A preparing method of a nanometer polymer micelle carrier integrating diagnosis and treatment
A nano-polymer and micelle technology, applied in the field of preparation of bifunctional nano-polymer micelle system, can solve the problems of allergic reaction, toxic reaction and the like, and achieve the effect of improving local concentration, simple operation and easy implementation
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[0041] (4) Preparation of FITC-PEG-PCL
[0042] Add 1-5 parts by weight of NH 2 -PEG-PCL is dissolved in tetrahydrofuran solution, dripped into 5-10 parts by weight of an aqueous solution with PH=10 under ultrasonic conditions to prepare a micellar solution, then 1500-4000 parts by weight of FITC is added, and stirred at room temperature 24-48h, then dialyzed with deionized water (MWO=100kDa), and freeze-dried to obtain FITC-PEG-PCL.
Example Embodiment
[0043] Example 1
[0044] Prepare nano-polymer micelles loaded with anti-tumor drug paclitaxel (PTX) and superparamagnetic iron oxide nanoparticles (SPIO).
[0045] SPIO nanoparticles are encapsulated by self-assembly of amphiphilic block polymer micelles and loaded with the hydrophobic antitumor drug paclitaxel. Add 0.6mg of PTX, Fe 3 O 4 Nanoparticles 6.0mg, 13.5mg MPEG-PCL and 3mg FA-PEG-PCL, 3mg FITC-PEG-PCL were dissolved in 1.5ml of tetrahydrofuran (THF), then the above mixture was added to 15ml of deionized water under ultrasound, and dialyzed 2 -Volatilize THF for 3 days to remove organic solvents and unpackaged anti-tumor drugs to obtain FA-NP / SPIO / PTX nano-polymer micelles. The micelle solution is filtered through a 450nm filter membrane and ultrafiltration centrifuge tube. Centrifuge 2 times at 2500-3000rpm.
[0046] The measurement of the particle size of the FA-NP / SPIO / PTX nano-polymer micelle prepared in Example 1.
[0047] Use dynamic light scattering to measure the p...
Example Embodiment
[0061] Example 2
[0062] Prepare nano-polymer micelles loaded with anti-tumor drug paclitaxel (PTX) and superparamagnetic iron oxide nanoparticles (SPIO).
[0063] SPIO nanoparticles are encapsulated by self-assembly of amphiphilic block polymer micelles and loaded with the hydrophobic antitumor drug paclitaxel. Add 0.1mg of PTX, 1.0mg of CoFe 3 O 4 Nanoparticles, 4mg MPEG-PCL and 0.8mg FA-PEG-PCL, 0.8mg FITC-PEG-PCL were dissolved in 2ml tetrahydrofuran (THF), and then the above mixture was added to 20ml deionized water under ultrasound, and it was evaporated for 3 days after dialysis THF is used to remove organic solvents and unpackaged anti-tumor drugs to obtain FA-NP / SPIO / PTX nano-polymer micelles. The micelle solution is filtered through a 450nm filter membrane and an ultrafiltration centrifuge tube is centrifuged at 3000rpm twice , The prepared core is loaded with hydrophobic drugs and magnetic resonance sensitive molecular probes, and the outer shell is equipped with a nan...
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