Method for comprehensively extracting fucoxanthin and fucoidan from sargassum

A technology of fucoxanthin and fucoidan, applied in the direction of organic chemistry and the like, can solve the problems such as no disclosure of fucoxanthin and fucoidan sulfate, inability to realize technology popularization and large-scale production, low extraction efficiency and low utilization rate of raw materials, To achieve the effect of simple and easy to obtain equipment, low cost and low cost

Active Publication Date: 2015-11-11
NINGBO UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The extraction methods in the above patents are all methods for extracting fucoxanthin or polysaccharide sulfate alone, and the extraction efficiency and raw material utilization rate are low; at the same time, the production cost is generally high, and t

Method used

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  • Method for comprehensively extracting fucoxanthin and fucoidan from sargassum

Examples

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Effect test

Embodiment 1

[0041] Wash the fresh copper algae 3 times with water to remove surface impurities and salt, then blot the surface moisture with absorbent cotton, control the water content of the copper algae to 70%, cut it with scissors, weigh 200g copper algae, put it into a 1000mL Erlenmeyer flask, Add 600mL of 80% (V / V) ethanol aqueous solution, wrap it with tinfoil paper, put it in a shaker and extract in the dark for 4 hours, filter to obtain the filtrate and filter residue; the filtrate (A) is concentrated in vacuum at 40°C until the solution appears Black precipitate, filter; continue to concentrate until a red solid precipitates out of the solution, and the obtained red solid is high-purity fucoxanthin with a purity of 85%; The ethanol aqueous solution with a volume fraction of 80% was refluxed for 3 hours; water was added to the extracted solid, the ratio of solid to liquid was 1:5, and extracted in a water bath at 70°C for 5 hours; Centrifuge in a centrifuge under the condition of ...

Embodiment 2

[0043]Wash fresh hijiki twice with clean water to remove surface impurities and salt, then blot the surface moisture with absorbent cotton, control the moisture content of hijiki to 50%, cut it into pieces with scissors, weigh 200g hijiki, put it into a 1000mL cone Shaped bottle, add 600mL of 80% (V / V) ethanol aqueous solution, wrap it with tin foil, put it in a shaker and extract it in the dark for 1 hour, filter to obtain the filtrate and filter residue; the filtrate (A) is concentrated in vacuum at 35°C until Black precipitate appears in the solution, filter; continue to concentrate until a red solid is precipitated in the solution, and the obtained red solid is high-purity fucoxanthin with a purity of 85%; the filter residue (B) is put into a Soxhlet extractor, 60 ℃, reflux with ethanol aqueous solution with a volume fraction of 60% for 5 hours; add water to the extracted solids, the ratio of solid to liquid is 1:2, and extract in a water bath at 60 ℃ for 8 hours; filter th...

Embodiment 3

[0045] Wash fresh sea sorghum 5 times with clean water to remove surface impurities and salt, then use absorbent cotton to blot the surface moisture, control the water content of sea sorghum to 90%, cut it with scissors, weigh 200g of sea sorghum, put it into a 1000mL cone Shaped bottle, add 600mL of 80% (V / V) ethanol aqueous solution, wrap it with tin foil paper, put it in a shaker and extract it in the dark for 8 hours, filter to obtain the filtrate and filter residue; the filtrate (A) is concentrated in vacuum at 45°C until Black precipitate appears in the solution, filter; continue to concentrate until a red solid is precipitated in the solution, and the obtained red solid is high-purity fucoxanthin with a purity of 85%; the filter residue (B) is put into a Soxhlet extractor, 90 ℃, reflux with ethanol aqueous solution with a volume fraction of 99% for 1 hour; add water to the extracted solids, and extract in a 90 ℃ water bath for 3 hours; The condition is 4000r, 15min; tak...

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Abstract

The invention discloses a method for comprehensively extracting fucoxanthin and fucoidan from sargassum. The method for comprehensively extracting fucoxanthin and fucoidan from the sargassum is characterized by comprising the following steps: unfreezing fresh sargassum or frozen sargassum at room temperature, washing with clear water, and controlling moisture content of the sargassum to be 50-90%; adding the sargassum into ethanol water, shaking up, wrapping a conical flask with tinfoil, putting the conical flask into a shaking table, extracting for 1-8 hours at normal temperature, and filtering to obtain filtrate; carrying out vacuum concentration on the filtrate until a large amount of black substance is separated out, and continuously carrying out vacuum concentration until red solid, namely high-purity fucoxanthin, is separated out; putting filter residue into a Soxhlet extractor, refluxing with ethanol water, extracting for 1-5 hours at the temperature of 60-90 DEG C, soaking and extracting the extracted solid matter in water for 3-8 hours at the temperature of 60-90 DEG C, filtering, centrifuging, taking supernate, carrying out vacuum concentration, adding ethanol, uniformly mixing, filtering, taking filter residue, washing with ethanol, and drying, so that the fucoidan is obtained. The method for comprehensively extracting the fucoxanthin and fucoidan from the sargassum has the advantages that raw material utilization rate is high, the extraction efficiency is high, and the method is simple and feasible.

Description

technical field [0001] The invention relates to a method for comprehensively extracting fucoxanthin and fucoidan sulfate, in particular to a method for comprehensively extracting fucoxanthin and fucoidan sulfate from sargassum. Background technique [0002] Fucoxanthin, also known as fucoxanthin, is a carotenoid compound, which has a strong antioxidant effect because of its special allene carbon skeleton in its structure. Modern pharmacological studies have shown that fucoxanthin has various activities such as anti-tumor, weight loss, anti-inflammation, and anti-oxidation, and has great medicinal value. At present, fucoxanthin cannot be produced on a large scale through chemical synthesis. Therefore, extracting fucoxanthin from seaweed has become a requirement of modern society. [0003] Fucoidan, also known as fucoidan, fucoidan sulfate, fucoidan, etc., is a polysaccharide active substance present in the cell wall of brown algae. The chemical structure of fucoidan sulfate...

Claims

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Application Information

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IPC IPC(8): C08B37/04C07D303/32
Inventor 张金荣周成旭严小军何山陈海敏骆其君朱鹏段海霞
Owner NINGBO UNIV
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