A Microbiological Method for Detecting Heavy Metal Copper in Water

A water body and copper ion technology, applied in the field of environmental biology, can solve the problems of poor stability of fluorescence detection signals and poor repeatability of independent experiments, slow development of heavy metal microbial detection technology, uneven plasmid copy number, etc., and achieve short detection time, Fluorescence intensity value deviation is small, and the effect of improving sensitivity
CN104845998BActive Publication Date: 2019-05-07WENZHOU MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
WENZHOU MEDICAL UNIV
Publication Date
2019-05-07

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Abstract

The invention provides a preparation method for an engineering strain of Escherichia coli used for detecting the heavy metal copper and establishment of a method for detecting copper in a water body by using the engineering strain of Escherichia coli. The engineering strain provided by the invention has the technical characteristics of specificity, sensitivity, rapidness, cheap price, stability and capacity of quantitative determination of the concentration and bio-availability of copper in a water body. The engineering strain has a wide detection linear range and high sensitivity in detection of copper ions, wherein the detection linear range is 0.39 to 94 [mu]M and lowest detection limit is 0.25 [mu]M; the engineering strain is applicable to both quantitative determination of copper in water bodies like various sewage, domestic drinking water, acidic mine wastewater and electroplating wastewater and to assessment of biotoxicity of treated or restored copper-contaminated water bodies; so the engineering strain and physical and chemical analytical methods like atomic absorption spectrometry can realize complementary analysis of bio-availability and total amount of copper in water bodies, thereby providing bases for objective evaluation of the biotoxicity of the heavy metal copper.
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Description

technical field

[0001] The invention relates to a construction method for detecting heavy metal copper Escherichia coli engineering strains and a specific implementation scheme for detecting heavy metal copper in water, belonging to the field of environmental biotechnology. Background technique

[0002] Copper (Cu), as one of the most common heavy metal elements, is an essential trace element for the metabolism of organisms. Studies have found that excessive copper or copper ions are toxic to all living organisms. Excessive deposition of copper in the human body is involved in the occurrence and development of many diseases. Copper can accumulate in vital organs of the human body, such as liver, kidney, brain, etc. Especially certain people with chromosomal recessive disorders. Due to the dysfunction of bile excretion of copper, copper accumulates in the liver, causing liver damage, chronic and active hepatitis symptoms, such as cirrhosis in Indian children, chronic interst...

Claims

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