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Layer-by-layer-self-assembling small-caliber tissue engineering blood vessel and establishing method thereof

A layer-by-layer self-assembly and tissue engineering technology is applied in the field of bioengineering to achieve the effects of simple production, good biocompatibility, and good cell induction

Inactive Publication Date: 2015-07-22
广州宏畅生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no controlled release system that is safe and stable to control the release of adenosine, and is suitable for the special environment of the blood circulation system.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0013] 1. Preparation of sustained-release nanoparticles: 80-100 mg of β-cyclodextrin and 20-30 mg of adenosine were dissolved in 20-30 ml of deionized water, and stirred at room temperature for 24-48 hours. Dissolve 60-80mg of chitosan in 1% glacial acetic acid solution, stir evenly, slowly add 1-2ml of 1-3% TPP solution, and continue stirring at room temperature for 2-8 hours. Mix chitosan solution, β-cyclodextrin and adenosine solution at a ratio of 3-5:1, continue to stir for 2-4 hours, centrifuge at 20,000g / min at 4 degrees, wash with distilled water three times, and freeze-dry . Scanning electron microscopy was used to detect the morphology of nano-sustained-release particles, and infrared spectroscopy was used to detect its wrapping. The results showed that the nanoparticles contained one of the peaks consistent with the sample scanning peak, indicating that the nanoparticles contained the relevant samples, while the control group did not contain the corresponding peak....

Embodiment 1

[0020] Example 1. A bioartificial blood vessel prepared by in vivo mobilization and capture of endothelial progenitor cells mediated by energy metabolism, the vascular matrix material is prepared according to the above-mentioned step 2 method, and after the drugs such as adenosine are wrapped according to the method of step 1, the 3 After surface modification, animal experiments are carried out.

[0021] The obtained bioartificial blood vessels were respectively transplanted into the carotid arteries of animals. After 14 days of examination by light microscope and electron microscope, it can be seen that the endothelial progenitor cells in the vascular lumen were normal in shape, densely arranged, and distributed along the long axis of the blood vessel, indicating that under the above conditions, the blood vessel was successfully formed. Autologous endothelialization. One month later, HE staining, immunofluorescence staining, and electron microscopy showed that the endothelial...

Embodiment 2

[0022]Example 2. Preparation of a bioartificial blood vessel capable of promoting the mobilization and homing of endothelial progenitor cells by regulating the inflammatory response. The vascular matrix material is prepared according to the above step 2, and the adenosine A2a receptor agonist is wrapped according to the method of step 1. , after performing surface modification according to step 3, then proceed to animal experiments. After 14 days of light and electron microscopy, it can be seen that the vascular endothelial progenitor cells are normal in shape, densely arranged, and distributed along the long axis of the blood vessel, indicating that under the above conditions, the blood vessel has successfully formed its own endothelialization. One month later, HE staining, immunofluorescence staining, and electron microscopy showed that the endothelialization of the blood vessels was good, without significant intimal hyperplasia and thrombus formation, and the distribution of...

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Abstract

The invention relates to a layer-by-layer-self-assembling small-caliber tissue engineering blood vessel and an establishing method thereof. The method mainly comprises the steps that chitosan / beta-cyclodextrin nano slow-release particles are established, and wrapping endothelial progenitor cells are mobilized to capture molecules; natural biological materials or artificial synthetic materials are knitted into artificial blood vessels in an electric spinning method, or decellularization homologous or heterogeneous blood vessels are used; and tissue engineering blood vessel surface modification is carried out, and a layer-by-layer-self-assembling mode is used for modifying molecule nano particles captured and mobilized by wrapping adenosine, receptor stimulant thereof and the like to a biology artificial blood vessel support. The artificial blood vessel can be established conveniently, releasing of medicine can be effectively controlled, medicine sudden releasing can be lowered, and meanwhile good mechanical property and clinic practicability are achieved. Blood vessels of any caliber can be established, and in-vivo induced circulation blood endothelial progenitor cells mobilization and homing can be carried out. Artificial blood vessel endothelialization can be promoted quickly, and meanwhile hemadostenosis and blocking caused by smooth muscle cell pathologic proliferation can be restrained for a long time.

Description

technical field [0001] The invention relates to bioengineering technology, in particular to a nanoparticle-modified layer-by-layer self-assembled bioartificial blood vessel and a construction method thereof. Background technique [0002] In recent years, the morbidity and mortality of cardiovascular diseases have been increasing year by year, and a large number of patients need bypass grafting every year. Therefore, there is a high clinical demand for tissue engineered blood vessels with a diameter of less than 6mm. However, small-diameter tissue-engineered blood vessels often fail due to thrombosis and intimal hyperplasia. A large number of studies have shown that endothelial cells have the functions of antithrombosis, inhibition of platelet aggregation and pathological proliferation of smooth muscle cells. Therefore, early and rapid endothelialization of tissue engineered blood vessels is a very important factor to maintain the patency of small diameter tissue engineered b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/06A61M31/00
Inventor 朱楚洪陈文曾文
Owner 广州宏畅生物科技有限公司
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