Composition with anti-aging function and preparation thereof

A composition and anti-aging technology, which is applied in the field of medical cosmetology, can solve the problems of inconspicuous promoting effect and limited anti-aging effect, and achieve good anti-aging effect, rich nutritional ingredients, and excellent anti-aging effect

Active Publication Date: 2015-09-23
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the anti-aging effect that can be achieved by relying solely on stem cell growth factor is limited,

Method used

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  • Composition with anti-aging function and preparation thereof
  • Composition with anti-aging function and preparation thereof
  • Composition with anti-aging function and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Preparation of Stem Cell Growth Factor

[0063]Obtain primary mesenchymal stem cells from autologous adipose tissue by enzymatic digestion. The process is as follows: adipose tissue solution obtained by liposuction, rinsed with PBS (phosphate buffered saline) for 2 to 3 times, and added BSA with a final concentration of 1 wt%. (bovine serum albumin) and collagenase type 1 with a final concentration of 0.1wt%, shake in a shaking incubator at 37°C for 30min; then add X-VIVO15 containing 10% (volume percent concentration) FBS (fetal bovine serum) Serum-free medium was used to stop the digestion; after that, the undigested tissue was filtered through an 80-mesh mesh, and the filtrate was centrifuged at 400 g for 10 min; finally, the upper three layers were discarded, and the adipose stem cells in the lower layer were the primary mesenchymal stem cells isolated in this example.

[0064] Primary mesenchymal stem cells were inoculated in LONZA X-VIVO15 serum-free medium contai...

Embodiment 2

[0067] Preparation of honeysuckle extract

[0068] First take honeysuckle powder, add 10 times the mass of 50wt% ethanol, extract twice at 80°C for two hours each time, combine the immersion solution and filter; then the filtrate is concentrated under reduced pressure, ethanol is recovered, and the pH of the concentrated solution is adjusted to about 2 , extracted four times with 4 times the amount of ethyl acetate, combined the extracts; then added an appropriate amount of water to the extracts to wash twice, adjusted the pH to 6-7 with 40wt% NaOH, allowed the layers to stand, and took the water layer; Finally, the water layer is pressurized and concentrated to a thick paste, and air-dried at room temperature to obtain the honeysuckle extract.

[0069] The composition of the above-mentioned honeysuckle extract was analyzed, and the results were as follows: the content of neochlorogenic acid was 4 wt%, the content of chlorogenic acid was 10 wt%, the content of caffeic acid was...

Embodiment 3

[0071] Preparation of Biologics

[0072] Formulation design:

[0073]

[0074] Formulation preparation:

[0075] Prepare a hyaluronic acid aqueous solution containing stem cell growth factor freeze-dried powder, wherein the content of stem cell growth factor freeze-dried powder is 1 mg / mL, and the content of hyaluronic acid is 500 ng / mL; prepare honey aqueous solution with a honey content of 1 g / mL; prepare honeysuckle extract Aqueous solution of honeysuckle extract with a content of 0.01g / mL. Mix the above three solutions evenly, filter through a 0.22 μm filter membrane, and then concentrate under reduced pressure and vacuum until the content of each component reaches the content designed in the formula to obtain a biological preparation with anti-aging effect.

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Abstract

The invention belongs to the field of medical cosmetology, and particularly relates to composition with an anti-aging function and preparation thereof. The composition comprises, by weight, 0.03-4 parts of stem cell growth factors, 1-30 parts of honeysuckle extracts, 50-300 parts of honey and 0.1*10<-3>-1*10<-3> parts of hyaluronic acid. The stem cell growth factors comprise at least two of epidermal growth factors, fiber cell growth factors, platelet-derived growth factors and vascular endothelial growth factors. According to the composition with the anti-aging function and the preparation, multiple stem cell growth factors are added into the composition, the multiplication capacity of epidermis cells is improved remarkably, meanwhile the composition formula is optimized, and the effects of all components in the composition are played effectively, so that the composition has rich nutritional ingredients, the good moisturizing effect and the antioxidant effect, and the composition presents excellent anti-aging effect.

Description

technical field [0001] The invention belongs to the field of medical cosmetology, and in particular relates to a composition with anti-aging effect and its preparation. Background technique [0002] Skin is an important organ covering the surface of the human body. It is composed of epidermis, dermis, subcutaneous tissue and skin appendages. It is a natural barrier between the external environment and the body. However, the protective effect of the skin is limited, and the human skin enters a natural aging state from the age of 20 to 25. On the one hand, with the increase of age, the amount of sebum secretion decreases, and the interstitium of keratinocytes also decreases, so the water retention capacity decreases, the water barrier function of the skin gradually declines, and dry skin begins to appear; on the other hand, epidermal cells The differentiation ability is reduced, the recovery rate of the epidermis is slowed down, the keratinization function is reduced, the str...

Claims

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Application Information

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IPC IPC(8): A61K38/18A61P17/18A61K36/355A61K35/644A61K31/728
Inventor 陈海佳王一飞葛啸虎应杰万桦
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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