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Application of a kind of total flavonoids in leaves

A technology of total flavonoids and structuring leaves, applied in the application field of plant extracts, can solve the problems of no inhibition of cerebral ischemia and myocardial ischemia

Active Publication Date: 2020-03-20
HEBEI YILING MEDICINE INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A plurality of flavonoids have been isolated from the whole plant of the mulberry tree, and the effects of the ethanol extract (BPAE) and total flavonoid glycosides (BPF) of the mulberry leaf on the isolated atrium of rabbits and guinea pigs have been studied, and BPAE has been found Both BPF and BPF have the effect of inhibiting the atrial contraction of rabbits and guinea pigs, and the potency of BPF is much greater than that of BPAE, which proves that BPF is the main active ingredient of mulberry tree in inhibiting atrial contraction, and also shows that the leaves of mulberry tree have a similar calcium antagonist effect. The total flavonoids of the leaves of S. arbusae also have the effect of inhibiting human liver cancer cells, but there is no report on the inhibition of cerebral ischemia and myocardial ischemia.

Method used

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  • Application of a kind of total flavonoids in leaves
  • Application of a kind of total flavonoids in leaves
  • Application of a kind of total flavonoids in leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0107] : Embodiment 1: the preparation of the total flavonoids of leaves

[0108] A. Weigh 2 kg of the leaves of the tree, add 12 times the amount of 60% ethanol to soak for 30 minutes, reflux extraction 3 times, each time for 2 hours, filter, and concentrate the filtrate under reduced pressure until the crude drug concentration is 1g / ml, add 5 times the amount of water, mix Mix well, refrigerate at 4-10°C for 24 hours, filter, and use the filtrate for later use;

[0109] B. Macroporous adsorption resin purification: the filtrate obtained in step A is adjusted to ph=3-4 with hydrochloric acid, and purified with macroporous adsorption resin. Double column volume per hour, saturated for 1 hour, eluted with water for 6 times of column volume, 40% ethanol for 6 times of column volume, recovered with 40% ethanol eluent, concentrated under reduced pressure to a concentrated solution with a concentration of 0.2 g / ml, spare;

[0110] C. Polyamide purification: the concentrated solut...

Embodiment 2

[0111] Embodiment 2: Determination method of total flavonoids content in leaves

[0112]A. Preparation of the test solution: Take 0.2g of the total flavonoids powder of the leaves, add 150ml of 60% ethanol, seal, reflux and extract for 60 minutes, filter the extract, dilute to 200ml with 60% ethanol, accurately draw 1ml, add distilled water 5ml, then add 1ml of 5% sodium nitrite solution, and let it stand for 6 minutes; then add 1ml of 10% aluminum nitrate, let it stand for 6 minutes; , make a negative control solution according to the above steps;

[0113] B. Preparation of the standard curve: take 10 mg of the reference substance of total flavonoids from the leaves, accurately weigh it, place it in a 25ml volumetric flask, add 60% ethanol to dissolve and dilute to the mark, shake well, and obtain the first-grade stock solution of the reference substance, which is accurately drawn respectively 1.0, 2.0, 3.0, 4.0, 5.0ml of the primary stock solution of the reference substance...

Embodiment 3

[0115] Embodiment 3: tablet preparation

[0116] Weigh 100g of the total flavonoids of the leaves of the tree, add 100g of microcrystalline cellulose, 200g of lactose, mix well, add croscarmellose sodium, mix evenly, granulate with a 24 mesh sieve, dry at 70°C, and granulate. Add 1% magnesium stearate, mix well, and compress to make 1000 tablets.

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Abstract

The invention discloses the application of the total flavonoids of the leaves of sarcocarpus, which belongs to the field of medicine application. The medicine has obvious inhibitory effect on cerebral ischemia or myocardial ischemia, and can be taken for a long time without side effects.

Description

technical field [0001] The invention belongs to the field of medicine application, and in particular relates to the application of plant extracts. Background technique [0002] The paper mulberry is a Moraceae plant, and the paper mulberry is distributed in the temperate zone and the tropics of China. It can grow regardless of plains or hills. This tree species has fast growth, strong adaptability, wide distribution, easy reproduction, and heat. The leaves of the mulberry tree have the functions of clearing away heat, cooling blood, promoting dampness, killing insects, and are used for epistaxis, enteritis, and dysentery. [0003] Since the 1980s, people have begun to study the chemistry and pharmacology of Sorrel tree, see Gao Yunsheng, Qiu Yufang, Gao Ling, etc. Inhibitory effect of syringa alcohol extract and flavonoid glycosides on isolated atrium[J].China Pharmacological Bulletin, 1988, 4(2): 122-123. A plurality of flavonoids have been isolated from the whole plant o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/60A61P9/10A61K127/00
Inventor 姜新刚赵韶华贾继明宋剑刘静媛高贤苏平菊柴振平姬雪礼李文烈
Owner HEBEI YILING MEDICINE INST