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Polyurethane material for adsorbing TGF-B1 growth factor for tissue repair and preparation method thereof

A polyurethane material, TGF- technology, applied in medical science, prosthesis and other directions, can solve the problems of complex preparation method, achieve the effect of simple and convenient preparation process, improve tissue repair effect, and reduce release speed

Inactive Publication Date: 2015-06-03
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation method is complicated

Method used

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  • Polyurethane material for adsorbing TGF-B1 growth factor for tissue repair and preparation method thereof
  • Polyurethane material for adsorbing TGF-B1 growth factor for tissue repair and preparation method thereof
  • Polyurethane material for adsorbing TGF-B1 growth factor for tissue repair and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Weigh 20g of PCL-2000 in a three-necked flask, dehydrate under vacuum at 100°C for 30min (1mbar), add 100ml of DMF, 3.36g of HDI and 0.005g of stannous octoate catalyst under a nitrogen atmosphere, then cool down to 80°C for 2.5 h to form a prepolymer. After the prepolymer was formed, 1.52 g of chain extender DPPD was added. After raising the reaction temperature to 110°C, the reaction was continued for 4 hours, and water was added to terminate the reaction. After drying, it was purified by co-precipitation with methanol to obtain an alkyne-containing polyurethane. Finally, in the mixed solution of anhydrous copper sulfate (2mol%) and sodium ascorbate (10mol%), the azide-modified HSNGLPL is grafted to the surface of polyurethane synthesized by "click chemistry" reaction, and the system ambient oxygen concentration≤10ppm, the reaction The time is 12 hours, and a surface-modified polyurethane material is obtained, which can specifically adsorb TGF-β1 growth factor.

[0...

Embodiment 2

[0044]Weigh 5g of PEG-500 in a three-necked flask, dehydrate at 100°C for 30min under vacuum (1mbar), add 20ml of DMF, 2.75g of MDI and 0.2g of stannous octoate catalyst under nitrogen atmosphere, then cool down to 60°C for 4h to form a prepolymer. After the prepolymer was formed, 0.15 g of DPPD was added. Continue to react at 60°C for 24h, add water to terminate the reaction, and purify with methanol co-precipitation after drying. Finally, in the mixed solution of anhydrous copper sulfate (5mol%) and sodium ascorbate (10mol%), the azide-modified HSNGLPL is grafted to the surface of the synthetic polyurethane by "click chemistry" reaction, the system ambient oxygen concentration≤10ppm, the reaction time After 36 hours, a functionalized polyurethane material that specifically adsorbs TGF-β1 growth factor was obtained.

[0045] The performance of the functionalized polyurethane material specifically adsorbing TGF-β1 growth factor obtained in this example is similar to that of ...

Embodiment 3

[0047] Weigh 10g of PTMG-1000 in a three-necked flask, dehydrate at 100°C for 30min (1mbar) under vacuum, add 50ml of DMF, 5.22g of TDI and 0.005g of triethylamine catalyst under nitrogen atmosphere, then cool down to 90°C for 1h to form a prepolymer. After the prepolymer was formed, 3.04 g of DPPD was added. After raising the reaction temperature to 100° C., the reaction was continued for 5 h, and water was added to terminate the reaction. After drying, it was purified by co-precipitation with methanol. Finally, the azide-modified HSNGLPL was grafted onto the surface of the synthesized polyurethane by "click chemistry" reaction. A functionalized polyurethane material capable of specifically adsorbing TGF-β1 growth factor is obtained.

[0048] The performance of the functionalized polyurethane material specifically adsorbing TGF-β1 growth factor obtained in this example is similar to that of Example 1.

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Abstract

The invention discloses a polyurethane material for adsorbing a TGF-B1 growth factor for tissue repair and a preparation method thereof. The preparation method comprises the following steps: (1) reacting the polymer dihydric alcohol, diisocyanate and a catalyst for 0.5-24h at 60-120 DEG C to form a prepolymer; (2) adding an alkynyl chain extender into the prepolymer, reacting for 0.5-24h at 60-120 DEG C, adding water to end the reaction, baking and purifying through a coprecipitation method to obtain the functional polyurethane; and (3) performing click reaction on the nitrine modified HSNGLPL affinity peptide and the functional polyurethane in the mixed solution of the anhydrous cupric sulfate and the sodium ascorbate. The alkynyl chain extender is introduced into the polyurethane and is combined with the HSNGLPL affinity peptide through the click chemistry. The preparation method is simple and convenient, and the obtained product can specifically adsorb the TGF-B1 growth factor.

Description

technical field [0001] The invention relates to the field of preparation of tissue repair materials, in particular to a polyurethane material for tissue repair that absorbs TGF-β1 growth factor and a preparation method. Background technique [0002] The process of tissue regeneration and repair is a biological process regulated by a variety of cytokines and growth factors, among which the transforming growth factor (TGF) β family plays an important role in the regulation of cell growth, inflammatory response, extracellular matrix formation and cell apoptosis. important role. Currently, TGF-β1 is the growth factor most studied in cartilage repair, which plays an important role in embryonic cartilage formation and cartilage callus formation in fracture healing. Therefore, TGF-β1 plays an important role in inducing cartilage differentiation and maintaining cartilage phenotype. [0003] Due to the lack of tissues such as nerves, blood vessels, and lymphatic vessels, adult chon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G18/83C08G18/67C08G18/10A61L27/18
CPCC08G18/833A61L27/18A61L27/54A61L2300/414C08G18/10C08G18/679
Inventor 吴刚肖梦陈龙郭琳黄超孙佳维
Owner SOUTH CHINA UNIV OF TECH
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