Preparation method and applications of magnetic nanocomposite particle

A composite particle, magnetic nanotechnology, applied in biochemical equipment and methods, chemical instruments and methods, alkali metal compounds, etc., can solve the problems of many steps, short production cycle, complex separation process, etc., to improve extraction efficiency, specificity, etc. The effect of good adsorption and simple operation

Inactive Publication Date: 2015-12-02
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using the prepared magnetic nano-composite particles to separate and purify lysozyme from egg white, the operation is simple, the cost is low, the production cycle is short, the separation efficiency is high, the separa

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0037] Example 1

[0038] 0.01M ferrous sulfate heptahydrate and 0.02M ferric chloride hexahydrate are dissolved in water-ethanol mixed solution (V / V=10:1), add polyethylene glycol 6000, under ultrasonic (F=28KHz) dispersion state Nitrogen gas for 30 minutes; raise the temperature to 40°C, then slowly add ammonia water to the system, adjust the pH value of the system to about 10, and obtain a black solution containing magnetic particles, after reacting for 30 minutes, quickly add 2% sodium chondroitin sulfate solution , heated up to 70°C for 1 hour, terminated the reaction, left to cool to obtain the reaction liquid; separated the magnetic particles under the action of an external magnetic field, washed several times with deionized water until neutral, and performed freeze-drying treatment to obtain Sodium chondroitin sulfate modified iron ferric oxide magnetic nanocomposite particle powder.

[0039] Egg yolk and egg white were separated, and then fresh egg white was dilute...

Example Embodiment

[0041] Example 2

[0042] Dissolve 0.2M ferrous chloride tetrahydrate and 0.1M ferric chloride hexahydrate in water-ethanol mixed solution (V / V=1:1), add sodium dodecylbenzenesulfonate, and ) in a dispersed state and pass nitrogen gas for 30 minutes; raise the temperature to 60°C, then slowly add sodium hydroxide solution to the system, adjust the pH value of the system to about 11, and obtain a black solution containing magnetic particles, add it quickly after reacting for 30 minutes 2% citric acid solution, heated up to 80°C for 1 hour, terminated the reaction, let it stand for cooling, and obtained the reaction solution; separated the magnetic particles under the action of an external magnetic field, washed several times with deionized water until neutral, and lyophilized After treatment, the citric acid-modified ferroferric oxide magnetic nanocomposite particle powder can be obtained.

[0043] Egg yolk and egg white were separated, and then fresh egg white was diluted w...

Example Embodiment

[0045] Example 3

[0046] Dissolve 0.2M ferrous sulfate heptahydrate and 0.3M ferric chloride hexahydrate in water-ethanol mixed solution (V / V=5:1), add polyethylene glycol 6000, under ultrasonic (F=28KHz) dispersion state Blow nitrogen for 30 minutes; raise the temperature to 70°C, then slowly add sodium hydroxide solution to the system, adjust the pH value of the system to about 11, and obtain a black solution containing magnetic particles, and quickly add 1% carboxymethyl after reacting for 30 minutes Chitosan-based solution, heated up to 90°C for 6 hours, terminated the reaction, left to cool to obtain a reaction liquid; separated magnetic particles under the action of an external magnetic field, and washed several times with deionized water until neutral, and then freeze-dried After treatment, carboxymethyl chitosan-modified ferric iron tetroxide magnetic nanocomposite particle powder can be obtained.

[0047] Egg yolk and egg white were separated, and then fresh egg w...

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PUM

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Abstract

The invention relates to a preparation method and applications of a magnetic nanocomposite particle, and belongs to the technical field of nano material preparation and bioactive substance separation and purification. According to the preparation method, ultrasonic-assisted chemical coprecipitation is adopted to prepare the magnetic nanocomposite particle, wherein Fe3O4 is taken as the core, and chitosan, carboxymethyl chitosan, sodium chondroitin sulfate, and citric acid are taken as shell materials. The magnetic nanocomposite particle possesses abundant surface functional groups, superparamagnetism, and excellent water dispersibility, and is suitable for application in the fields of food and medicine industry; lysozyme purity and activity preservation of a lysozyme purification method based on the magnetic nanocomposite particle are not poorer than that of conventional methods, and the lysozyme purification method based on the magnetic nanocomposite particle is simple in technology, short in production period, low in production cost, and is suitable for industrialized continuous production.

Description

technical field [0001] The invention relates to the technical field of preparation of nanomaterials and separation and purification of biologically active substances, in particular to a 3 o 4 Preparation of a magnetic nanocomposite particle with natural biopolymer polysaccharide or citric acid as the core and a shell and a method for rapidly separating and purifying lysozyme from egg white in one step. Background technique [0002] Lysozyme (Lysozyme, EC3.2.1.17) is a hydrolytic enzyme that specifically acts on the cell wall of microorganisms. It has the effect of sterilizing and breaking the wall, and is an effective antibacterial agent. Lysozyme has a wide range of applications and plays an important role in the fields of food, medicine, biology and feed industry. In recent years, in domestic and foreign markets, lysozyme is a very tight biologically active substance. [0003] So far, most of the lysozymes that have been commercialized in practical application are egg w...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01J20/22B01J20/28B01J20/30C12N9/36
Inventor 孙俊马海乐徐斌蒋蒙蒙
Owner JIANGSU UNIV
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