparation and use method

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preparation example Construction

[0037] The preparation method of the sulfate-reducing bacteria sludge stock solution is as follows: sulfate-reducing bacteria raw materials such as municipal activated sludge, landfill leachate sludge, and coal gangue polluted soil containing sulfate-reducing bacteria and hydrolytic microorganisms are diluted 10-20 times Take out 25mL-50mL and add it to 500mL-1000mL improved Starkey-type culture medium, and anaerobic culture at a constant temperature of 37±1℃ for several weeks to form sulfate-reducing bacteria as the dominant bacteria in the sludge, including hydrolytic microorganisms A mixed sulfate-reducing bacteria sludge floc that coexists with a variety of microbial strains; when the bottle cap is opened after cultivation, a strong smell of rotten eggs can be smelled, and the sludge suspension is dripped into the 2+ When the solution can immediately turn the solution into ink color, the sludge stock solution of sulfate-reducing bacteria is obtained;

[0038] The compositi...

Embodiment 1

[0041] A sulfate-reducing bacteria immobilized particle, the weight percentage of each component is: 30% sulfate-reducing bacteria sludge, 2% steel slag powder, 3% modified medical stone, 9% polyethylene alcohol, 0.5% sodium alginate, 5% corn cob and 50.5% water.

[0042] Its preparation method is:

[0043] Choose steel slag powder with a particle size of 80 mesh, wash it 3 times with 1mol / L HCl, and air-dry it; choose ordinary medical stone powder with a particle diameter of 60 mesh, use 2mol / L NaOH solution and medical stone powder according to the volume-to-mass ratio of 1:5kg / L mixed, soaked for 1 hour, then oscillated at 150r / min for 3 hours, then took out the medical stone, soaked it with deionized water for 3 times, baked at 300°C for 90 minutes, and cooled naturally to obtain Modified medical stone; corn cobs are dried in the sun and crushed mechanically to make a powder with a particle size of 100 mesh;

[0044] Add 9g of polyvinyl alcohol and 0.5g of sodium alginat...

Embodiment 2

[0050] A sulfate-reducing bacteria immobilized particle, the weight percentage of each component is: 20% sulfate-reducing bacteria sludge, 6% steel slag powder, 10% medical stone, 5% polyvinyl alcohol, 0.2% sodium alginate, 4% peanut shells and 54.8% water.

[0051] Its preparation method is:

[0052] Select steel slag powder with a particle size of 90-100 mesh, wash 2-3 times with 2mol / L HCl, and air-dry; select ordinary medical stone powder with a particle size of 70-80 mesh, wash with water 2-3 times, and air-dry; take peanut shells After drying, it is mechanically crushed to make a powder with a particle size of 80-100 mesh;

[0053] Add 5g of polyvinyl alcohol and 0.2g of sodium alginate into 54.8g of distilled water, fully swell at room temperature for 20 hours after sealing, then heat in a constant temperature water bath at 94±1°C, and keep stirring until there are no bubbles in the gel ;

[0054] Then slowly add 4g of biomass material peanut shell powder, 6g of stee...

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Abstract

The invention aims to providing a sulfate reducing bacteria immobilization granule as well as preparation and use methods thereof, aiming at the defects of sulfate reducing bacteria in acid wastewater treatment, and belongs to the technical field of water pollution control engineering. The sulfate reducing bacteria immobilization granule comprises the following materials in percentage by weight: 20%-45% of sulfate reducing bacteria cement, 2%-8% of steel slag powder, 3%-10% of modified medical stones, 5%-9% of polyvinyl alcohol, 0.2%-1.5% of sodium alginate, 4%-15% of biologic materials and 11.5%-65.8% of water. The sulfate reducing bacteria immobilization granule provided by the invention prevents the direct contact of the sulfate reducing bacteria and acid wastewater by adopting a polyvinyl alcohol-boric acid embedding method on the basis of a microbial immobilization technology, thereby solving the problem of inhibition of strong acid and high-concentration heavy metals on the sulfate reducing bacteria; and the sulfate reducing bacteria immobilization granule provided by the invention can carry out downhole in-situ treatment on the acid wastewater of a coal mine and be recyclable, can be used for effectively overcoming the defects of the traditional technology and improving the ecological quality of a mine area and is a passive processing system which is free of occupying ground space or additionally increased in power.

Description

technical field [0001] The invention belongs to the technical field of water pollution control engineering, and in particular relates to sulfate-reducing bacteria immobilized particles for treating acidic wastewater in coal mines and a preparation and use method thereof. Background technique [0002] As the most important energy variety, coal has always accounted for about 70% of my country's primary energy consumption structure. Coal enterprises have made important contributions to the sustained high growth of my country's macro economy in the past 30 years of reform and opening up. However, coal resources are also being developed and processed. A large amount of coal mine wastewater is produced in the process of transportation and utilization. At present, due to the constraints of my country's extensive economic development model, about 3 billion m3 of acidic wastewater can be produced every year. 3 , while the utilization rate is less than 30%, far lower than the 80% stan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N11/08C02F3/34
Inventor 狄军贞朱志涛鲍娟柴博郭旭颖张丽刚江富戴男男赵前程任亚东安文博
Owner LIAONING TECHNICAL UNIVERSITY
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