Monodisperse Magnetic Silica Composite Microspheres and Its Preparation Process

A technology of silica and composite microspheres, which is applied in the field of magnetic materials, can solve the problems of difficult control of particle size and varying amounts of nanoparticles, and achieve the effects of controllable particle size, high product purity and simple operation

Active Publication Date: 2017-08-11
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There are defects in the current process. The above method of synthesizing magnetic silica composite materials based on the Stober method is difficult to control the particle size, and the core contains Fe 3 o 4 The amount of nanoparticles is different

Method used

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  • Monodisperse Magnetic Silica Composite Microspheres and Its Preparation Process
  • Monodisperse Magnetic Silica Composite Microspheres and Its Preparation Process

Examples

Experimental program
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Effect test

Embodiment 1

[0028] a) Processing freshly collected cyanobacteria

[0029] 1. Centrifugal concentration: Collect fresh cyanobacteria by centrifugation. Centrifuge speed 2500 rpm, centrifuge 10min;

[0030] 2. Aldehyde fixation: fix cyanobacteria with 4% formaldehyde solution;

[0031] 3. Cleaning: Wash the cyanobacteria fixed with formaldehyde solution with double distilled water, wash 3 times, each time for 5 minutes, to remove the residual formaldehyde on the surface;

[0032] 4. Dehydration: Collect the washed cyanobacteria by centrifugation at a speed of 2500 rpm for 10 minutes. Use 30%~100% alcohol solution to dehydrate blue-green algae in gradient, each time for 20 minutes; use 100% alcohol to dehydrate twice, each time for 20 minutes;

[0033] 2) Preparation of monodisperse magnetic silica composite microspheres using treated cyanobacteria as templates

[0034] Add 0.046mol Fe to 50mL of double distilled water that has passed nitrogen 3+ and 0.023mol Fe 2+ , to obtain solution...

Embodiment 2

[0040] The difference between this embodiment and the specific example 1 is: the process of preparing monodisperse magnetic silica composite microspheres using the treated cyanobacteria as a template, and other steps are the same as in the first example;

[0041] Add 0.046mol Fe 3+ and 0.023mol Fe 2+, to obtain solution I; mix absolute ethanol, double distilled water, and ammonia water in a volume ratio of 3:3:1 to obtain solution II; mix ethyl orthosilicate and absolute alcohol in a volume ratio of 2:3 to obtain Solution III. The preparation process is carried out in three steps:

[0042] Step 1: Add the treated cyanobacteria into solution I, stir at a low speed, and infiltrate at 25°C for 30 hours. After infiltration, wash the cyanobacteria three times with double distilled water to remove the solvent on the surface of the cyanobacteria;

[0043] The second step: Put the cyanobacteria treated in the first step into 23mL solution II, stir at a low speed, infiltrate at 25°...

Embodiment 3

[0047] The difference between this embodiment and the specific example 1 is: the process of preparing monodisperse magnetic silica composite microspheres using the treated cyanobacteria as a template, and other steps are the same as in the first example;

[0048] Add 0.046mol Fe to 100mL of double distilled water that has passed nitrogen 3+ and 0.023mol Fe 2+ , to obtain solution I; mix absolute ethanol, double distilled water, and ammonia water in a volume ratio of 5:5:2 to obtain solution II; mix ethyl orthosilicate and absolute alcohol in a volume ratio of 4:5 to obtain Solution III. The preparation process is carried out in three steps:

[0049] Step 1: Add the treated cyanobacteria into solution I, stir at a low speed, and infiltrate at 25°C for 45 hours. After infiltration, wash the cyanobacteria three times with double distilled water to remove the solvent on the surface of the cyanobacteria;

[0050] The second step: Put the cyanobacteria treated in the first step ...

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Abstract

The invention relates to a monodispersed magnetic silicon dioxide composite microsphere prepared by taking blue-green algae as a template and a preparation method thereof. The technological process comprises the steps of carrying out segmented permeation and then carrying out high temperature treatment on treated fresh blue-green algae to obtain a target product. The specific steps are as follows: step one, stirring and permeating blue-green algae in a mixing solution containing Fe<2+> or Co<2+> or Mn<2+> and Fe<3+> or Co<3+> salt, and then washing for three times by using double distilled water; step two, adding centrifuged permeating blue-green algae to an alkaline alcohol-water solution, stirring for permeation and washing; and step three, centrifuging washed blue-green algae and then adding centrifuged blue-green algae to an alcoholic solution of ortho-silicate ester, stirring for permeation and centrifuging and conducting high temperature treatment on a centrifuged product to remove a blue-green algae template, so as to obtain a monodispersed magnetic silicon dioxide composite microsphere with grain size of 1-1.5 micrometers. The composite microsphere and the preparation method thereof have the advantages of being simple in operation, easy in process control, environment-friendly and low in cost; the product purify is high, the grain size is uniform, the magnetic response speed is high, the shape is neat, no hard agglomeration is generated, and thus the composite microsphere is suitable for industrial production. The composite microsphere and the preparation method thereof have a wide application prospect in the aspects of cell separation, enzyme immobilization, protein separation and the like.

Description

technical field [0001] The invention belongs to the technical field of magnetic materials, and in particular relates to a monodisperse magnetic silicon dioxide composite microsphere and a preparation process thereof. Background technique [0002] Cyanobacteria mainly grow in fresh water and are important phytoplankton in fresh water. They often multiply in large numbers in warm seasons to form "algae blooms", causing serious environmental pollution. Therefore, the comprehensive utilization of cyanobacteria has huge economic and social benefits. [0003] Due to its good chemical stability and biocompatibility, magnetic silica particles have a very wide range of applications in the fields of biotechnology and medicine, such as the separation and purification of cells, proteins, DNA and RNA, and the directional delivery of drugs. In practical applications, microspheres are generally required to have the characteristics of monodispersity, controllable particle size, and fast mag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J13/02
Inventor 任天瑞张博张雷于广金杨姗王全喜任帅臻
Owner SHANGHAI NORMAL UNIVERSITY
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