Phospholipid classification detection and quantification method based on stable isotope labeling

A stable isotope and isotope labeling technology, which is applied in the direction of measuring devices, material separation, and analysis of materials, can solve the problems of limited types, storage restrictions, and high prices, and achieve the effects of sensitive identification, quantitative comparative analysis, and enhanced sensitivity
CN105067697AActive Publication Date: 2015-11-18INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
Publication Date
2015-11-18

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Abstract

The present invention discloses a phospholipid classification detection and quantification method based on stable isotope labeling, and belongs to the technical field of phospholipid quantification detection methods. According to the method, mainly trimethylsilyl diazomethane is used to respectively generate diazomethane and deuterium-labeled diazomethane in a methyl tert-butyl ether / methanol / 1N hydrochloric acid solution system and a methyl tert-butyl ether / deuteromethanol / 1N deuterium chloride solution system in an in-situ manner so as to further respectively carry out methyl esterification on the phosphoric acid group or carboxylic acid group in the phospholipid molecule to generate the light isotope labeled-phospholipid methyl esterification derivative and the heavy isotope labeled-phospholipid methyl esterification derivative, wherein the light isotope labeled-phospholipid methyl esterification derivative and the heavy isotope labeled-phospholipid methyl esterification derivative have the same physical and chemical properties and different molecular weights; and the relative intensity of the light isotope labeled-mass spectrum peak signal and the heavy isotope labeled-mass spectrum peak signal are compared to associate with the phospholipid molecule amount in the sample so as to achieve the relative quantification on the phospholipid. The method of the present invention has characteristics of enhanced sensitivity and completion within a shor time.
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Description

technical field

[0001] The invention relates to the technical field of phospholipid quantitative detection methods, in particular to a stable isotope label-based phospholipid classification detection and quantitative method. Background technique

[0002] Phospholipids are not only an important component of cell membranes, but also widely involved in the regulation of various life processes, including energy conversion, material transport, information recognition and transmission, cell development and differentiation, and cell apoptosis. Abnormal metabolism of phospholipids is also closely related to arteriosclerosis, diabetes, obesity, Alzheimer's disease and tumorigenesis. Therefore, from the perspective of "omics", systematic and large-scale research on the dynamic changes in the composition and relative quantity of phospholipid molecules under different physiological or pathological conditions will be of great help in revealing the relationship between phospholipid metabo...

Claims

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