Special water-retaining biological organic fertilizer for blueberries and preparation method thereof
A technology of organic fertilizer and aquatic organisms, which is applied in the field of special water-retaining bio-organic fertilizer for blueberries and its preparation, can solve the problems of unfavorable blueberry normal growth and difficult realization of key functions, so as to improve water use efficiency, strong pertinence and effectiveness, Improve the effect of micro-ecological environment
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Embodiment 1
[0044] The preparation of embodiment 1 blueberry special-purpose water-retaining bio-organic fertilizer
[0045]Mix 3 parts of beef extract, 10 parts of peptone, 5 parts of sodium chloride, and 982 parts of water to make a culture medium, and expand Pseudomonas X123 and Bacillus fusiformis L13 in the culture medium to prepare Pseudomonas X123 Fermentation liquid, Bacillus fusiformis L13 fermentation liquid;
[0046] Prepare the fermentation broth of Saccharomyces cerevisiae and Lactobacillus casei according to the culture method provided by the strain preservation unit.
[0047] The obtained fermented liquid is mixed evenly according to the number ratio of the number of viable bacteria: the ratio of the number (cfu) of Pseudomonas X123, Bacillus fusiformis L13, Lactobacillus casei and Saccharomyces cerevisiae is: 40:30:15:15 . The number of viable bacteria in the bacterial liquid of the microbial composition is 6.58×10 8 cfu / ml. Boil soybean sprouts and water at a ratio of...
Embodiment 2
[0052] Embodiment 2 is specially used in the microbial composition bacterium liquid of blueberry
[0053] Mix Pseudomonas X123, Bacillus fusiformis L13, Lactobacillus casei, and Saccharomyces cerevisiae in a cfu ratio of 40:30:15:15, and the number of viable bacteria in the microbial composition liquid is 6.58×10 8 cfu / ml. Plant blueberry seedlings, and mix the 4 kinds of bacterial solutions with water in a ratio of 1:2, and pour in the total amount of 4 billion viable bacteria per pot. After 6 months of blueberry growth, the physical and chemical properties of the blueberry rhizosphere soil were measured, and the plant height and stem diameter were measured. The results are shown in Table 1 below. It can be seen that compared with the single inoculation of Pseudomonas X123, Bacillus fusiformis L13, Lactobacillus casei, and Saccharomyces cerevisiae, the number of bacteria in the rhizosphere soil treated with the microbial composition bacterial solution was significantly highe...
Embodiment 3
[0056] The test of the effective number of viable bacteria of embodiment 3 gel microbial composition
[0057] Soybean sprouts and water were boiled for 10 minutes according to the ratio of 1:30 (g / ml), cooled and filtered, added 1% glucose and 0.3% urea, dissolved and mixed to make a special diluent. Mix Pseudomonas X123, Bacillus fusiformis L13, Lactobacillus casei, and Saccharomyces cerevisiae in a cfu ratio of 40:30:15:15, and the number of viable bacteria in the microbial composition liquid is 6.58×10 8 cfu / ml, dilute with water and special diluent 1:5 (V / V) respectively. Take by weighing 50g of CLP type brake carrier, fully mix the two kinds of microbial composition bacterium liquids after being diluted with the carrier respectively by the ratio of 25:1 (ml / g), make the gel microbial composition, make its Store in a cool, dry place at room temperature. After the carrier is adsorbed, take 10 g of samples, and measure the number of viable bacteria by the dilution plate co...
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