Homocysteine (HCy) kit based on aptamer fluorescence probe and detection method for concentration of homocysteine (HCy) by using homocysteine (HCy) kit

A homocysteine ​​and fluorescent probe technology, applied in the field of homocysteine ​​HCy kits, can solve the problems of operational technical errors, poor repeatability, and many interference factors, achieving high repeatability and convenient transportation , strong specific effect

Inactive Publication Date: 2015-06-03
陈燕婷 +2
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a homocysteine ​​HC based on nucleic acid aptamer fluorescent probe for the existing deficiencies such as easy to produce operational technical errors, poor repeatability, many interference factors, cumbersome operation, and high testing cost. y Reagent kit and detection method thereof

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Homocysteine (HCy) kit based on aptamer fluorescence probe and detection method for concentration of homocysteine (HCy) by using homocysteine (HCy) kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] After dissolving and preparing the required reagent components according to Example 1 in Table 1, divide into bottles and freeze-dry to make a dry powder reagent; before use, add ultrapure water and use after reconstitution. Three parallels were set for each sample, and the detection steps were as follows:

[0035] (1) Lysis of blood samples: Mix heparin anticoagulated whole blood and erythrocyte lysate at a volume ratio of 1:0.5, let stand for 30 minutes, then centrifuge at a medium speed for 7 minutes, and collect the supernatant;

[0036] (2) Mixed egg incubation: take 20 μl of the supernatant obtained in step 1) and 45ul of the homocysteine ​​nucleic acid aptamer fluorescent probe reagent obtained by dissolving the homocysteine ​​nucleic acid aptamer fluorescent probe in 0.2M phosphate buffer Mix and incubate the eggs at room temperature for 5 minutes to obtain the test solution;

[0037] (3) Fluorescence detection: 50ul of the test solution obtained in the detecti...

Embodiment 2

[0041] After dissolving and preparing the required reagent components according to Example 2 in Table 1, divide into bottles and freeze-dry to make a dry powder reagent; before use, add ultrapure water and use after reconstitution. Three parallels were set for each sample, and the detection steps were as follows:

[0042] (1) Lysis of blood samples: Mix peripheral blood and erythrocyte lysate at a volume ratio of 1:2.5, let stand for 5 minutes, then centrifuge at a medium speed for 6 minutes, and collect the supernatant;

[0043] (2) Mixed egg incubation: take 50 μl of the supernatant obtained in step 1) and 30ul of

[0044] The homocysteine ​​aptamer fluorescent probe reagent obtained by dissolving the homocysteine ​​nucleic acid aptamer fluorescent probe in 0.2M phosphate buffer was mixed, and the eggs were incubated at room temperature for 6 minutes to obtain the test solution;

[0045] (3) Fluorescence detection: 50ul of the test solution obtained in the detection step 2)...

Embodiment 3

[0048] After dissolving and preparing the required reagent components according to Example 3 in Table 1, divide into bottles and freeze-dry to make a dry powder reagent; before use, add ultrapure water and use after reconstitution. Three parallels were set for each sample, and the detection steps were as follows:

[0049] (1) Lysis of blood samples: Mix peripheral blood and erythrocyte lysate at a volume ratio of 1:3.5, let stand for 10 minutes, then centrifuge at a medium speed for 5 minutes, and collect the supernatant;

[0050] (2) Mixed egg incubation: take 75 μl of the supernatant obtained in step 1) and 45ul of

[0051] The homocysteine ​​aptamer fluorescent probe reagent obtained by dissolving the homocysteine ​​nucleic acid aptamer fluorescent probe in 0.2M phosphate buffer was mixed, and the eggs were incubated at room temperature for 7 minutes to obtain the test solution;

[0052] (3) Fluorescence detection: 50ul of the test solution obtained in the detection step 2...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
Login to view more

Abstract

The invention relates to a homocysteine (HCy) kit based on an aptamer fluorescence probe and a method for measuring the concentration of homocysteine (HCy) as well as the composition and components of a measuring reagent, and belongs to the technical field of medical examination and measurement. The kit provided by the invention mainly comprises erythrocyte lysate, a phosphate buffer solution PBS, a standard sample of homocysteine and the homocysteine aptamer fluorescence probe. The concentration of the homocysteine is measured through blood sample cracking and mixed incubation treatment in combination with fluorospectrophotometer detection. The kit and the method provided by the invention have the advantages of easiness in sample treatment, easiness in operation, short detection time, high detection specificity, high sensitivity, high repeatability of detection result, and the like.

Description

technical field [0001] The invention belongs to the technical field of medical examination and measurement, in particular to a homocysteine ​​HCy kit based on a nucleic acid aptamer fluorescent probe and a detection method thereof. Background technique [0002] Homocysteine ​​is a sulfur-containing amino acid and an intermediate product of methionine metabolism in organisms. Compared with cysteine, there is one more methylene group in the side chain. Homocysteine ​​is a risk factor for many diseases. At present, the increase of homocysteine ​​is considered to be the most extensive and independent pathogenic factor of cardiovascular and cerebrovascular diseases caused by atherosclerosis. Homocysteine ​​is also related to various diseases such as neural tube defects, Parkinson's disease, senile dementia, and chronic renal failure, as well as the effects of certain drugs and tumors. The main methods for detecting homocysteine ​​are chromatographic methods, immunological method...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 陈燕婷张莘蔓李红梅
Owner 陈燕婷
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap