Adsorbent, preparation method thereof, and adsorption device for blood perfusion

An adsorption device and blood perfusion technology, applied in the fields of blood purification and polymer chemistry, can solve the problems of cross-infection risk, cumbersome preparation process, short average lifespan, etc., and achieve excellent biological safety and blood compatibility. The method is firm and the adsorption efficiency is improved.

Active Publication Date: 2015-12-09
JAFRON BIOMEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, the treatment of SLE mainly has the following problems: (1) Although the prognosis of SLE patients has been significantly improved by the treatment of glucocorticoids and immunosuppressants, many patients have adverse drug reactions and related treatment complications; ( 2) Long-term high-dose application of cytotoxic drugs and glucocorticoids can lead to consequences such as osteoporosis, hypertension, diabetes, atherosclerotic vascular disease, infection and even death, which makes the average life expectancy of SLE patients significantly shorter than that of normal people (3) Large-dose intravenous injection of human immunoglobulin is effective for drug-resistant refractory patients, but the curative effect of this method varies g

Method used

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  • Adsorbent, preparation method thereof, and adsorption device for blood perfusion
  • Adsorbent, preparation method thereof, and adsorption device for blood perfusion
  • Adsorbent, preparation method thereof, and adsorption device for blood perfusion

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Embodiment 1

[0043] Such as figure 2 As shown, the preparation process of polystyrene-divinylbenzene microspheres (1) is as follows: 45g styrene, 9g divinylbenzene, and 300ml toluene are mixed uniformly, then 1g of benzoyl peroxide is added, and after dissolving, 100ml Liquid paraffin and 50ml of PEG-1000 were used as porogens. Next, 1500 ml of 1.5% by mass gelatin aqueous solution was added, heated to 85° C. and then reacted for 30 hours. Then, the temperature was raised to 90° C., and all the toluene was distilled off. The balls were then boiled at 100°C for 2 hours. It is then filtered and washed several times with hot distilled water to remove the gelatin. This was followed by washing with ethanol to remove the porogen. Finally, qualified resins in the range of 0.6 mm to 0.85 mm were screened to obtain about 250 ml of polystyrene-divinylbenzene microspheres.

[0044] The chloromethylation process of polystyrene-divinylbenzene microspheres (1) is as follows: 20 g of polystyrene-di...

Embodiment 2

[0056] The amount of DNA used for ligand immobilization in this embodiment is 0.5 g, and the preparation method and evaluation experiment of the adsorbent are the same as those in the first embodiment.

Embodiment 3

[0058] The amount of DNA used for ligand immobilization in this embodiment is 0.4 g, and the preparation method and evaluation experiment of the adsorbent are the same as those in the first embodiment.

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Abstract

The invention provides an adsorbent, a preparation method thereof, and an adsorption device for blood perfusion. The adsorbent is prepared by bonding phosphate group in DNA molecules to diazo acetic acid esterified polystyrene-divinyl benzene microspheres through covalent bonds. The preparation method comprises the following steps: preparing polystyrene-divinyl benzene microspheres, carrying out chloromethylation, carrying out alcoholization, and performing diazo acetic acid esterification to obtain the adsorbent. The adsorption device comprises the provided adsorbent. The adsorbent has a specific absorbing performance on anti-ds-DNA antibody. Through the covalent bond, the DNA can be more firmly loaded on the solid carriers, the efficiency is higher, and the DNA will not fall off from the carrier during the process of sterilization, storage, and using. The adsorbent can be applied to SLE and can also applied to autoimmune diseases such as myasthenia gravis, Guillain-Barre syndrome, rheumatoid arthritis, hypersensitive organ transplant patients, and the like.

Description

technical field [0001] The invention relates to the fields of blood purification and polymer chemistry, in particular to an anti-double-stranded DNA antibody (anti-ds-DNA antibody), an IgG (immunoglobulin) antibody and a cytokine adsorbent that can be used for blood purification, and also relates to an adsorption Agent preparation and adsorption device using this adsorbent. Background technique [0002] Systemic lupus erythematosus (systemic lupus erythematosus, SLE) is a relatively common autoimmune disease involving multiple systems and organs. Due to the immune regulation disorder of the patient's body and the breakdown of autoimmune tolerance, a variety of autoantibodies are produced. The pathogenesis is mainly due to immune complex formation. A variety of autoantibodies can be found in SLE patients, such as anti-nuclear antibody (ANA), anti-ds(ss)-DNA antibody, anti-histone antibody, anti-RNP antibody (anti-ribonucleoprotein antibody), anti-Sm antibody, etc. Among the...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01J20/30C08F8/30C08F212/08A61M1/38
Inventor 董凡郭延河
Owner JAFRON BIOMEDICAL
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