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Method for rapidly cultivating calculus bovis

A bezoar and rapid technology, applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, peptide/protein components, etc., can solve the problems of long production cycle, long production cycle, inconsistent product quality, etc., and achieve short production cycle and safety Effect with few side effects

Inactive Publication Date: 2015-06-03
毛泽春 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that the generation period is as long as 2-3 years, the stone production rate is low, the product quality is inconsistent, and it needs to kill cattle to get stones
[0026] 1) Long production cycle

Method used

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  • Method for rapidly cultivating calculus bovis
  • Method for rapidly cultivating calculus bovis
  • Method for rapidly cultivating calculus bovis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0145] Embodiment 1 (as figure 2 shown):

[0146] Step 1: Obtain 1000ml of fresh ox bile with aseptic technique and place it in a sterile tank; add the following substances to other sterile containers filled with 1000ml of distilled water to prepare a mixed solution for later use:

[0147]

[0148] Step 2: Obtain the plasma thawed sediment as follows:

[0149] 1) Take 100 ml of sodium citrate solution with a concentration of 3.2%, put it into 900 ml of fresh bovine blood, and mix well;

[0150] 2) With a centrifugal force of 1500g, centrifuge the above anticoagulated whole blood for 15-20 minutes to make plasma;

[0151] 3) Freeze the above plasma at -5°C for more than 30 minutes, and then thaw at 4°C;

[0152] 4) After the thawing is completed, the thawed plasma is immediately centrifuged with a centrifugal force of 1000g, and the thawed plasma containing fibrinogen is separated;

[0153] 5) detection of fibrinogen concentration;

[0154] 6) Adjust the concentration ...

Embodiment 2

[0166] Embodiment 2 (as image 3 shown):

[0167] In this embodiment, except that the mixed solution and mixture ratio in steps 1 and 3 described in embodiment 1 are different from those in embodiment 1 and the contents in steps 4, 5 and 6 are different, the rest of the steps are the same as in embodiment 1.

[0168] The formula of the mixed solution in the present embodiment is as follows:

[0169]

[0170] Dissolve in 1000ml of distilled or deionized water for later use.

[0171] The mixture formula in the present embodiment is as follows:

[0172]

[0173]

[0174] In this example:

[0175] Step 4: Take 100 ml of bovine thrombin of 10 IU / ml and place it in the aseptic jar.

[0176] Step 5: Add 500ml of 0.03M PBS and the spare mixture to the sterile tank, adjust the pH to 7.3, stir to form a suspension, and pre-incubate at 37°C for 5 minutes.

[0177] Step 6: Add 1,700 ml of pre-separated plasma thawed sediment containing 0.06% (W / V) bovine fibrinogen to the su...

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Abstract

The invention relates to a method for rapidly cultivating calculus bovis which is low in cost, short in preparation cycle, stable in performance and little in side effect. The method comprises the step: obtaining natural ox bile and further comprises the following steps: (1) preparing a basic stock solution containing fibrinogen and other blood coagulation factors; (2) preparing a mixture containing deoxycholic acid, cholic acid, calcium bilirubin, cholesterol, phospholipid and calcium chloride for later use; (3) preparing mixed liquor containing taurine and trace amounts of FeSO4, MgSO4 and ZnSO4 for later use; (4) taking fresh ox bile for later use; (5) preparing an ox gall-stone sediment according to a set process; (6) separating, shaping and drying to obtain ox gall-stones. The method has the advantages that 1, the production cycle is short; 2, the method is safe and little in side effect; 3, the concentration of free bilirubin is more easily controlled; 4, the generated ox gall-stones are complete, the effective ingredients and the biological activity are better. The preparation principle and the reaction condition and process belong to a new method, and the quality of the generated calculus bovis is more stable than that of the natural calculus bovis.

Description

technical field [0001] The invention relates to a preparation method of famous and precious traditional Chinese medicine raw materials, in particular to a preparation method of cultivated bezoar, an in vitro substitute of natural bezoar. Background technique [0002] Natural bezoar is dry bovine pigmented gallstones. Since ancient times, natural bezoar has been a kind of famous and precious Chinese medicinal materials and raw materials. It has the effects of clearing heat and detoxifying, cooling the liver and calming the wind, clearing the heart and resuscitation. It is mainly used for the treatment of high fever, coma, epileptic convulsions, apoplexy, phlegm loss, aphtha of the mouth and other diseases. It can be administered directly to patients, or it can be used as a raw material for more than 600 kinds of Chinese patent medicines. The most important ones include Angong Niuhuang Pills, Anshen Pills, Pien Tze Huang and other expensive medicines. But natural calculus ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/413A61K38/36A61K38/00A61K31/575A61K31/409
Inventor 毛泽春关丽云
Owner 毛泽春
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