Agarose microsphere-containing cross-linked sodium hyaluronate gel for injection and preparation method thereof

A technology for cross-linking hyaluronic acid and agarose microspheres, which is applied in the fields of medical beauty and biomedicine, can solve the problems of increasing the burden on consumers and high cost of use by consumers, and achieves the effect of long-lasting shaping and prolonging the degradation cycle.

Inactive Publication Date: 2015-07-15
JIANGYIN BAIYU TIANGU BIOLOGICAL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current sol products have the following defects: 1. The cost of use by consumers is relatively high, and the cost of each injection can be thousands or even tens of thousand

Method used

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  • Agarose microsphere-containing cross-linked sodium hyaluronate gel for injection and preparation method thereof
  • Agarose microsphere-containing cross-linked sodium hyaluronate gel for injection and preparation method thereof
  • Agarose microsphere-containing cross-linked sodium hyaluronate gel for injection and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] First add 0.1g of agarose microspheres and 10mL of water for injection into a clean beaker with a volume of 50mL. After simple stirring, add 2.5g of injection-grade sodium hyaluronate powder, and stir until the sodium hyaluronate is completely swollen. Add 15mL of sodium hydroxide solution prepared with water for injection and 1.2g of 1,2,7,8-dioxoctane (DEO) to the above-mentioned swollen sodium hyaluronate solution, and stir carefully until the final hydrogen The concentration of sodium oxide is 0.3mol / L. Then the evenly stirred mixture was placed in water at 50°C and heated for 4 hours to obtain a pale yellow gel with certain elasticity. Then cut the obtained gel into small pieces with a scalpel blade, wash with 0.05mol / L dilute hydrochloric acid at room temperature for 5 hours, and start washing with water for injection after washing. Once, the injection water was changed 10 times in total. Pass the washed gel twice through a sieve made of 35-mesh stainless steel ...

Embodiment 2

[0027] First add 0.5g of agarose microspheres and 15mL of water for injection into a clean beaker with a volume of 50mL, and then add 1.5g of injection-grade sodium hyaluronate powder after simple stirring, and stir until the sodium hyaluronate is completely swollen; Then add 10mL of sodium hydroxide solution prepared with water for injection and 0.6g of 1,2,7,8-diepoxyoctane (DEO) to the sodium hydroxide solution, and stir carefully until the final sodium hydroxide concentration is 0.1mol / L, place the evenly stirred mixture in water at 40°C and heat for 10 hours to obtain a pale yellow gel with certain elasticity. Cut the obtained gel into small pieces with a scalpel, wash with 0.05mol / L dilute hydrochloric acid at room temperature for 5 hours, and then change to water for injection to start washing at a washing temperature of 40°C, changing the water once every 1 hour, and changing the water 10 times in total ; Pass the washed gel through a 35-mesh stainless steel screen tw...

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Abstract

The invention discloses agarose microsphere-containing cross-linked sodium hyaluronate gel for injection and a preparation method thereof. The preparation method comprises the following steps of 1, adding agarose microspheres into injection water, carrying out stirring, adding injection-level sodium hyaluronate powder into the mixed solution, and carrying out stirring until complete swelling, 2, adding a sodium hydroxide solution and 1,2,7,8-diepoxyoctane into the solution obtained by the step 1, and carrying out stirring under control of a sodium hydroxide concentration, 3, heating the mixture to obtain water-containing gel, 4, cutting the water-containing gel into small pieces and carrying out washing, 5, sieving the washed gel pieces, draining water in the gel pieces and adding a stroke-physiological saline solution into the gel pieces for balance, and 6, carrying out centrifugation and disinfection to obtain the agarose microsphere-containing cross-linked sodium hyaluronate gel finished product. The agarose microsphere-containing cross-linked sodium hyaluronate gel utilizes medical sodium hyaluronate, medical agarose microspheres, endotoxin, other proteins and exogenous DNA, and has indexes satisfying national and industry relevant standards. Through use of the agarose microspheres, the gel has a substantially slow resolving rate after injection and is an ideal injection filling product.

Description

technical field [0001] The invention belongs to the technical fields of medical cosmetology and biomedicine, and in particular relates to a cross-linked sodium hyaluronate gel containing agarose microspheres for subcutaneous and dermal injection and a preparation method thereof. Background technique [0002] Injection cosmetology is a kind of non-surgical plastic surgery. It uses injection method to inject biomaterials or synthetic materials with good biocompatibility into the dermis or subcutaneous layer, and achieves the purpose of reducing skin wrinkles or shaping through different mechanisms. A method of plastic surgery. Sodium hyaluronate is a polysaccharide biomaterial with very good biocompatibility. The degradation and metabolism process in the body has been studied clearly and is safe for the human body. It is the first choice for the preparation of gel products. Nowadays, gel products are well known to the public as an important product in the field of micro-plast...

Claims

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Application Information

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IPC IPC(8): C08J3/24C08J3/075C08L5/08C08L5/12C08K5/1515A61L27/60A61L27/52A61L27/20
Inventor 顾丽峰
Owner JIANGYIN BAIYU TIANGU BIOLOGICAL PHARMA
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