Composition with anti-aging function and preparation thereof
A composition and anti-aging technology, which is applied in the field of medical cosmetology, can solve the problems of inconspicuous promoting effect and limited anti-aging effect, and achieve good anti-aging effect, rich nutritional ingredients, and excellent anti-aging effect
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Embodiment 1
[0062] Preparation of Stem Cell Growth Factor
[0063]Obtain primary mesenchymal stem cells from autologous adipose tissue by enzymatic digestion. The process is as follows: adipose tissue solution obtained by liposuction, rinsed with PBS (phosphate buffered saline) for 2 to 3 times, and added BSA with a final concentration of 1 wt%. (bovine serum albumin) and collagenase type 1 with a final concentration of 0.1wt%, shake in a shaking incubator at 37°C for 30min; then add X-VIVO15 containing 10% (volume percent concentration) FBS (fetal bovine serum) Serum-free medium was used to stop the digestion; after that, the undigested tissue was filtered through an 80-mesh mesh, and the filtrate was centrifuged at 400 g for 10 min; finally, the upper three layers were discarded, and the adipose stem cells in the lower layer were the primary mesenchymal stem cells isolated in this example.
[0064] Primary mesenchymal stem cells were inoculated in LONZA X-VIVO15 serum-free medium contai...
Embodiment 2
[0067] Preparation of honeysuckle extract
[0068] First take honeysuckle powder, add 10 times the mass of 50wt% ethanol, extract twice at 80°C for two hours each time, combine the immersion solution and filter; then the filtrate is concentrated under reduced pressure, ethanol is recovered, and the pH of the concentrated solution is adjusted to about 2 , extracted four times with 4 times the amount of ethyl acetate, combined the extracts; then added an appropriate amount of water to the extracts to wash twice, adjusted the pH to 6-7 with 40wt% NaOH, allowed the layers to stand, and took the water layer; Finally, the water layer is pressurized and concentrated to a thick paste, and air-dried at room temperature to obtain the honeysuckle extract.
[0069] The composition of the above-mentioned honeysuckle extract was analyzed, and the results were as follows: the content of neochlorogenic acid was 4 wt%, the content of chlorogenic acid was 10 wt%, the content of caffeic acid was...
Embodiment 3
[0071] Preparation of Biologics
[0072] Formulation design:
[0073]
[0074] Formulation preparation:
[0075] Prepare a hyaluronic acid aqueous solution containing stem cell growth factor freeze-dried powder, wherein the content of stem cell growth factor freeze-dried powder is 1 mg / mL, and the content of hyaluronic acid is 500 ng / mL; prepare honey aqueous solution with a honey content of 1 g / mL; prepare honeysuckle extract Aqueous solution of honeysuckle extract with a content of 0.01g / mL. Mix the above three solutions evenly, filter through a 0.22 μm filter membrane, and then concentrate under reduced pressure and vacuum until the content of each component reaches the content designed in the formula to obtain a biological preparation with anti-aging effect.
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