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Growth regulator capable of regulating growth and other functions of microalgae, and verification method and application thereof

A growth regulator and microalgae technology, which is applied in the field of biological metabolism engineering, can solve the problems of not being able to effectively increase oil accumulation, and the level of oil accumulation in algae has not been significantly improved, and achieve the effect of improving economic traits, improving physiological traits, and high-efficiency expression

Inactive Publication Date: 2015-09-30
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 1990, acetyl-CoA carboxylase (acetyl-CoA carboxylase, ACC) was first cloned in Cyclotella cryptica (Roessler, 1990), and was subsequently successfully used in the transformation of diatoms C. cryptica and Navicula saprophila; Detection of ACC enzyme activity in engineered microalgae increased 2-3 times, but the accumulation level of oil in algae did not significantly increase (Dunahay et al., 1996)
This shows that the modification of a single enzyme in the lipid metabolism pathway cannot effectively improve the accumulation of lipids

Method used

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  • Growth regulator capable of regulating growth and other functions of microalgae, and verification method and application thereof
  • Growth regulator capable of regulating growth and other functions of microalgae, and verification method and application thereof
  • Growth regulator capable of regulating growth and other functions of microalgae, and verification method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: Identification of algae hormones

[0046] Step 1. Algae species selection The algae species involved in the present invention include (1) green algae, such as Micromonas sp.RCC299, Ostreococcus tauri, Chlorella variabilis NC64A, Haematococcus pluvialis, Chlorella pyrenoidosa, Chlorella subellipsoidea C-169, Chlamydomonas reinhardtii, Volvox carteri, etc. (2) Red algae, such as Cyanidioschyzon merolae, Porphyridium purpureum, Gelidium amansii, Gloiopeltis furcata, Caloglossa leprieurii, Digenea simples, etc.; (3) Diatoms, such as Phaeodactylum tricornutum, Thalassiosira pseudonana, Fragilariopsis cylindrus, etc.; (4) Brown algae, such as Pelvetia canaliculata, Ecklonia hornem, Undaria pinnatifida, Ectocarpus siliculosus, etc.; (5) Pelvetia canaliculata, such as Nannochloropsis oceanica, etc., but not limited to the above algae species. The microalgae can be obtained commercially or screened from nature.

[0047] Step 2. Algae cultivation

[0048] The above-m...

Embodiment 2

[0059] Example 2: Identification of key enzymes or proteins in algae hormone metabolism and regulation

[0060]Step 1. Algae species selection The algae species involved in the present invention include (1) green algae, such as Micromonas sp.RCC299, Ostreococcus tauri, Chlorella variabilis NC64A, Haematococcus pluvialis, Chlorella pyrenoidosa, Chlorella subellipsoidea C-169, Chlamydomonas reinhardtii, Volvox carteri, etc. (2) Red algae, such as Cyanidioschyzon merolae, Porphyridium purpureum, Gelidium amansii, Gloiopeltis furcata, Caloglossa leprieurii, Digenea simples, etc.; (3) Diatoms, such as Phaeodactylum tricornutum, Thalassiosira pseudonana, Fragilariopsis cylindrus, etc.; (4) Brown algae, such as Pelvetia canaliculata, Ecklonia hornem, Undaria pinnatifida, Ectocarpus siliculosus, etc.; (5) Pelvetia canaliculata, such as Nannochloropsis oceanica, etc., but not limited to the above algae species. The microalgae can be obtained commercially or screened from nature.

[00...

Embodiment 3

[0069] Example 3: Metabolic response of microalgae hormones under nitrogen deficiency stress

[0070] Step 1. Algae species selection The algae species involved in the present invention include (1) green algae, such as Micromonas sp.RCC299, Ostreococcus tauri, Chlorella variabilis NC64A, Haematococcus pluvialis, Chlorella pyrenoidosa, Chlorella subellipsoidea C-169, Chlamydomonas reinhardtii, Volvox carteri, etc. (2) Red algae, such as Cyanidioschyzon merolae, Porphyridium purpureum, Gelidium amansii, Gloiopeltis furcata, Caloglossa leprieurii, Digenea simples, etc.; (3) Diatoms, such as Phaeodactylum tricornutum, Thalassiosira pseudonana, Fragilariopsis cylindrus, etc.; (4) Brown algae, such as Pelvetia canaliculata, Ecklonia hornem, Undaria pinnatifida, Ectocarpus siliculosus, etc.; (5) Pelvetia canaliculata, such as Nannochloropsis oceanica, etc., but not limited to the above algae species. The microalgae can be obtained commercially or screened from nature.

[0071] Step ...

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Abstract

The invention relates to the technical field of biological metabolism engineering, particularly a metabolism and signal pathway of a microalga growth regulator, and a verification method and application thereof. A metabolomic means is utilized to verify microalga hormones, and a functional genomic means is utilized to explain the metabolism channel, signal transmission pathway and regulation network of the microalga hormones; a transcriptomic means is utilized to verify key enzymes and proteins related to hormone metabolism and signal transmission in the microalga stress tolerance and oil synthesis process; and metabolism engineering and chemical engineering means are utilized to regulate the hormone metabolism and signal pathway, thereby optimizing the physiological characters of the algae and plants. The invention provides a method for verifying microalga hormones and metabolism pathway / signal pathway thereof. The method can explain the hormone metabolism enzymes and signal proteins closely related to physiology of algae as well as related regulation mechanism, and is used for improving the agronomic characters of organisms.

Description

technical field [0001] The invention relates to the technical field of biological metabolism engineering, in particular to a growth regulator capable of regulating the growth of microalgae and other physiological functions, its identification method and application. Background technique [0002] Microalgae are considered to be one of the most potential oily biomass resources. Although the advantages of microalgae for biofuel production are obvious, its economic feasibility is still facing great challenges. The main technical bottlenecks include low growth density of microalgae, poor stress resistance and low oil content. On the other hand, with the development of microalgae biotechnology, genetic engineering technology and molecular biology technology, the isolation, cloning and regulation of key genes in lipid metabolism pathways have gradually become possible. In 1990, acetyl-CoA carboxylase (acetyl-CoA carboxylase, ACC) was first cloned in Cyclotella cryptica (Roessler, ...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12Q1/68C12R1/89
CPCC12N1/12C12Q1/6895
Inventor 路延笃徐健
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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