Adsorbent, preparation method thereof, and adsorption device for blood perfusion

Abstract

The invention provides an adsorbent, a preparation method thereof, and an adsorption device for blood perfusion. The adsorbent is prepared by bonding phosphate group in DNA molecules to diazo acetic acid esterified polystyrene-divinyl benzene microspheres through covalent bonds. The preparation method comprises the following steps: preparing polystyrene-divinyl benzene microspheres, carrying out chloromethylation, carrying out alcoholization, and performing diazo acetic acid esterification to obtain the adsorbent. The adsorption device comprises the provided adsorbent. The adsorbent has a specific absorbing performance on anti-ds-DNA antibody. Through the covalent bond, the DNA can be more firmly loaded on the solid carriers, the efficiency is higher, and the DNA will not fall off from the carrier during the process of sterilization, storage, and using. The adsorbent can be applied to SLE and can also applied to autoimmune diseases such as myasthenia gravis, Guillain-Barre syndrome, rheumatoid arthritis, hypersensitive organ transplant patients, and the like.

Description

technical field [0001] The invention relates to the fields of blood purification and polymer chemistry, in particular to an anti-double-stranded DNA antibody (anti-ds-DNA antibody), an IgG (immunoglobulin) antibody and a cytokine adsorbent that can be used for blood purification, and also relates to an adsorption Agent preparation and adsorption device using this adsorbent. Background technique [0002] Systemic lupus erythematosus (systemic lupus erythematosus, SLE) is a relatively common autoimmune disease involving multiple systems and organs. Due to the immune regulation disorder of the patient's body and the breakdown of autoimmune tolerance, a variety of autoantibodies are produced. The pathogenesis is mainly due to immune complex formation. A variety of autoantibodies can be found in SLE patients, such as anti-nuclear antibody (ANA), anti-ds(ss)-DNA antibody, anti-histone antibody, anti-RNP antibody (anti-ribonucleoprotein antibody), anti-Sm antibody, etc. Among the...

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Problems solved by technology

So far, the treatment of SLE mainly has the following problems: (1) Although the prognosis of SLE patients has been significantly improved by the treatment of glucocorticoids and immunosuppressants, many patients have adverse drug reactions and related treatment complications; ( 2) Long-term high-dose application of cytotoxic drugs and glucocorticoids can lead to consequences such as osteoporosis, hypertension, diabetes, atherosclerotic vascular disease, infection and even death, which makes the average life expectancy of SLE patients significantly shorter than that of normal people (3) Large-dose intravenous injection of human immunoglobulin is effective for drug-resistant refractory patients, but the curative effect of this method varies greatly among individuals, and there are certain adverse reactions, and the price is expensive, so the application is limited; (4 ) Plasma exchange can only delay the progress of the disease. At the same time, this method has the problems of large amount of plasma used and the risk of cross-infection, and the replaced plasma itself is also an immunostimulatory agent.

[0005] However, although there have been some schemes in the prior art that attempt to combine polystyrene ultra-macroporous resins with DNA, these schemes are all physical combinations, not only the preparation process is cumbersome and inefficient, but also the stability of the adsorbent and its resistance to The adsorption capacity of ds-DNA antibodies still needs to be improved

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