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Preparation method of thermosensitive collagen-based composite hemostatic gel

A hemostatic gel and temperature-sensitive technology, which is applied in the field of temperature-sensitive collagen-based composite hemostatic gel and its preparation, can solve the problems of mechanical strength, gel strength to be improved, inability to quickly become gel, collagen bioactivity influence, etc. problems, to achieve the effect of short preparation process cycle, short gel time, and improved gel performance

Active Publication Date: 2017-05-31
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] (1) The viscosity is poor, and most of them need to be fixed with the outer dressing, and the mechanical strength and gel strength need to be improved;
[0006] (2) Collagen itself has certain gel properties, but it is far from being able to quickly become a gel under normal conditions. Combined agents, many will affect the biological activity of collagen;
[0007] (3) Collagen hydrogels are prepared by conventional repeated freezing and thawing methods, the preparation cycle is long, and the material is brittle and hard, which greatly limits the application of the material

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031](1) Preparation of Hydroxylated Regenerated Collagen Fiber Solution: Take 1 weight part of type I collagen and dissolve it in 100 volume parts of 10mM hydrochloric acid solution with stirring at 4°C. After the collagen is completely dissolved, slowly add the same amount of hydrochloric acid solution. A volume of Tris-NaCl buffer solution containing 30mM Tris and 0.1M NaCl with a pH value of 8.0 was stirred thoroughly at 4°C to obtain a buffer stock solution for regenerated collagen fibers; then the above-mentioned regeneration buffer stock solution was placed at a constant temperature of 25°C Incubate in an incubator for 5 hours to prepare a regenerated collagen fiber solution; finally, add transglutaminase at a concentration of 50 U / g to the above regenerated collagen fiber solution, and fully stir at 4°C for 10 hours to prepare a hydroxylated regenerated collagen fiber solution;

[0032] (2) Preparation of thermosensitive collagen-based composite hemostatic gel: 0.5 par...

Embodiment 2

[0034] (1) Preparation of Hydroxylated Regenerated Collagen Fiber Solution: Take 1.2 parts by weight of type I collagen and dissolve it in 150 parts by volume of 10mM hydrochloric acid solution with stirring at 5°C. A volume of Tris-NaCl buffer solution containing 40mM Tris and 0.13M NaCl with a pH value of 8.0 was stirred thoroughly at 5°C to obtain a regenerated collagen fiber buffer stock solution; then the above-mentioned regeneration buffer stock solution was placed at a constant temperature of 28°C Incubate in an incubator for 90 minutes to prepare a regenerated collagen fiber solution; finally, add transglutaminase at a concentration of 100 U / g to the above regenerated collagen fiber solution, and stir fully at 5°C for 15 hours to prepare a hydroxylated regenerated collagen fiber solution;

[0035] (2) Preparation of thermosensitive collagen-based composite hemostatic gel: add 1 part by weight of poloxamer 407 and 1 part by weight of polyvinylpyrrolidone to the above hyd...

Embodiment 3

[0037] (1) Preparation of Hydroxylated Regenerated Collagen Fiber Solution: Take 5 parts by weight of type I collagen and dissolve it in 500 parts by volume of 10mM hydrochloric acid solution with stirring at 4-10°C. After the collagen is completely dissolved, slowly add hydrochloric acid and The same volume of Tris-NaCl buffer solution containing 50mM Tris and 0.2M NaCl with a pH value of 8.0 was stirred thoroughly at 10°C to obtain a regenerated collagen fiber buffer stock solution; then the above-mentioned regeneration buffer stock solution was placed at 37°C In a constant temperature incubator, incubate for 0.5h to prepare a regenerated collagen fiber solution; finally, add transglutaminase with a concentration of 150U / g to the above regenerated collagen fiber solution, stir well at 10°C for 24h, and prepare a hydroxylated regenerated collagen fiber solution;

[0038] (2) Preparation of temperature-sensitive collagen-based composite hemostatic gel: 5 parts by weight of pol...

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Abstract

The present invention discloses a thermosensitive collagen-based composite hemostat gel and a preparation method thereof. The method is characterized by comprising the steps of preparing a regeneration collagen fiber solution by utilizing collagen type I in a neutral buffer environment in a constant temperature incubator at 25-37 DEG C through the molecular self-assembly process firstly; subjecting the regeneration collagen fiber solution to hydroxylation modification by utilizing glutamine transaminase to obtain a hydroxylated regeneration collagen fiber solution; adding poloxamer 407, polyvinylpyrrolidone and a hemostatic drug in the above hydroxylated regeneration collagen fiber solution and fully stirring the obtained mixture to finally prepare the thermosensitive collagen-based composite hemostat gel. The thermosensitive collagen-based composite hemostat gel is excellent in physiological hemostasis activity, temperature sensitive property, biodegradable property, biocompatibility, adhesivity and the like. By means of the gel, the bleeding is stopped quickly, and the antibacterial effect is realized. Meanwhile, wounds are kept moist, and the healing and repairing process of wounds is facilitated. Therefore, the thermosensitive collagen-based composite hemostat gel can be widely used for the hemostatic repair of wounds.

Description

technical field [0001] The invention relates to a temperature-sensitive collagen-based composite hemostatic gel and a preparation method thereof, belonging to the field of preparation of biomedical materials. Background technique [0002] Extensive bleeding and oozing in surgical and traumatic operations pose great dangers to human life. Physiological hemostasis alone is far from achieving rapid and effective hemostasis. Therefore, it is necessary to introduce exogenous substances to accelerate wound hemostasis. Hemostatic materials have always been a research hotspot in the field of biomaterials, especially absorbable hemostatic materials. At present, the main absorbable hemostatic materials at home and abroad include fibrin glue, gelatin, collagen, oxidized cellulose, chitosan and calcium alginate, etc. These marketed products are mostly in the form of sponge, fiber, powder, film and other dosage forms. Although the hemostatic effect of these products can meet the needs of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/42A61L15/32A61L15/24A61L15/26A61L15/44
Inventor 但卫华刘新华但年华龚居霞
Owner SICHUAN UNIV
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